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Retention Sites for Xylella fastidiosa in Four Sharpshooter Vectors (Hemiptera: Cicadellidae) Analyzed by Scanning Electron Microscopy

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Abstract

Xylella fastidiosa is a xylem-limited bacterium that causes citrus variegated chlorosis (CVC), Pierce’s disease of grapevine, and leaf scald of coffee and plum and many other plant species. This pathogen is vectored by sharpshooter leafhoppers (Hemiptera: Cicadellidae: Cicadellinae) and resides in the insect foregut. Scanning electron microscopy was used to determine the retention sites of X. fastidiosa for the most common vector species in Brazilian citrus groves, Acrogonia citrina, Bucephalogonia xanthophis, Dilobopterus costalimai, and Oncometopia facialis. After a 48-h acquisition access period on infected citrus or plum, adult sharpshooters were kept on healthy citrus seedlings for an incubation period of 2 weeks to allow for bacterial multiplication. Then the vector heads were incubated for 24 h in a fixative and transferred into a cryoprotector liquid. Bacterial rod cells exhibiting similar X. fastidiosa morphology were found laterally attached to different regions inside the cibarial pump chamber (longitudinal groove, lateral surface, cibarial diaphragm and apodemal groove) of A. citrina, O. facialis, and D. costalimai, and polarly attached to the precibarium channel of O. facialis. Polymerase chain reactions of vector’s heads were positive for the presence of X. fastidiosa. No X. fastidiosa-like cells were detected in B. xanthophis. A different type of rod-shaped bacterium was found on B. xanthophis cibarium chamber and images suggest that the cibarium wall was degraded/digested by these bacteria. Colonization patterns of X. fastidiosa in their vectors are fundamental aspects to be explored toward understanding acquisition, adhesion, and transmission mechanisms for development of X. fastidiosa control strategies.

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Acknowledgments

The authors acknowledge Dr. Elliot W. Kitajima for his assistance during the development of this study. We are grateful to Dr. Maria L. Ishida and Brent Brodbeck for critically reviewing the manuscript. This work was partially funded by the California Department of Food and Agriculture and a U.S. Department of Agriculture grant administered by the University of California, Davis.

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Authors and Affiliations

  1. Departamento de Fitopatologia, Universidade Federal de Lavras, Caixa Postal 3037, 37200-000, Lavras, MG, Brasil

    Eduardo Alves

  2. Thermo Scientific, 5225 Verona Road, Madison, WI, USA

    Breno Leite

  3. Faculdade de Ciências Agrárias, Centro Universitário de Patos de Minas, FACIAGRA-UNIPAM, Caixa Postal 485, 38702-054, Patos de Minas, MG, Brasil

    Rosangela C. Marucci

  4. Departamento de Entomologia, Fitopatologia E Zoologia Agrícola, ESALQ-USP, Caixa Postal 9, 13418-900, Piracicaba, SP, Brasil

    Sérgio F. Pascholati & João R. S. Lopes

  5. NFREC-IFAS, University of Florida, 155 Research Road, Quincy, FL, 32351, USA

    Peter C. Andersen

Authors
  1. Eduardo Alves
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  2. Breno Leite
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  3. Rosangela C. Marucci
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  4. Sérgio F. Pascholati
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  5. João R. S. Lopes
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  6. Peter C. Andersen
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Correspondence to Peter C. Andersen.

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Alves, E., Leite, B., Marucci, R.C. et al. Retention Sites for Xylella fastidiosa in Four Sharpshooter Vectors (Hemiptera: Cicadellidae) Analyzed by Scanning Electron Microscopy. Curr Microbiol 56, 531–538 (2008). https://doi.org/10.1007/s00284-008-9119-7

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  • DOI: https://doi.org/10.1007/s00284-008-9119-7

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