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Wound-induced expression of DEFECTIVE IN ANTHER DEHISCENCE1 and DAD1-like lipase genes is mediated by both CORONATINE INSENSITIVE1-dependent and independent pathways in Arabidopsis thaliana

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Abstract

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Endogenous JA production is not necessary for wound-induced expression of JA-biosynthetic lipase genes such as DAD1 in Arabidopsis. However, the JA-Ile receptor COI1 is often required for their JA-independent induction.

Abstract

Wounding is a serious event in plants that may result from insect feeding and increase the risk of pathogen infection. Wounded plants produce high amounts of jasmonic acid (JA), which triggers the expression of insect and pathogen resistance genes. We focused on the transcriptional regulation of DEFECTIVE IN ANTHER DEHISCENCE1 and six of its homologs including DONGLE (DGL) in Arabidopsis, which encode lipases involved in JA biosynthesis. Plants constitutively expressing DAD1 accumulated a higher amount of JA than control plants after wounding, indicating that the expression of these lipase genes contributes to determining JA levels. We found that the expression of DAD1, DGL, and other DAD1-LIKE LIPASE (DALL) genes is induced upon wounding. Some DALLs were also expressed in unwounded leaves. Further experiments using JA-biosynthetic and JA-response mutants revealed that the wound induction of these genes is regulated by several distinct pathways. DAD1 and most of its homologs other than DALL4 were fully induced without relying on endogenous JA-Ile production and were only partly affected by JA deficiency, indicating that positive feedback by JA is not necessary for induction of these genes. However, DAD1 and DGL required CORONATINE INSENSITIVE1 (COI1) for their expression, suggesting that a molecule other than JA might act as a regulator of COI1. Wound induction of DALL1, DALL2, and DALL3 did not require COI1. This differential regulation of DAD1 and its homologs might explain their functions at different time points after wounding.

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Acknowledgments

The mutant coi1-51 was identified and provided by Dr. Takashi Araki (Kyoto University). We thank Dr. Yusuke Jikumaru and Ms. Tomoe Nose of RIKEN for JA measurement, and Dr. Kenichiro Maeo, Dr. Ryo Tabata, Dr. Tomoko Niwa, and Ms. Kanako Nakano of Nagoya University for materials, comments, and helpful discussion. This work was financed by the Japan Society for the Promotion of Science (JSPS) (Grant-in-aid for Scientific Research no. 21•09098 to I.R. and S.I.). I.R. was a recipient of the Postdoctoral Fellowships for Foreign Researchers from JSPS.

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  1. Kenzo Nakamura

    Present address: College of Bioscience and Biotechnology, Chubu University, Kasugai, 487-8501, Japan

Authors and Affiliations

  1. Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, 464-8601, Japan

    Izabela Ruduś, Haruka Terai, Hisae Kojima, Kazuki Hattori, Yuka Nishimori, Hironaka Tsukagoshi, Kenzo Nakamura & Sumie Ishiguro

  2. Department of Plant Physiology and Genetic Engineering, Szczecin University, Wąska 13, 71 415, Szczecin, Poland

    Izabela Ruduś & Jan Kępczyński

  3. RIKEN Center for Sustainable Resource Science, Yokohama, 230-0045, Japan

    Takafumi Shimizu, Yuji Kamiya & Mitsunori Seo

  4. Precursory Research for Embryonic Science and Technology, Japan Science and Technology Agency, Kawaguchi, 332-0022, Japan

    Hironaka Tsukagoshi

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  1. Izabela Ruduś
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Correspondence to Sumie Ishiguro.

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Communicated by F. Sato.

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Ruduś, I., Terai, H., Shimizu, T. et al. Wound-induced expression of DEFECTIVE IN ANTHER DEHISCENCE1 and DAD1-like lipase genes is mediated by both CORONATINE INSENSITIVE1-dependent and independent pathways in Arabidopsis thaliana . Plant Cell Rep 33, 849–860 (2014). https://doi.org/10.1007/s00299-013-1561-8

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