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Oxidant and enzymatic antioxidant status (gene expression and activity) in the brain of chickens with cold-induced pulmonary hypertension

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Abstract

To evaluate oxidant and antioxidant status of the brain (hindbrain, midbrain, and forebrain) in chickens with cold-induced pulmonary hypertension, the measurements of lipid peroxidation, protein oxidation, antioxidant capacity, enzymatic activity, and gene expression (for catalase, glutathione peroxidase, and superoxide dismutases) were done. There were high lipid peroxidation/protein oxidation and low antioxidant capacity in the hindbrain of cold-induced pulmonary hypertensive chickens compared to control (P < 0.05). In the hypertensive chickens, superoxide dismutase activity was decreased (forebrain, midbrain, and hindbrain), while catalase activity was increased (forebrain and midbrain) (P < 0.05). Glutathione peroxidase activity did not change. Relative gene expression of catalase and superoxide dismutases (1 and 2) was downregulated, while glutathione peroxidase was upregulated in the brain of the cold-induced pulmonary hypertensive chickens. Probably, these situations in the oxidant and antioxidant status of the brain especially hindbrain may change its function at cardiovascular center and sympathetic nervous system to exacerbate pulmonary hypertension.

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Acknowledgments

The research presented in this paper was supported by grants provided by the Applied Research Center and the Vice Chancellor for Research of Shahrekord University.

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Authors and Affiliations

  1. Department of Basic Sciences, Physiology Division, Faculty of Veterinary Medicine, Shahrekord University, P.O. BOX 115, Saman Road, Shahrekord, Iran

    Hossein Hassanpour

  2. Department of Biology, Faculty of Science, Shahrekord University, P.O. BOX 115, Saman Road, Shahrekord, Iran

    Valiallah Khalaji-Pirbalouty & Leila Nasiri

  3. Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahrekord University, P.O. BOX 115, Saman Road, Shahrekord, Iran

    Abdonnaser Mohebbi & Shahab Bahadoran

Authors
  1. Hossein Hassanpour
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  2. Valiallah Khalaji-Pirbalouty
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  3. Leila Nasiri
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  4. Abdonnaser Mohebbi
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  5. Shahab Bahadoran
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Correspondence to Hossein Hassanpour.

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Hassanpour, H., Khalaji-Pirbalouty, V., Nasiri, L. et al. Oxidant and enzymatic antioxidant status (gene expression and activity) in the brain of chickens with cold-induced pulmonary hypertension. Int J Biometeorol 59, 1615–1621 (2015). https://doi.org/10.1007/s00484-015-0968-z

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  • DOI: https://doi.org/10.1007/s00484-015-0968-z

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