Article

Mammalian Genome

, Volume 23, Issue 9, pp 580-586

Open Access This content is freely available online to anyone, anywhere at any time.

The mammalian gene function resource: the international knockout mouse consortium

  • Allan BradleyAffiliated withThe Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus Email author 
  • , Konstantinos AnastassiadisAffiliated withBiotechnology Center (BIOTEC) of the Technische Universität Dresden
  • , Abdelkader AyadiAffiliated withInstitut Clinique de la Souris and Institut de Génétique et de Biologie Moléculaire et Cellulaire
  • , James F. BatteyAffiliated withNational Institute on Deafness and Other Communication Disorders (NIH)
  • , Cindy BellAffiliated withGenome Canada
  • , Marie-Christine BirlingAffiliated withInstitut Clinique de la Souris and Institut de Génétique et de Biologie Moléculaire et Cellulaire
  • , Joanna BottomleyAffiliated withThe Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus
  • , Steve D. BrownAffiliated withMammalian Genetics Unit, MRC Harwell
  • , Antje BürgerAffiliated withInstitute of Developmental Genetics, Helmholtz Zentrum München, Technische Universität München
    • , Carol J. BultAffiliated withThe Jackson Laboratory
    • , Wendy BushellAffiliated withThe Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus
    • , Francis S. CollinsAffiliated withNational Institutes of Health
    • , Christian DesaintesAffiliated withThe Wellcome Trust Sanger Institute, Wellcome Trust Genome CampusInfectious Diseases and Public Health, European Commission, DG Research & Innovation
    • , Brendan DoeAffiliated withThe Wellcome Trust Sanger Institute, Wellcome Trust Genome CampusIstituto di Biologia Cellulare, Consiglio Nazionale delle Ricerche (CNR), Monterotondo-Scalo
    • , Aris EconomidesAffiliated withThe Wellcome Trust Sanger Institute, Wellcome Trust Genome CampusVelocigene Division, Regeneron Pharmaceuticals Inc.
    • , Janan T. EppigAffiliated withThe Jackson Laboratory
    • , Richard H. FinnellAffiliated withThe Wellcome Trust Sanger Institute, Wellcome Trust Genome CampusThe Texas A&M Institute for Genomic MedicineUniversity of Texas at Austin
    • , Colin FletcherAffiliated withThe Wellcome Trust Sanger Institute, Wellcome Trust Genome CampusNational Institutes of Health
    • , Martin FrayAffiliated withMammalian Genetics Unit, MRC Harwell
    • , David FrendeweyAffiliated withThe Wellcome Trust Sanger Institute, Wellcome Trust Genome CampusVelocigene Division, Regeneron Pharmaceuticals Inc.
    • , Roland H. FriedelAffiliated withInstitut Clinique de la Souris and Institut de Génétique et de Biologie Moléculaire et CellulaireInstitute of Developmental Genetics, Helmholtz Zentrum München, Technische Universität MünchenIcahn Medical Institute, The Mount Sinai Hospital
    • , Frank G. GrosveldAffiliated withThe Wellcome Trust Sanger Institute, Wellcome Trust Genome CampusDepartment of Cell Biology, Center of Biomedical Genetics, Erasmus University Medical Center
    • , Jens HansenAffiliated withInstitute of Developmental Genetics, Helmholtz Zentrum München, Technische Universität München
    • , Yann HéraultAffiliated withInstitut Clinique de la Souris and Institut de Génétique et de Biologie Moléculaire et Cellulaire
    • , Geoffrey HicksAffiliated withThe Wellcome Trust Sanger Institute, Wellcome Trust Genome CampusManitoba Institute of Cell Biology, University of Manitoba
    • , Andreas HörleinAffiliated withInstitute of Developmental Genetics, Helmholtz Zentrum München, Technische Universität München
    • , Richard HoughtonAffiliated withThe Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus
    • , Martin Hrabé de AngelisAffiliated withThe Wellcome Trust Sanger Institute, Wellcome Trust Genome CampusInstitute of Experimental Genetics, Helmholtz Zentrum München
    • , Danny HuylebroeckAffiliated withThe Wellcome Trust Sanger Institute, Wellcome Trust Genome CampusDepartment of Development and Regeneration, Faculty of Medicine, University of Leuven (KU Leuven)
    • , Vivek IyerAffiliated withThe Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus
    • , Pieter J. de JongAffiliated withBiotechnology Center (BIOTEC) of the Technische Universität DresdenChildren’s Hospital Oakland Research Institute (CHORI)
    • , James A. KadinAffiliated withThe Jackson Laboratory
    • , Cornelia KaloffAffiliated withInstitute of Developmental Genetics, Helmholtz Zentrum München, Technische Universität München
    • , Karen KennedyAffiliated withThe Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus
    • , Manousos KoutsourakisAffiliated withThe Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus
    • , K. C. Kent LloydAffiliated withBiotechnology Center (BIOTEC) of the Technische Universität DresdenMouse Biology Program, School of Veterinary Medicine, University of California
    • , Susan MarschallAffiliated withThe Wellcome Trust Sanger Institute, Wellcome Trust Genome CampusInstitute of Experimental Genetics, Helmholtz Zentrum München
    • , Jeremy MasonAffiliated withThe Jackson Laboratory
    • , Colin McKerlieAffiliated withBiotechnology Center (BIOTEC) of the Technische Universität DresdenResearch Institute, The Hospital for Sick Children, SickKids Foundation
    • , Michael P. McLeodAffiliated withThe Wellcome Trust Sanger Institute, Wellcome Trust Genome CampusThe Texas A&M Institute for Genomic Medicine
    • , Harald von MelchnerAffiliated withBiotechnology Center (BIOTEC) of the Technische Universität DresdenDepartment of Molecular Haematology, University of Frankfurt Medical School
    • , Mark MooreAffiliated withThe Wellcome Trust Sanger Institute, Wellcome Trust Genome CampusNational Institutes of Health
    • , Alejandro O. MujicaAffiliated withThe Wellcome Trust Sanger Institute, Wellcome Trust Genome CampusVelocigene Division, Regeneron Pharmaceuticals Inc.
    • , Andras NagyAffiliated withBiotechnology Center (BIOTEC) of the Technische Universität DresdenSamuel Lunenfeld Research Institute, Mount Sinai Hospital
    • , Mikhail NefedovAffiliated withBiotechnology Center (BIOTEC) of the Technische Universität DresdenChildren’s Hospital Oakland Research Institute (CHORI)
    • , Lauryl M. NutterAffiliated withBiotechnology Center (BIOTEC) of the Technische Universität DresdenResearch Institute, The Hospital for Sick Children, SickKids Foundation
    • , Guillaume PavlovicAffiliated withInstitut Clinique de la Souris and Institut de Génétique et de Biologie Moléculaire et Cellulaire
    • , Jane L. PetersonAffiliated withThe Wellcome Trust Sanger Institute, Wellcome Trust Genome CampusNational Institutes of Health
    • , Jonathan PollockAffiliated withBiotechnology Center (BIOTEC) of the Technische Universität DresdenDivision of Basic Neuroscience and Research, National Institute of Drug Abuse (NIDA)
    • , Ramiro Ramirez-SolisAffiliated withThe Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus
    • , Derrick E. RancourtAffiliated withBiotechnology Center (BIOTEC) of the Technische Universität DresdenDepartment of Biochemistry and Molecular Biology, University of Calgary
    • , Marcello RaspaAffiliated withThe Wellcome Trust Sanger Institute, Wellcome Trust Genome CampusIstituto di Biologia Cellulare, Consiglio Nazionale delle Ricerche (CNR), Monterotondo-Scalo
    • , Jacques E. RemacleAffiliated withThe Wellcome Trust Sanger Institute, Wellcome Trust Genome CampusInfectious Diseases and Public Health, European Commission, DG Research & Innovation
    • , Martin RingwaldAffiliated withThe Jackson Laboratory
    • , Barry RosenAffiliated withThe Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus
    • , Nadia RosenthalAffiliated withBiotechnology Center (BIOTEC) of the Technische Universität DresdenEuropean Molecular Biology Laboratory (EMBL)
    • , Janet RossantAffiliated withBiotechnology Center (BIOTEC) of the Technische Universität DresdenResearch Institute, The Hospital for Sick Children, SickKids Foundation
    • , Patricia Ruiz NoppingerAffiliated withBiotechnology Center (BIOTEC) of the Technische Universität DresdenCentre for Cardiovascular Research, Department of Vertebrate Genomics, Charité
    • , Ed RyderAffiliated withThe Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus
    • , Joel Zupicich SchickAffiliated withInstitute of Developmental Genetics, Helmholtz Zentrum München, Technische Universität München
    • , Frank SchnütgenAffiliated withBiotechnology Center (BIOTEC) of the Technische Universität DresdenDepartment of Molecular Haematology, University of Frankfurt Medical School
    • , Paul SchofieldAffiliated withInstitut Clinique de la Souris and Institut de Génétique et de Biologie Moléculaire et CellulaireDepartment of Physiology, Development and Neuroscience, University of Cambridge
    • , Claudia SeisenbergerAffiliated withInstitute of Developmental Genetics, Helmholtz Zentrum München, Technische Universität München
    • , Mohammed SelloumAffiliated withInstitut Clinique de la Souris and Institut de Génétique et de Biologie Moléculaire et Cellulaire
    • , Elizabeth M. SimpsonAffiliated withInstitut Clinique de la Souris and Institut de Génétique et de Biologie Moléculaire et CellulaireDepartment of Medical Genetics, Centre for Molecular Medicine and Therapeutics at the Child & Family Research Institute, University of British Columbia
    • , William C. SkarnesAffiliated withThe Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus
    • , Damian SmedleyAffiliated withThe Wellcome Trust Sanger Institute, Wellcome Trust Genome CampusInstitut Clinique de la Souris and Institut de Génétique et de Biologie Moléculaire et CellulaireEuropean Bioinformatics Institute (EBI)
    • , William L. StanfordAffiliated withInstitut Clinique de la Souris and Institut de Génétique et de Biologie Moléculaire et CellulaireThe Ottawa Hospital Research Institute
    • , A. Francis StewartAffiliated withBiotechnology Center (BIOTEC) of the Technische Universität Dresden
    • , Kevin StoneAffiliated withThe Jackson Laboratory
    • , Kate SwanAffiliated withGenome Canada
    • , Hamsa TadepallyAffiliated withThe Jackson Laboratory
    • , Lydia TeboulAffiliated withMammalian Genetics Unit, MRC Harwell
    • , Glauco P. Tocchini-ValentiniAffiliated withThe Wellcome Trust Sanger Institute, Wellcome Trust Genome CampusIstituto di Biologia Cellulare, Consiglio Nazionale delle Ricerche (CNR), Monterotondo-Scalo
    • , David ValenzuelaAffiliated withThe Wellcome Trust Sanger Institute, Wellcome Trust Genome CampusVelocigene Division, Regeneron Pharmaceuticals Inc.
    • , Anthony P. WestAffiliated withThe Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus
    • , Ken-ichi YamamuraAffiliated withInstitut Clinique de la Souris and Institut de Génétique et de Biologie Moléculaire et CellulaireDivision of Developmental Genetics, Center for Animal Resources and Development, Institute of Resource Development and Analysis, Kumamoto University
    • , Yuko YoshinagaAffiliated withBiotechnology Center (BIOTEC) of the Technische Universität DresdenChildren’s Hospital Oakland Research Institute (CHORI)
    • , Wolfgang WurstAffiliated withInstitut Clinique de la Souris and Institut de Génétique et de Biologie Moléculaire et CellulaireInstitute of Developmental Genetics, Helmholtz Zentrum München, Technische Universität MünchenMax-Planck-Institute of PsychiatryDeutsches Zentrum fuer Neurodegenerative Erkrankungen e.V. (DZNE) Site Munich Email author 

Abstract

In 2007, the International Knockout Mouse Consortium (IKMC) made the ambitious promise to generate mutations in virtually every protein-coding gene of the mouse genome in a concerted worldwide action. Now, 5 years later, the IKMC members have developed high-throughput gene trapping and, in particular, gene-targeting pipelines and generated more than 17,400 mutant murine embryonic stem (ES) cell clones and more than 1,700 mutant mouse strains, most of them conditional. A common IKMC web portal (www.​knockoutmouse.​org) has been established, allowing easy access to this unparalleled biological resource. The IKMC materials considerably enhance functional gene annotation of the mammalian genome and will have a major impact on future biomedical research.