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Genome organization and host range of a Brazilian isolate of johnsongrass mosaic virus

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Abstract

This work reports the complete genome sequence, production of a polyclonal antiserum, and host range of a Brazilian strain of johnsongrass mosaic virus (JGMV) found infecting Panicum maximum in the state of São Paulo, Brazil. The complete genome sequence of this potyvirus, comprising 9874 nucleotides, showed 82 % amino acid sequence identity in the polyprotein to that of an isolate of JGMV from Australia. The experimental host range of this virus included mainly fodder species. Cultivated species such as rice, oats, sugarcane, rye, corn and wheat were not infected, suggesting that current isolates of this potyvirus do not represent a threat to these crops in Brazil.

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Acknowledgments

We acknowledge Prof. Luiz L. Coutinho for the access to computer resources for genome analysis.

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Authors and Affiliations

  1. Departamento de Fitopatologia e Nematologia, Escola Superior de Agricultura Luiz de Queiroz, Universidade de São Paulo, Piracicaba, SP, Brazil

    Viviana Marcela Camelo-García, Elliot Watanabe Kitajima & Jorge A. M. Rezende

  2. Departamento de Genética e Biologia Evolutiva, Instituto de Biociências, USP, São Paulo, SP, Brazil

    Sónia Cristina da Silva Andrade

  3. Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, GPO Box 267, Brisbane, QLD, 4001, Australia

    Andrew D. W. Geering

Authors
  1. Viviana Marcela Camelo-García
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  2. Sónia Cristina da Silva Andrade
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  3. Andrew D. W. Geering
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  4. Elliot Watanabe Kitajima
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  5. Jorge A. M. Rezende
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Corresponding author

Correspondence to Jorge A. M. Rezende.

Electronic supplementary material

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705_2016_2772_MOESM1_ESM.docx

Supplementary Figure 1 Genome organization of johnsongrass mosaic virus isolated from P. maximum cv. Mombaça. The two lines at the extremities represent the 5′ and 3′ untranslated regions. The larger box represents the unique open reading frame. The proteins resulting from post-translational processing of the polyprotein are indicated in the smaller boxes. The numbers above indicate the beginning of each gene. The likely cleavage sites are indicated at the bottom. (DOCX 60 kb)

705_2016_2772_MOESM2_ESM.docx

Supplementary Figure 2 Detection of johnsongrass mosaic virus by RT-PCR. The expected PCR product was 1,121 bp. M, molecular weight marker 1 kb Plus DNA Ladder (Invitrogen). JGMV-inoculated species: (1) Sorghum bicolor x S. sudanense BRS 802, (2) S. bicolor BRS 509, (3) Echinochloa settler, (4) S. verticilliflorum, (5) P. maximum cv. Mombaça, (6) johnsongrass, (7) healthy P. maximum cv. Mombaça, and (8) sugarcane infected with SCMV. (DOCX 69 kb)

705_2016_2772_MOESM3_ESM.docx

Supplementary Table 1 PTA-ELISA absorbance readings for different species/varieties of Poaceae mechanically inoculated with a Brazilian isolate of johnsongrass mosaic virus. (DOCX 16 kb)

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Camelo-García, V.M., da Silva Andrade, S.C., Geering, A.D.W. et al. Genome organization and host range of a Brazilian isolate of johnsongrass mosaic virus. Arch Virol 161, 1335–1341 (2016). https://doi.org/10.1007/s00705-016-2772-4

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  • DOI: https://doi.org/10.1007/s00705-016-2772-4

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