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Pomegranate and green tea extracts protect against ER stress induced by a high-fat diet in skeletal muscle of mice

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Abstract

Purpose

We tested the hypothesis that polyphenol-rich extracts can reduce endoplasmic reticulum (ER) stress induced by a high-fat diet (HFD) in skeletal muscle of mice.

Methods

Mice were randomly assigned to four groups receiving during 20 weeks either a standard chow control (CTRL), or a HFD supplemented, or not, with pomegranate (HFD + P) or green tea (HFD + GT) extracts. After the nutritional intervention, mice were killed and gastrocnemius muscles were taken. Proteins and mRNA were measured by Western blot and RT-qPCR, respectively.

Results

Body weight gain and visceral fat were higher in HFD, HFD + P and HFD + GT than in CTRL. The markers of the unfolded protein response BiP, XBP1u, XBP1s and ATF4 were higher only in HFD. In HFD + P and HFD + GT, this increase was not observed except for CHOP, which was elevated in all HFD groups. HFD increased also markers of ubiquitin–proteasome pathway, autophagy and oxidative stress, which were kept low in HFD + P and HFD + GT groups.

Conclusion

Our data provide evidence for a protective effect of pomegranate and green tea extracts against ER stress, oxidative stress and protein degradation induced by HFD in skeletal muscle. They give arguments for a usefulness of these natural nutritional compounds to fight against cellular dysfunctions related to fat excess.

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Abbreviations

ER:

Endoplasmic reticulum

UPR:

Unfolded protein response

HFD:

High-fat diet

EGCG:

Epigallocatechin gallate

P:

Pomegranate

GT:

Green tea

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Acknowledgments

This work was supported by the “Chaire Delhaize” of the “Fondation Louvain” (Belgium). JR is a recipient of a postdoctoral fellowship financed by ELEONOR sprl. HG was a postdoctoral fellow supported by the “Chaire Delhaize”.

Conflict of interest

The authors have declared no conflict of interest.

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Authors and Affiliations

  1. Institute of Neuroscience, Université catholique de Louvain, Place Pierre de Coubertin-1, bte L8.10.01, 1348, Louvain-la-Neuve, Belgium

    Julie Rodriguez, Hélène Gilson, Cécile Jamart, Damien Naslain, Nicolas Pierre & Marc Francaux

  2. Exercise Physiology Research Group, Department of Kinesiology, KU Leuven, Leuven, Belgium

    Louise Deldicque

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  1. Julie Rodriguez
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  2. Hélène Gilson
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  3. Cécile Jamart
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  4. Damien Naslain
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  5. Nicolas Pierre
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  6. Louise Deldicque
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  7. Marc Francaux
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Correspondence to Marc Francaux.

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Rodriguez, J., Gilson, H., Jamart, C. et al. Pomegranate and green tea extracts protect against ER stress induced by a high-fat diet in skeletal muscle of mice. Eur J Nutr 54, 377–389 (2015). https://doi.org/10.1007/s00394-014-0717-9

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  • DOI: https://doi.org/10.1007/s00394-014-0717-9

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