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Genetic determination and localization of multiple bacteriocins produced by Enterococcus faecium CWBI-B1430 and Enterococcus mundtii CWBI-B1431

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Abstract

Enterococcus faecium CWBI-B1430 and Enterococcus mundtii CWBI-B1431 from artisanal-produced Peruvian cheeses showed the presence of 4 putative bacteriocin genes: enterocin A, enterocin B, enterocin P, and mundticin KS. The multiple bacteriocin producer E. faecium CWBI-B1430 presented 1 plasmid of 34.6 kb, whereas E. mundtii CWBI-B1431 contained 1 plasmid of 11.0 kb. The structural gene responsible for mundticin KS production was located on 5.6 and 3.1 kb HindIII plasmid fragments. The reverse transcription-PCR analysis showed the expression of the bacteriocin genes enterocin A, enterocin B, and mundticin KS in E. faecium CWBI-B1430 and the bacteriocin genes enterocin P and mundticin KS in E. mundtii CWBI-B1431. To our knowledge, this is the first report of the expression of mundticin KS in E. faecium and enterocin P in E. mundtii.

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Authors and Affiliations

  1. Instituto de Biotecnología, Universidad Nacional Agraria La Molina, Av. La Molina s/n., Lima, Perú

    Ana Aguilar-Galvez & David Campos

  2. Gembloux Agro-Bio Tech. Unit of Bio-Industry, University of Liège, Passage des Déportés 2, B-5030, Gembloux, Belgium

    Ana Aguilar-Galvez, Robin Dubois-Dauphin & Philippe Thonart

Authors
  1. Ana Aguilar-Galvez
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  2. Robin Dubois-Dauphin
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  3. David Campos
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  4. Philippe Thonart
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Correspondence to David Campos.

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Aguilar-Galvez, A., Dubois-Dauphin, R., Campos, D. et al. Genetic determination and localization of multiple bacteriocins produced by Enterococcus faecium CWBI-B1430 and Enterococcus mundtii CWBI-B1431. Food Sci Biotechnol 20, 289–296 (2011). https://doi.org/10.1007/s10068-011-0041-6

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