Abstract
A total of 180 food samples including meat (raw lean beef, frozen lean beef, and frozen chicken) and dairy products (raw milk, Zabady and Kareesh cheese) were analysed for Listeria. Isolates were differentiated using morphological, cultural, and biochemical tests and an API-Listeria kit. Zabady cheese was completely free of Listeria. The highest incidence rate (13.33%) was in frozen lean beef. Raw lean beef and milk products showed an incidence rate of 6.67%. The lowest incidence rate (3.33%) was in Kareesh cheese and frozen chicken meat samples. L. monocytogenes showed the lowest incidence rate (0.55%), isolated from one frozen lean beef sample. L. ivanovii and L. grayi showed the highest incidence rate (2.22%), isolated from 4 samples. L. innocua and L. seeligeri were positive in 3 samples (1.67%), and L. welshimeri in 2 samples (1.11%). L. monocytogenes and L. ivanovii were positive for virulence factors (hemolytic properties, and extracellular enzyme activities).
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Johnson J, Jinneman K, Stelma G, Smith BG, Lye D, Messer J, Ulaszek J, Evsen L, Gendel S, Bennett RW, Swaminathan B, Pruckler J, Steigerwalt A, Kathariou S, Yildirim S, Volokhov D, Rasooly A, Chizhikov V, Wiedmann M, Fortes E, Duvall RE, Hitchins AD. Natural atypical Listeria innocua strains with Listeria monocytogenes pathogenicity island 1 genes. Appl. Environ. Microbiol. 70: 4256–4266 (2004)
Graves LM, Helsel LO, Steigerwalt AG, Morey RE, Daneshvar MI, Roof SE, Orsi RH, Fortes ED, Millilo SR, den Bakker HC, Wiedmann M, Swaminathan B, Sauders BD. Listeria marthii sp. nov., isolated from the natural environment, Finger Lakes National Forest. Int. J. Syst. Evol. Microbiol. 60: 1280–1288 (2010)
Leclercq A, Clermont D, Bizet C, Grimont P, Le Flèche-Matéos A, Roche S, Buchrieser C, Cadet-Daniel V, Le Monnier A, Lecuit M, Allerberger F. L Listeria rocourtiae sp. nov. Int. J. Syst. Evol. Microbiol. 60: 2210–2214 (2009
Robinson RK, Batt CA, Patel PD. Encyclopedia of Food Microbiology. Academic Press, San Diego, CA, USA (2000)
Low JC, Donachie W. A review of Listeria monocytogenes and listeriosis. Vet. J. 153: 9–29 (1997).
Liu D, Lawrence M, Austin FW, Ainsworth AJ. Comparative assessment of acid, alkali and salt tolerance in Listeria monocytogenes virulent and avirulent strains. FEMS Microbiol. Lett. 243: 373–378 (2005)
Sleator RD, Gahan CGM, Hill C. A postgenomic appraisal of osmotolerance in Listeria monocytogenes. Appl. Environ. Microbiol. 69: 1–9 (2003)
Food and Drug Administration. Quantitative assessment of the relative risk to public health from food borne Listeria monocytogenes among selected categories of ready-to-eat foods. Available from: http://www.fda.gov/Food/FoodScienceResearch/RiskSafetyAssessment/ucm183966.htm. Accessed 2003.
Dhanashree B, Otta SK, Karunasagar I, Goebel W, Karunasagar I. Incidence of Listeria spp. in clinical and food samples in Mangalore, India. Food Microbiol. 20: 447–453 (2003).
WHO. Foodborne listeriosis: Report of a WHO informal working group. World Health Organization. Geneva, Switzerland. pp. 2–18 (1988)
Malik SVS, Barbuddhe SB, Chaudhari SP. Listeric infections in humans and animals in Indian Subcontinent: A review. Trop. Anim. Health. Pro. 34: 359–381 (2002)
Okutani A, Okada Y, Yamamoto S, Igimi S. Overview of Listeria monocytogenes contamination in Japan. Int. J. Food Microbiol. 93: 131–140 (2004)
Konosonoka IH, Jemeljanovs A, Osmane B, Ikauniece D, Gulbe G. Incidence of Listeria spp. in dairy cows feed and raw milk in Latvia. ISRN Vet. Sci. 2012: 1–5 (2012)
Altalhi AA. Antilisterial activity of plantaricin UG1 during manufacture of Zabady and Kareesh cheese: Two Arabian dairy products. Int. J. Biomed. Sci. 4: 319–322 (2008)
Enan G. Control of the regrowing bacteriocin resistant variants of Listeria monocytogenes LMG 10470 in vitro and in food by nisinplantaricin UG1 mixture. Biotechnology 5: 143–147 (2006).
International Organization for Standadrization 11290-1 (ISO11290-1). Microbiology of food and animal feeding stuffs-Horizontal method for the detection and enumeration of Listeria monocytogenes. ISO, Geneva, Switzerland (1996)
French Association for Standardization (AFNOR). Food microbiologydetection of Listeria monocytogenes-routine method, Vol. 08-055, AFNOR, Paris, France (1993)
Aygun O, Pehlivanlar S. Listeria spp. in the raw milk and dairy products in Antakya, Turkey. Food Control 17: 676–679 (2006)
Seeliger HPR, Jones D. The genus Listeria. Vol. II, pp. 1235–1245. In: Bergey’s Manual of Systematic Bacteriology. Sneath PHA, Mair NS, Sharpe ME, Holt JG (eds). Williams & Wilkins, Baltimore, MD, USA (1986)
Coffey A, Rombouts FM, Abee T. Influence of environmental parameters on phosphatidylcholine phospholipase C production in Listeria monocytogenes: A convenient method to differentiate L. monocytogenes from other Listeria species. Appl. Environ. Microbiol. 62: 1252–1256 (1996)
Shumi W, Hossain T, Anwar MN. Production of protease from Listeria monocytogenes. Int. J. Agri. Biol. 6: 1097–1100 (2004)
Baghel RK, Sharma R, Pandey AK. Activity of acid phosphatase in the ectomycorrhizal fungus Cantharellus tropicalis under controlled conditions. J. Trop. Forest Sci. 21: 218–222 (2009)
Bille J, Catimel B, Bannerman E, Jacquet C, Yersin MN, Caniaux I, Monget D, Rocourt J. API Listeria, a new and promising one-day system to identify Listeria isolates. Appl. Environ. Microbiol. 58: 1857–1860 (1992)
Akman D, Duran N, Digrak M. Prevalence of Listeria species in ice creams sold in the cities of Kahramanmaras and Adana. Turk. J. Med. Sci. 34: 257–262 (2004)
Harvey AR, Champe PC. Lippincott’s Illustrated Reviews: Microbiology (Lippincott’s Illustrated Reviews Series). Lippincott Williams & Wilkins, Hagerstown, MD, USA. pp. 332–353 (2007)
Barbuddhe SB, Malik SVS, Bhilegaonkar KN, Kumar P, Gupta LK. Isolation of Listeria monocytogenes and anti-listeriolysin O detection in sheep and goats. Small Ruminant Research 38: 151–155 (2000)
Gallegos JM, Vanegas MC, Albarracín Y, Máttar S, Poutou RA, Carrascal AK. Frequency of isolation of Listeria species in different retail foods in Colombia. Anim. Prod. Res. Adv. 4: 9–18 (2008)
Fenlon DR, Wilson J, Donachie W. The incidence and level of Listeria monocytogenes contamination of food sources at primary production and initial processing. J. Appl. Bacteriol. 81: 641–650 (1996)
Edson DC, Empson S, Massey LD. Pathogen detection in food microbiology laboratories: An analysis of qualitative proficiency test data, 199–2007. J. Food Safety 29: 521–530 (2009)
Poltronieri P, de Blasi MD, D’Urso OF. Detection of Listeria monocytogenes through real-time PCR and biosensor methods. Plant Soil Environ. 55: 363–369 (2009)
Atil E, Ertas HB, Ozbey G. Isolation and molecular characterization of Listeria spp. from animals, food and environmental samples. Vet. Med. 56: 386–394 (2011)
Beak SY, Lim YS, Lee DH, Min KH, Kim CM. Incidence and characterization of Listeria monocytogenes from domestic and imported foods in Korea. J. Food Protect. 63: 186–189 (2000)
Hudson JA, Mott SJ, Delacy KM, Edridge AL. Incidence and coincidence of Listeria spp. motile aeromonads and Yersinia enterocolitica on ready-to-eat flesh foods. Int. J. Food Microbiol. 16: 99–108 (1992)
Rocourt J, Hogue A, Toyofuku H, Jacquet C, Schlundt J. Listeria and listeriosis: Risk assessment as a new tool to unravel a multifaceted problem. Am. J. Infect. Control 29: 225–227 (2001)
Gaya P, Saralegui C, Medina M. Occurrence of Listeria monocytogenes and other Listeria spp. in raw caprine milk. J. Dairy Sci. 79: 1936–1941 (1996)
Yücel N, Cýtak S, Önder MO. Prevalence and antibiotic resistance of Listeria species in meat products in Ankara, Turkey. Food Microbiol. 22: 241–245 (2005)
Nufer U, Stephan R, Tasara T. Growth characteristics of Listeria monocytogenes,L. welshimeri and L. innocua strains in both cultures and slice bologna-type product at 4 and 7 degrees C. Food Microbiol. 24: 444–451 (2007)
Groves RD, Welshimer HJ. Separation of pathogenic from apathogenic Listeria monocytogenes by three in vitro reactions. J. Clin. Microbiol. 5: 559–563 (1977)
Hof H. Virulence of different strains of Listeria monocytogenes serovar 1/2a. Med. Microbiol. Immun. 173: 207–218 (1984)
Geoffroy C, Gaillard J-L, Alouf JE, Berche P. Purification, characterization, and toxicity of the sulfhydryl-activated hemolysin listeriolysin O from Listeria monocytogenes. Infect. Immun. 55: 1641–1646 (1987)
Vázquez-Boland J-A, Dominguez L, Rodriguez-Ferri E-F, Suarez G. Purification and characterization of two Listeria ivanovii cytolysins, a sphingomyelinas C and a thiol-activated toxin (ivanolysin O). Infect. Immun. 57: 3928–3935 (1989)
Sheehan B, Kocks C, Dramsi S, Gouin E, Klarsfield, AD, Mengaud J, Cossart P. Molecular and genetic determinants of the Listeria monocytogenes infectious process. Curr. Top. Microbiol. Immun. 192: 187–216 (1994)
Vázquez-Boland J-A, Kocks C, Dramsi S, Geoffroy C, Mengaud J, Cossart P. Nucleotide sequence of the lecithinase operon of Listeria monocytogenes and possible role in cell-to-cell spread. Infec. Immun. 60: 219–230 (1992)
Poyart C, Abachin E, Razafimanantsoa I, Berche P. The zinc metalloprotease of Listeria monocytogenes is required for maturation of phosphatidylcholine phospholipase C: Direct evidence obtained by gene complementation. Infect. Immun. 61: 1576–1580 (1993)
Titball RW. Bacterial phospholipases C. Microbiol. Rev. 57: 347–366 (1993)
Raveneau J, Geoffroy C, Beretti JL, Gaillard JL, Alouf JE, Berche P. Reduced virulence of a Listeria monocytogenes phosphpholipase-deficient mutant obtained by transposon insertion into the zinc metalloprotease gene. Infect. Immun. 60: 916–921 (1992)
Barclay R, Threlfall DR, Leighton I. Separation and properties of the haemolysins and extracellular enzymes of Listeria monocytogenes. J. Med. Microbiol. 30: 119–127 (1989)
Barclay R, Threlfall DR, Leighton I. Haemolysins and extracellular enzymes of Listeria monocytogenes and L. ivanovii. J. Med. Microbiol. 30: 111–118 (1989)
Sword CP, Kingdon GC. Listeria monocytogenes toxin. pp. 357–377. In: Microbial toxins-bacterial protein toxins. Kadis S, Montie C, Ajl SJ (eds). Academic Press, London, UK (1971)
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Ismaiel, A.AR., Ali, A.ES. & Enan, G. Incidence of Listeria in Egyptian meat and dairy samples. Food Sci Biotechnol 23, 179–185 (2014). https://doi.org/10.1007/s10068-014-0024-5
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DOI: https://doi.org/10.1007/s10068-014-0024-5