Abstract
In order to identify the expression of coral genes induced by stressors such as suspended red soil, mRNA differential display was examined for the coral Pocillopora damicornis. Apparent differences were observed in the gene expression profiles between control colonies and those treated with local red soil, indicating that red soil affects gene expression in P. damicornis. We cloned nine candidate PCR fragments derived from the differentially expressed genes. One of the clones, pPd9-1, showed a high similarity to a member of the heat-shock protein (HSP) 70 family, suggesting that red soil may cause protein denaturation in the coral. Reverse transcription-mediated PCR showed that the expression of pPd9-1 was also increased by elevated temperature, but not by reduced salinity.
Similar content being viewed by others
References
Afele JC, Tabei Y, Yamada T, Momiyama T, Takaiwa F, Kayano T, Nishimura S, Nishio T (1996) Identification of mRNAs differentially expressed between embryogenic and non-embryogenic cultivars of eggplant during somatic embryogenesis. Jpn Agri Res Q 30:175–179
Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ (1990) Basic local alignment search tool. J Mol Biol 215:403–410
Black NA, Voellmy R, Szmant AM (1995) Heat shock protein induction in Montastraea faveolata and Aiptasia pallida exposed to elevated temperature. Biol Bull 188:234–240
Chomczynski P (1993) A reagent for the single-step simultaneous isolation of RNA, DNA, and proteins from cell and tissue samples. BioTechniques 15:532–536
Choresh O, Ron E, Loya Y (2001) The 60-kDa heat shock protein (HSP60) of the sea anemone Anemonia viridis: a potential early warning system for environmental changes. Mar Biotechnol 3:501–508
Davies JM, Dunne RP, Brown BE (1997) Coral bleaching and elevated sea-water temperature in Milne Bay Province, Papua New Guinea, 1996. Mar Freshwater Res 48:513–516
Fang LS, Huang SP, Lim KL (1997) High temperature induces the synthesis of heat-shock proteins and the elevation of intracellular calcium in the coral Acropora grandis. Coral Reefs 16:127–131
Gates RD, Baghdasarian G, Muscatine L (1992) Temperature stress causes host cell detachment in symbiotic Cnidarians: Implications for coral bleaching. Biol Bull 182:324–332
Gellner K, Praetzel G, Bosch TCG (1992) Cloning and expression of a heat-inducible hsp70 gene in two species of Hydra which differ in their stress response. Eur J Biochem 210:683–691
Hayes RL, King CM (1995) Induction of 70-kD heat shock protein in scleractinian corals by elevated temperature: significance for coral bleaching. Mol Mar Biol Biotechnol 4:36–42
Hodgson G (1990) Tetracycline reduces sedimentation damage to corals. Mar Biol 104:493–496
Hoegh-Guldberg O, Smith GJ (1989) The effect of sudden changes in temperature, light and salinity on the population density and export of zooxanthellae from the reef corals Stylophora pistillata Esper and Seriatopora hystrix Dana. J Exp Mar Biol Ecol 129:279–303
Jokiel PL, Coles SL (1990) Response of Hawaiian and other Indo-Pacific reef corals to elevated temperature. Coral Reefs 8:155–162
Kato M (1987) Mucus-sheet formation and discoloration in the reef-building coral, Porites cylindrica: Effects of altered salinity and temperature. Galaxea 6:1–16
Lindquist S, Craig EA (1988) The heat-shock proteins. Annu Rev Genet 22:631–677
Martin KJ, Kwan C-P, O’Hare MJ, Pardee AB, Sager R (1998) Identification and verification of differential display cDNAs using gene-specific primers and hybridization arrays. BioTechniques 24:1018–1026
Moberg F, Nyström M, Kautsky N, Tedengren M, Jarayabhand P (1997) Effects of reduced salinity on the rates of photosynthesis and respiration in the hermatypic corals Porites lutea and Pocillopora damicornis. Mar Ecol Prog Ser 157:53–59
Momiyama T, Afele JC, Saito T, Kayano T, Tabei Y, Takaiwa F, Takayanagi K, Nishimura S (1995) Differential display identifies developmentally regulated genes during somatic embryogenesis in eggplant ( Solanum menogena L.). Biochem Biophys Res Commun 213:376–382
Mous PJ, Pet-Soede L, Erdmann M, Cesar HSJ, Sadovy Y, Pet JS (2000) Cyanide fishing on Indonesian coral reefs for the live food fish market—What is the problem? SPC Live Reef Fish Information Bull 7:20–26
Muthiga NA, Szmant AM (1987) The effects of salinity stress on the rates of aerobic respiration and photosynthesis in the hermatypic coral Siderastrea siderea. Biol Bull 173:539–551
Nishihira M (1987) Natural and human interference with the coral reef and coastal environments in Okinawa. Galaxea 6:311–321
Rogers CS (1990) Responses of coral reef organisms to sedimentation (review). Mar Ecol Prog Ser 62:185–202
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, New York
Sanders BM (1993) Stress proteins in aquatic organisms: an environmental perspective. Crit Rev Toxicol 23:49–75
Sanders BM (1994) Stress proteins as molecular chaperones: implications for toxicology. Environ Health Perspect 102:538–540
Sharp VA, Brown BE, Miller D (1997) Heat shock protein (hsp70) expression in the tropical reef coral Goniopora djiboutiensis. J Therm Biol 22:11–19
Sharp VA, Miller D, Bythell JC, Brown BE (1994) Expression of low molecular weight HSP 70 related polypeptides from the symbiotic sea anemone Anemonia viridis Forskall in response to heat shock. J Exp Mar Biol Ecol 179:179–193
Ting J, Lee AS (1988) Human gene encoding the 78,000-dalton glucose-regulated protein and its pseudogene: structure, conservation, and regulation. DNA 7:275–286
Wiens M, Ammar MSA, Nawar AH, Kozoil C, Hassanein HMA, Eisinger M, Müller IM, Müller WEG (2000) Induction of heat-shock (stress) protein gene expression by selected natural and anthropogenic disturbances in the octocoral Dendronephthya klunzingeri. J Exp Mar Biol Ecol 245:265–276
Yamada Y, Motomura S, Matsuzaka Y, Kato Y (1972) Soils of Ishigaki, Miyako and Yonaguni Islands (in Japanese with English summary). Bull Nat Inst Agri Sci, Series B (Soils and Fertilizers) 24:265–365
Yamasu T, Mizofuchi S (1989) Effects of synthetic, neutral detergent and red clay on short-term measurements of O2 production in an Okinawan reef coral. Galaxea 8:127–142
Yamazato K (1987) Effects of deposition and suspension of inorganic particulate matter on the reef building corals in Okinawa, Japan. Galaxea 6:289–309
Zhao S, Ooi SL, Pardee AB (1995) New primer strategy improves precision of differential display. BioTechniques 18:842–850
Acknowledgements
The authors thank Drs. M. Katoh, O. Abe, Y. Takada, C. P. Norman, and anonymous reviewers for their helpful comments on the manuscript. Ms. R. Ooi assisted with molecular technology. This work was supported by the Fisheries Agency of Japan. This is contribution no. 646 of the Seikai National Fisheries Research Institute.
Author information
Authors and Affiliations
Corresponding author
Additional information
Communicated by H.R. Lasker
Rights and permissions
About this article
Cite this article
Hashimoto, K., Shibuno, T., Murayama-Kayano, E. et al. Isolation and characterization of stress-responsive genes from the scleractinian coral Pocillopora damicornis. Coral Reefs 23, 485–491 (2004). https://doi.org/10.1007/s00338-004-0410-1
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00338-004-0410-1