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Biobutanol production from rice bran and de-oiled rice bran by Clostridium saccharoperbutylacetonicum N1-4

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Abstract

Rice bran (RB) and de-oiled rice bran (DRB) have been treated and used as the carbon source in acetone–butanol–ethanol (ABE) production using Clostridium saccharoperbutylacetonicum N1-4. The results showed that pretreated DRB produced more ABE than pretreated RB. Dilute sulfuric acid was the most suitable treatment method among the various pretreatment methods that were applied. The highest ABE obtained was 12.13 g/L, including 7.72 g/L of biobutanol, from sulfuric acid. The enzymatic hydrolysate of DRB (ESADRB), when treated with XAD-4 resin, resulted in an ABE productivity and yield of 0.1 g/L h and 0.44 g/g, respectively. The results also showed that the choice of pretreatment method for RB and DRB is an important factor in butanol production.

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Acknowledgments

We would like to thank Prof. Dr Yoshino Sadazo, Kyushu University, Japan, who provided us with C. saccharoperbutylacetonicum N1-4. This research was supported by the UKM-GUP-KPB -08-32-128 grant.

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Authors and Affiliations

  1. School of Bioscience and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM, Bangi, Selangor, Malaysia

    Najeeb Kaid Nasser Al-Shorgani & Wan Mohtar Wan Yusoff

  2. Department of Chemical and Process Engineering, Faculty of Engineering, Universiti Kebangsaan Malaysia, 43600 UKM, Bangi, Selangor, Malaysia

    Mohd Sahaid Kalil

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  1. Najeeb Kaid Nasser Al-Shorgani
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  2. Mohd Sahaid Kalil
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  3. Wan Mohtar Wan Yusoff
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Correspondence to Najeeb Kaid Nasser Al-Shorgani.

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Al-Shorgani, N.K.N., Kalil, M.S. & Yusoff, W.M.W. Biobutanol production from rice bran and de-oiled rice bran by Clostridium saccharoperbutylacetonicum N1-4. Bioprocess Biosyst Eng 35, 817–826 (2012). https://doi.org/10.1007/s00449-011-0664-2

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