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A member of the virus family Narnaviridae from the plant pathogenic oomycete Phytophthora infestans

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Abstract

A virus that has properties consistent with inclusion in the virus family Narnaviridae was described in Phytophthora infestans, the oomycete that caused the Irish potato famine. The genome of phytophthora infestans RNA virus 4 (PiRV-4) is 2,984 nt with short complementary terminal sequences and a single open reading frame predicted to encode an RNA-dependent RNA polymerase (RdRp) most closely related to saccharomyces cerevisiae narnavirus 20S (ScNV-20S) and ScNV-23S, the members of the genus Narnavirus, family Narnaviridae. This report constitutes the first description of a member of the family Narnaviridae from a host taxon outside of the kingdom Fungi.

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References

  1. Altschul SF, Madden TL, Schaffer AA, Zhang JH, Zhang Z, Miller W, Lipman DJ (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 25:3389–3402

    Article  PubMed  CAS  Google Scholar 

  2. Attoui H, Billoir F, Cantaloube JF, Biagini P, de Micco P, de Lamballerie X (2000) Strategies for the sequence determination of viral dsRNA genomes. J Virol Methods 89:147–158

    Article  PubMed  CAS  Google Scholar 

  3. Buck KW, Esteban R, Hillman BI (2005) Family Narnaviridae. In: Fauquet CM, Mayo MA, Maniloff J, Desselberger U, Ball LA (eds) Virus taxonomy: eighth report of the international committee for the taxonomy of viruses. Academic Press, New York, pp 715–757

    Google Scholar 

  4. Cai G, Fry WE, Hillman BI, Myers K (2009) An RNA virus from Phytophthora infestans with no apparent similarity to known viruses. Phytopathology 99:S18

    Google Scholar 

  5. Cai G, Myers K, Hillman BI, Fry WE (2009) A novel virus of the late blight pathogen, Phytophthora infestans, with two RNA segments and a supergroup 1 RNA-dependent RNA polymerase. Virology 392:52–61

    Article  PubMed  CAS  Google Scholar 

  6. Edgar RC (2004) MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res 32:1792–1797

    Article  PubMed  CAS  Google Scholar 

  7. Esteban LM, Rodriguez-Cousino N, Esteban R (1992) T double-stranded RNA (dsRNA) sequence reveals that T and W dsRNAs form a new RNA family in Saccharomyces cerevisiae. Identification of 23 S RNA as the single-stranded form of T dsRNA. J Biol Chem 267:10874–10881

    PubMed  CAS  Google Scholar 

  8. Ghabrial SA, Suzuki N (2009) Viruses of plant pathogenic fungi. Annu Rev Phytopathol 47:353–384

    Article  PubMed  CAS  Google Scholar 

  9. Goodwin SB, Drenth A, Fry WE (1992) Cloning and genetic analyses of two highly polymorphic, moderately repetitive nuclear DNAs from Phytophthora infestans. Curr Genet 22:107–115

    Article  PubMed  CAS  Google Scholar 

  10. Goodwin SB (1997) The population genetics of Phytophthora. Phytopathology 87:462–473

    Article  PubMed  CAS  Google Scholar 

  11. Hong Y, Cole TE, Brasier CM, Buck KW (1998) Evolutionary relationships among putative RNA-dependent RNA polymerases encoded by a mitochondrial virus-like RNA in the dutch elm disease fungus, Ophiostoma novo-ulmi, by other viruses and virus-like RNAs and by the Arabidopsis mitochondrial genome. Virology 246:158–169

    Article  PubMed  CAS  Google Scholar 

  12. Hong Y, Dover SL, Cole TE, Brasier CM, Buck KW (1999) Multiple mitochondrial viruses in an isolate of the dutch elm disease fungus Ophiostoma novo-ulmi. Virology 258:118–127

    Article  PubMed  CAS  Google Scholar 

  13. Karlovsky P, Fartmann B (1992) Genetic-code and phylogenetic origin of oomycetous mitochondria. J Mol Evol 34:254–258

    Article  PubMed  CAS  Google Scholar 

  14. Koonin EV, Dolja VV (1993) Evolution and taxonomy of positive-strand RNA viruses: Implications of comparative analysis of amino acid sequences. Crit Rev Biochem Mol Biol 28:375–430

    Article  PubMed  CAS  Google Scholar 

  15. Lakshman DK, Jian J, Tavantzis SM (1998) A double-stranded RNA element from a hypovirulent strain of Rhizoctonia solani occurs in DNA form and is genetically related to the pentafunctional AROM protein of the shikimate pathway. Proc Natl Acad Sci 95:6425–6429

    Article  PubMed  CAS  Google Scholar 

  16. Lambden PR, Cooke SJ, Caul EO, Clarke IN (1992) Cloning of noncultivatable human rotavirus by single primer amplification. J Virol 66:1817–1822

    PubMed  CAS  Google Scholar 

  17. Matsumoto Y, Wickner RB (1991) Yeast 20 S RNA replicon. Replication intermediates and encoded putative RNA polymerase. J Biol Chem 266:12779–12783

    PubMed  CAS  Google Scholar 

  18. Milgroom MG, Hillman BI (2011) The ecology and evolution of fungal viruses. In: Hurst CJ (ed) Studies in Viral Ecology. John Wiley & Sons, Inc, New York, pp 221–257

    Google Scholar 

  19. Morris TJ, Dodds JA (1979) Isolation and analysis of double-stranded RNA from virus-infected plant and fungal tissue. Phytopathology 69:854–858

    Article  CAS  Google Scholar 

  20. Park Y, Chen X, Punja ZK (2006) Molecular and biological characterization of a mitovirus in Chalara elegans (Thielaviopsis basicola). Phytopathology 96:468–479

    Article  PubMed  CAS  Google Scholar 

  21. Polashock JJ, Hillman BI (1994) A small mitochondrial double-stranded (ds) RNA element associated with a hypovirulent strain of the chestnut blight fungus and ancestrally related to yeast cytoplasmic T and W dsRNAs. Proc Natl Acad Sci USA 91:8680–8684

    Article  PubMed  CAS  Google Scholar 

  22. Rastgou M, Habibi MK, Izadpanah K, Masenga V, Milne RG, Wolf YI, Koonin EV, Turina M (2009) Molecular characterization of the plant virus genus Ourmiavirus and evidence of inter-kingdom reassortment of viral genome segments as its possible route of origin. J Gen Virol 90:2525–2535

    Article  PubMed  CAS  Google Scholar 

  23. Rodriguez-Cousino N, Solorzano A, Fujimura T, Esteban R (1998) Yeast positive-stranded virus-like RNA replicons - 20 S and 23 S RNA terminal nucleotide sequences and 3′ end secondary structures resemble those of RNA coliphages. J Biol Chem 273:20363–20371

    Article  PubMed  CAS  Google Scholar 

  24. Sachay DJ, Hudspeth DSS, Nadler SA, Hudspeth MES (1993) Oomycete mtDNA: Phytophthora genes for cytochrome c oxidase use an unmodified genetic code and encode proteins most similar to those of plants. Exp Mycol 17:7–23

    Article  CAS  Google Scholar 

  25. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular Cloning. In: A Laboratory Mannual, 2nd edn. Cold Spring Harbor Laboratory Press

  26. Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol. doi:10.1093/molbev/msr1121

    Google Scholar 

  27. Wickner RB (2001) Viruses of yeasts, fungi, and parasitic microorganisms. In: Knipe DM, Howley PM (eds) Fields Virology. Lippencott Williams & Wilkens, Philadelphia, pp 629–658

    Google Scholar 

  28. Wu M, Zhang L, Li G, Jiang D, Ghabrial SA (2010) Genome characterization of a debilitation-associated mitovirus infecting the phytopathogenic fungus Botrytis cinerea. Virology 406:117–126

    Article  PubMed  CAS  Google Scholar 

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Acknowledgments

The authors wish to thank the New Jersey Agricultural Experiment Station and College of Agriculture and Life Sciences at Cornell University for partial funding for this project.

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Authors and Affiliations

  1. Department of Plant Biology and Pathology, Rutgers, The State University of New Jersey, New Brunswick, NJ, 08901, USA

    Guohong Cai & Bradley I. Hillman

  2. Department of Plant Pathology and Plant-Microbe Biology, Cornell University, Ithaca, NY, 14853, USA

    Kevin Myers & William E. Fry

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  1. Guohong Cai
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  2. Kevin Myers
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  3. William E. Fry
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  4. Bradley I. Hillman
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Correspondence to Guohong Cai or Bradley I. Hillman.

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Cai, G., Myers, K., Fry, W.E. et al. A member of the virus family Narnaviridae from the plant pathogenic oomycete Phytophthora infestans . Arch Virol 157, 165–169 (2012). https://doi.org/10.1007/s00705-011-1126-5

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  • DOI: https://doi.org/10.1007/s00705-011-1126-5

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