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Distribution of pectic epitopes in cell walls of the sugar beet root

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Abstract

Immunolabelling techniques with antibodies specific to partially methyl-esterified homogalacturonan (JIM5: unesterified residues flanked by methylesterified residues. JIM7: methyl-esterified residues flanked by unesterified residues), a blockwise de-esterified homogalacturonan (2F4), 1,4-galactan (LM5) and 1,5-arabinan (LM6) were used to map the distribution of pectin motifs in cell walls of sugar beet root (Beta vulgaris). PME and alkali treatments of sections were used in conjunction with JIM5-7 and 2F4. The JIM7 epitope was abundant and equally distributed in all cells. In storage parenchyma, the JIM5 epitope was restricted to some cell junctions and the lining of intercellular spaces while in vascular tissues it occurred at cell junctions in some phloem walls and in xylem derivatives. After secondary wall formation, the JIM5 epitope was restricted to inner cell wall regions between secondary thickenings. The 2F4 epitope was not detected without de-esterification treatment. PME treatments prior to the use of 2F4 indicated that HG at cell corners was not acetylated. The LM5 epitope was mainly present in the cambial zone and when present in storage parenchyma, it was restricted to the wall region closest to the plasma membrane. The LM6 epitope was widely distributed throughout primary walls but was more abundant in bundles than in medullar ray tissue and storage parenchyma. These data show that the occurrence of oligosaccharide motifs of pectic polysaccharides are spatially regulated in sugar beet root cell walls and that the spatial patterns vary between cell types suggesting that structural variants of pectic polymers are involved in the modulation of cell wall properties.

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Abbreviations

GalA:

Galacturonic acid

Ara:

Arabinose

Gal:

Galactose

Rha:

Rhamnose

HG:

Homogalacturonan

RGI:

Rhamnogalacturonan I

PME:

Pectin methylesterase

PAE:

Pectin acetylesterase

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Acknowledgements

The authors would like to thank Dr S Lévigne and Dr M-C Ralet, both from Unité de Recherche sur les Polysaccharides, leurs Organisations et Interactions, Institut National de la Recherche Agronomique, Nantes, France, for they kind gifts of series of sugar beet pectins. The authors thank Prof. P. Van Cutsem from Unité de Recherche en Biologie Cellulaire, Facultés Universitaires Notre Dame de la Paix, Namur, Belgium, for the generous gift of 2F4 monoclonal antibody. They are grateful to C. Nicolas, Institut National de la Recherche Agronomique, Nantes, France for photographic assistance. F. Guillemin gratefully acknowledges the Institut National de la Recherche Agronomique and the Région Pays de Loire for their support through a doctoral fellowship.

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Authors and Affiliations

  1. Institut National de la Recherche Agronomique, Unité de Recherche sur les Polysaccharides, leurs Organisations et Interactions, rue de la Géraudière, BP 71627, 44316, Nantes cedex 03, France

    Florence Guillemin, Fabienne Guillon, Estelle Bonnin, Marie-Françoise Devaux, Thérèse Chevalier & Jean-François Thibault

  2. Centre for Plant Sciences, University of Leeds, Leeds, LS2 9JT, UK

    J Paul Knox

  3. Unité de Recherche en Biologie Cellulaire et Moléculaire Végétale, Facultés Universitaires Notre-Dame de la Paix of Namur, rue de Bruxelles 61, 5000, Namur, Belgium

    Françoise Liners

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  1. Florence Guillemin
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  2. Fabienne Guillon
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  3. Estelle Bonnin
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  4. Marie-Françoise Devaux
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  5. Thérèse Chevalier
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  6. J Paul Knox
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  7. Françoise Liners
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  8. Jean-François Thibault
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Correspondence to Fabienne Guillon.

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Guillemin, F., Guillon, F., Bonnin, E. et al. Distribution of pectic epitopes in cell walls of the sugar beet root. Planta 222, 355–371 (2005). https://doi.org/10.1007/s00425-005-1535-3

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