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Relation between pathogenicity and genetic variation within Plasmodiophora brassicae

  • Fungal Diseases
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Abstract

The relation between diversity of pathogenicity on clubroot-resistant (CR) cultivars of Chinese cabbage (Brassica rapa subsp. pekinensis) bred in Japan and DNA polymorphisms in 17 populations of Plasmodiophora brassicae from cruciferous plants was examined by inoculation tests and random amplified polymorphic DNA (RAPD) analysis using 18 arbitrary primers. Four pathotypes (A–D) were identified after inoculation of six CR cultivars of Chinese cabbage in the 17 populations from cruciferous crops. A relatively high level of genetic diversity was also detected among these populations in the RAPD analysis. Although the four pathotypes could not be clearly differentiated using the RAPD data, most populations of three pathotypes had a consistent location on the dendrogram. All pathotype B (virulent on five cultivars except Utage 70) and D (avirulent on all cultivars) populations, which were common in incompatible interactions with cv. Utage 70, were located in a single subcluster. All five pathotype C populations (virulent only on cv. Utage 70) except for one population grouped in another single subcluster. Because four pathotype A populations (virulent on all six cultivars, races 4 and 9) fell in different subclusters, the populations may be genetically polyphyletic. Populations from cruciferous weed Cardamine flexuosa differed remarkably from those from cruciferous crops in pathogenicity on common cultivars of Chinese cabbage and turnip and C. flexuosa, but they grouped in a single cluster with all race 9 populations from crops. Race 9 populations from crops may thus be closely related to populations from the weed rather than to races 1 and 4 from crops.

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Author notes

  1. Shozo Fujiyama

    Present address: Yamaguchi Prefecture Agricultural Experiment Station, Ouchi-nagano 882, Yamaguchi, 753-0211, Japan

  2. Yoshiaki Shimizu

    Present address: Research Division, Yokohama Plant Protection Station, Ministry of Agriculture, Forestry and Fisheries Japan, Shin-yamashita 1-16-10, Naka-ku, Yokohama, 231-0801, Japan

Authors and Affiliations

  1. The United Graduate School of Agricultural Sciences, Tottori University, Koyama-machi Minami 4-101, Tottori, 680-8533, Japan

    Kohei Osaki

  2. Department of Biological and Environmental Sciences, Faculty of Agriculture, Yamaguchi University, Yoshida 1677-1, Yamaguchi, 753-8515, Japan

    Shozo Fujiyama, Akiko Nakayama, Yoshiaki Shimizu, Shin-ichi Ito & Shuhei Tanaka

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  1. Kohei Osaki
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  2. Shozo Fujiyama
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  3. Akiko Nakayama
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  4. Yoshiaki Shimizu
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  5. Shin-ichi Ito
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  6. Shuhei Tanaka
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Correspondence to Shuhei Tanaka.

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Osaki, K., Fujiyama, S., Nakayama, A. et al. Relation between pathogenicity and genetic variation within Plasmodiophora brassicae . J Gen Plant Pathol 74, 281–288 (2008). https://doi.org/10.1007/s10327-008-0102-8

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  • DOI: https://doi.org/10.1007/s10327-008-0102-8

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