Abstract
Sperm motility is known as an effective parameter in male fertility, and it depends on energy consumption. Low-level laser irradiation could increase energy supply to the cell by producing adenosine triphosphate. The purpose of this study is to evaluate how the low-level laser irradiation affects the human sperm motility. Fresh human semen specimens of asthenospermic patients were divided into four equal portions and irradiated by 830-nm GaAlAs laser irradiation with varying doses as: 0 (control), 4, 6 and 10 J/cm2. At the times of 0, 30, 45 and 60 min following irradiation, sperm motilities are assessed by means of computer-aided sperm analysis in all samples. Two additional tests [HOS and sperm chromatin dispersion (SCD) tests] were also performed on the control and high irradiated groups as well. Sperm motility of the control groups significantly decreased after 30, 45 and 60 min of irradiation, while those of irradiated groups remained constant or slightly increased by passing of time. Significant increases have been observed in doses of 4 and 6 J/cm2 at the times of 60 and 45 min, respectively. SCD test also revealed a non-significant difference. Our results showed that irradiating human sperms with low-level 830-nm diode laser can improve their progressive motility depending on both laser density and post-exposure time.
Similar content being viewed by others
References
Eddy EM, O'Brien DA (1988) The spermatozoon. In: Knobil E, Neill JD (eds) The physiology of reproduction. Raven Press, New York, pp 27–77
Rossato M, Di Virgilio F, Rizzuto R, Galeazzi G, Foresta C (2001) Intracellular calcium store depletion and acrosome reaction in human spermatozoa: role of calcium and plasma membrane potential. Mol Hum Reprod 7(2):119–128
Kujawa J, Zavodnik L, Zavodnik I, Buko V, Lapshyna A, Bryszewska M (2004) Effect of low-intensity (3.75–25 J/cm2) near-infrared (810 nm) laser radiation on red blood cell ATPase activities and membrane structure. J Clin Laser Med Surg 22(2):111–117
Frigo L, Fávero GM, Campos Lima HJ, Maria DA, Bjordal JM, Joensen J, Iversen VV, Marcos RL, Parizzoto NA, Lopes-Martins RA (2010) Low-level laser irradiation (InGaAlP-660 nm) increases fibroblast cell proliferation and reduces cell death in a dose-dependent manner. Photomed Laser Surg 28(Suppl 1):S151–156
Saracino S, Mozzati M, Martinasso G, Pol R, Canuto RA, Muzio G (2009) Superpulsed laser irradiation increases osteoblast activity via modulation of bone morphogenetic factors. Lasers Surg Med 41(4):298–304
Hemvani N, Chitnis DS, Bhagwanani NS (2005) Helium-neon and nitrogen laser irradiation accelerates the phagocytic activity of human monocytes. Photomed Laser Surg 23(6):571–574
Ocaiia Quero JM, Gomez Villamandos RJ, Moreno Millan M, Santisteban Valenzuela JM (1995) The effect of helium-neon laser irradiation on in vitro maturation and fertilization of immature bovine oocytes. Lasers Med Sci 10:113–119
Bielanski A, Hare WCD (1992) Development in vitro of bovine embryos after exposure to continuous helium-neon laser light. Theriogenology 37:192
Cohen N, Lubart R, Rubinstein S, Breitbart H (1998) Light irradiation of mouse spermatozoa: stimulation of in vitro fertilization and calcium signals. Photochem Photobiol 68(3):407–413
Ocana-Quero JM, Gomez-Villamandos R, Moreno-Millan M, Santisteban-Valenzuela JM (1997) Biological effects of helium-neon (He-Ne) laser irradiation on acrosome reaction in bull sperm cells. J Photochem Photobiol B: Biol 40:294–298
Iaffaldanoa N, Rosatoa MP, Paventib G, Pizzutob R, Gambacortaa M, Manchisia A, Passarellab S (2010) The irradiation of rabbit sperm cells with He–Ne laser prevents their in vitro liquid storage dependent damage. Anim Reprod Sci 119:123–129
Ebner T, Moser M, Yaman C, Sommergruber M, Tews G (2002) Successful birth after laser assisted immobilization of spermatozoa before intracytoplasmic injection. Fertil Steril 78(2):417–418
Corral-Baques MI, Rigau T, Rivera M, Rodriguez JE, Rigau J (2005) Effect of 655-nm diode laser on dog sperm motility. Lasers Med Sci 20:28–34
WHO (1999) Laboratory manual for the examination of human semen and sperm-cervical mucus interaction, 4th edn. Cambridge University Press, Cambridge, pp 9–10
Fernandez J, Muriel L, Rivero MT, Goyanes V, Vazquez R, Alvarez JG (2003) The sperm chromatin dispersion test: a simple method for the determination of sperm DNA fragmentation. J Androl 24:59–66
De Lamirande E, O'Flaherty C (2008) Sperm activation: role of reactive oxygen species and kinases. Biochim Biophys Acta 1784(1):106–115
Harrison KL, Sherrin DA, Gabel P, Carroll J (2008) Sperm motility enhancement with low level laser therapy. Fertil Steril 90(Suppl):S321–S322
Tadir Y, Wright WH, Vafa O, Liaw LH, Asch R, Berns MW (1991) Micromanipulation of gametes using laser microbeams. Hum Reprod 6(7):1011–1016
Zan-Bar T, Bartoov B, Segal R, Yehuda R, Lavi R, Lubart R, Avtalion RR (2005) Influence of visible light and ultraviolet irradiation on motility and fertility of mammalian and fish sperm. Photomed Laser Surg 23(6):549–555
Jeyendran RS, Van Der Ven HH, Perez-Pelaez M, Crabo BG, Zaneveld LJD (1984) Development of an assay to assess the functional integrity of the human sperm membrane and its relationship to other semen characteristics. J Reprod Fertil 70:219–228
Sato H, Landthaler M, Haina D, Chill WBS (1984) The effects of laser light on sperm motility and velocity in vitro. Andrologia 16(1):23–25
Lenzi A, Claroni F, Gandini L, Lombardo F, Barbieri C, Lino A, Dondero F (1989) Laser radiation and motility patterns of human sperm. Arch Androl 23(3):229–34
Iaffaldano N, Meluzzi A, Manchisi A, Passarella S (2005) Improvement of stored turkey semen quality as a result of He–Ne laser irradiation. Anim Reprod Sci 85:317–325
Passarella S, Casamassima E, Molinari S, Pastore D, Quagliariello E, Catalano IM, Cingolani A (1984) Increase of proton electrochemical potential and ATP synthesis in rat liver mitochondria irradiated in vitro by helium-neon laser. FEBS Lett 175(1):95–99
Oron U, Ilic S, De Taboada L, Streeter J (2007) Ga-As (808 nm) laser irradiation enhances ATP production in human neuronal cells in culture. Photomed Laser Surg 25(3):180–182
Benedicenti S, Pepe IM, Angiero F, Benedicenti A (2008) Intracellular ATP level increases in lymphocytes irradiated with infrared laser light of wavelength 904 nm. Photomed Laser Surg 26(5):451–453
Lubart R, Friedmann H, Sinyakov M, Cohen N, Breitbart H (1997) Changes in calcium transport in mammalian sperm mitochondria and plasma membranes caused by 780 nm irradiation. Lasers Surg Med 21:493–499
Krasznai Z, Krasznai T, Morisawa M, Kassai Bazsane Z, Hernadi Z, Fazekas Z, Tron L, Goda K, Marian T (2006) Role of the Na+/Ca2+ exchanger in calcium homeostasis and human sperm motility. Regul Cell Motility and the Cytoskeleton 63:66–76
Lavi R, Shainberg A, Shneyvays V, Hochauser E, Isaac A, Zinman T, Friedmann H, Lubart R (2010) Detailed analysis of reactive oxygen species induced by visible light in various cell types. Lasers Surg Med 42(6):473–480
Malik Z, Lugaci H (1987) Destruction of erythroleukaemic cells by photoactivation of endogenous porphyrins. Br J Cancer 56:589–595
Tuner J, Hode L (2002) Laser therapy: clinical practice and scientific background. Prima Books, Grangesberg, pp 68–69
Tuner J, Hode L (2002) Laser therapy: clinical practice and scientific background. Prima Books, Grangesberg, pp 95–97
Acknowledgments
We would like to thank the all staffs and patients of Royan Institute Clinical Laboratory for providing us the semen samples and analysing facilities, especially laboratory technicians Abdol-Ali Ansary and Kaveh Afraz.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Salman Yazdi, R., Bakhshi, S., Jannat Alipoor, F. et al. Effect of 830-nm diode laser irradiation on human sperm motility. Lasers Med Sci 29, 97–104 (2014). https://doi.org/10.1007/s10103-013-1276-7
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10103-013-1276-7