Molecules and Cells

, Volume 33, Issue 2, pp 111–116

Identification and characterization of a noncoding RNA at the mouse Pcna locus

  • Ji-Yeon Lee
  • Abdul Aziz Khan
  • Hyehyun Min
  • Xinnan Wang
  • Myoung Hee Kim
Article

DOI: 10.1007/s10059-012-2164-x

Cite this article as:
Lee, JY., Khan, A.A., Min, H. et al. Mol Cells (2012) 33: 111. doi:10.1007/s10059-012-2164-x

Abstract

AK007836 encodes a noncoding RNA (ncRNA) consisting of 2 exons. Since AK007836 is located just upstream of Pcna and transcribed in the opposite direction to that of Pcna, we analyzed its expression pattern. Both ncRNA and Pcna expressions were detected in in vitro and in vivo cells, showing a positive correlation. A 177 bp region separating the first exons of Pcna and AK007836 has a bidirectional promoter activity. When the expression of ncRNA was reduced by siRNA, Pcna expression was also reduced in normal cells, but not in cancer cells. These results suggest that the ncRNA is divergently transcribed from the bidirectional promoter, positively regulating the neighboring protein-coding Pcna gene transcription, and this regulatory function is somehow disrupted in cancer cells.

Keywords

bidirectional promoter co-expression gene regulation non-coding RNA Pcna 

Copyright information

© The Korean Society for Molecular and Cellular Biology and Springer Netherlands 2012

Authors and Affiliations

  • Ji-Yeon Lee
    • 1
  • Abdul Aziz Khan
    • 1
  • Hyehyun Min
    • 1
    • 2
  • Xinnan Wang
    • 1
  • Myoung Hee Kim
    • 1
    • 2
  1. 1.Department of Anatomy, Embryology LaboratoryYonsei University College of MedicineSeoulKorea
  2. 2.Brain Korea 21 Project for Medical ScienceYonsei University College of MedicineSeoulKorea
  3. 3.Department of Life SciencePohang University of Science and TechnologyPohangKorea

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