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Cyanidin-3-O-(2″-xylosyl)-glucoside, an anthocyanin from Siberian ginseng (Acanthopanax senticosus) fruits, inhibits UVB-induced COX-2 expression and AP-1 transactivation

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Abstract

Cyanidin-3-O-(2″-xylosyl)-glucoside (C-3-O-(2″-xylosyl)-G) was analyzed as an active constituent from the fruit of Siberian ginseng (Acanthopanax senticosus) using a HPLC diode array detection-electrospray ionization/mass spectrometry analysis system and the effect of C-3-O-(2″-xylosyl)-G on UVB-induced inflammatory signaling in JB6 P+ cells was investigated. C-3-O-(2″-xylosyl)-G inhibited UVB-induced cyclooxygenase (COX)-2 expression and promoter binding activity in JB6 P+ cells and JB6 P+ cells stably transfected with the COX-2 luciferase reporter plasmid. It inhibited both the UVB-induced activator protein-1 (AP-1) and nuclear factor (NF)-κB transactivation in JB6 P+ cells stably transfected with the AP-1 and NF-κB luciferase reporter plasmids. Additionally, C-3-O-(2″-xylosyl)-G significantly suppressed UVB-induced upregulated phosphorylation of c-Jun N terminal kinase, MEK/extracellular signaling kinase, and mitogen activated protein kinase kinase (MAPKK) 3/6 in JB6 P+ cells. These results indicate that C-3-O-(2″-xylosyl)-G may be a promising chemopreventive material that acts by suppressing COX-2 expression and AP-1 and NF-κB transactivation and JNK, MAPKK3/6, and MEK/ERK1/2 phosphorylation.

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Authors and Affiliations

  1. WCU Biomodulation Major, Department of Agricultural Biotechnology and Center for Food and Bioconvergence, Seoul National University, Seoul, 151-921, Korea

    Sung Keun Jung, Tae-Gyu Lim, Sang Gwon Seo, Hyong Joo Lee & Ki Won Lee

  2. Functional Food Resources Research Group, Korea Food Research Institute, Seongnam, Gyeonggi, 463-746, Korea

    Sung Keun Jung

  3. Advanced Institute of Convergence Technolgy, Seoul National University, Suwon, Gyeonggi, 443-270, Korea

    Tae-Gyu Lim, Hyong Joo Lee & Ki Won Lee

  4. Department of Herbal Medicine Resource, Kangwon National University, Samcheok, Gangwon, 245-907, Korea

    Young-Sun Hwang & Myoung-Gun Choung

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  1. Sung Keun Jung
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  2. Tae-Gyu Lim
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  3. Sang Gwon Seo
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  7. Ki Won Lee
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Correspondence to Ki Won Lee.

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Jung, S.K., Lim, TG., Seo, S.G. et al. Cyanidin-3-O-(2″-xylosyl)-glucoside, an anthocyanin from Siberian ginseng (Acanthopanax senticosus) fruits, inhibits UVB-induced COX-2 expression and AP-1 transactivation. Food Sci Biotechnol 22, 507–513 (2013). https://doi.org/10.1007/s10068-013-0108-7

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  • DOI: https://doi.org/10.1007/s10068-013-0108-7

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