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Synergistic interactions between Glomus mosseae and Bradyrhizobium japonicum in enhancing proton release from nodules and hyphae

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Abstract

Soybean (Glycine max L. Merr.) seedlings were inoculated with Glomus mosseae (GM) and Bradyrhizobium japonicum (BJ) together or separately to study the effect of interactions on net H+ effluxes of nodules or extraradical hyphae by in vivo vibrating electrode techniques. GM promoted three-fold the H+ effluxes of nodules on mycorrhizal lateral roots and BJ increased eight-fold the net H+ effluxes of hyphae developing in the vicinity of nodules on lateral roots. Increments in plant P content were positively and linearly correlated with the net H+ efflux of nodules and hyphae. It is concluded that increased H+ effluxes of nodules resulted from enhanced nitrogenase activities induced by the presence of the AM fungus in lateral roots. The results point to additive effects of interactions between mycorrhizal fungi and rhizobia in increasing the extent of acidification of the “nodulesphere” and the hyposphere.

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Acknowledgments

The study was partly supported by the National Natural Science Foundation of China (the major program of 30890132 and the Science Fund for Creative Research Groups of 30821003), Chinese University Scientific Fund (2009TD15), and National Basic Research Program of China (2007CB109308). We acknowledge the assistance of Professor Alastair Fitter and Zed Rangel during early writing stages. We would also like to thank Professor Vivienne Gianinazzi-Pearson and two anonymous reviewers for their constructive comments and revisions.

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Authors and Affiliations

  1. Key Laboratory of Plant–Soil Interactions, Ministry of Education, College of Resource and Environmental Science, China Agricultural University, Beijing, 100193, China

    Xiaodong Ding, Fang Wang, Junhua Gao, Fusuo Zhang & Gu Feng

  2. State Key Laboratories for Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, 100193, China

    Xinhua Sui

  3. Northern Research Station, USDA Forest Service and School of Forest Resources and Environmental Science, Michigan Technological University, Houghton, MI, 49931, USA

    Xinhua He

  4. Institute of Agricultural Resources & Regional Planning, CAAS, Beijing, 100081, China

    Juncheng Yang

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  1. Xiaodong Ding
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  2. Xinhua Sui
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  3. Fang Wang
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  4. Junhua Gao
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  5. Xinhua He
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  8. Gu Feng
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Correspondence to Gu Feng.

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Information S1

Measurement of net H+ efflux using the non-invasive micro-test technique (DOC 16.0 kb)

Information S2

Effects of G. mosseae (GM) and/or B. japonicum (BJ) inoculation on P and N concentration (mg g−1 DW) in shoots, roots, and root nodules of soybeans at 56 days after sowing (DOC 30 kb)

Information S3

Net H+ effluxes (pmol cm−2 s−1) in taproot (a) and lateral roots (b) under different treatments. Values are means ± SE (n = 4). Different letters above the bars indicate significant differences at P = 0.05. Abbreviations: LR lateral roots; LR+ lateral roots with nodules; LR− lateral roots without nodules; GM sole inoculated with G. mosseae; BJ sole inoculation with B. japonicum; GM/BJ dual inoculated with G. mosseae + B. japonicum (DOC 33 kb)

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Ding, X., Sui, X., Wang, F. et al. Synergistic interactions between Glomus mosseae and Bradyrhizobium japonicum in enhancing proton release from nodules and hyphae. Mycorrhiza 22, 51–58 (2012). https://doi.org/10.1007/s00572-011-0381-3

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  • DOI: https://doi.org/10.1007/s00572-011-0381-3

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