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A strategy for enhancing the engraftment of human hematopoietic stem cells in NOD/SCID mice

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Abstract

To overcome the limitations of allogeneic hematopoietic stem cell transplantation (HSCT), we conducted a study to identify a strategy for enhancing hematopoietic stem cell (HSC) engraftment during HSCT. Co-transplantation experiments with mesenchymal stem cells (MSCs) derived from adult human tissues including bone marrow (BM), adipose tissue (AT), and umbilical cord blood (CB) were conducted. We showed that AT-MSCs and CB-MSCs enhanced the engraftment of HSCs as effectively as BM-MSCs in NOD/SCID mice, suggesting that AT-MSCs and CB-MSCs can be used as alternative stem cell sources for enhancing the engraftment and homing of HSCs. CB-MSCs derived from different donors showed different degrees of efficacy in enhancing the engraftment of HSCs. The most effective CB-MSCs showed higher proliferation rates and secreted more MCP-1, RANTES, EGF, and VEGF. Our results suggest that AT-MSCs and CB-MSCs could be alternative stem cell sources for co-transplantation in HSCT. Furthermore, in terms of MSCs’ heterogeneity, characteristics of each population of MSCs are considerable factors for selecting MSCs suitable for co-transplantation with HSC.

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Acknowledgments

This study was supported by a grant of the Korean Health Technology R&D Project, Ministry for Health, Welfare and Family affairs, Republic of Korea (project no: A090729).

Conflicts of interest

The authors declare no conflicts of interest.

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Authors and Affiliations

  1. Department of Pediatrics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, South Korea

    Soo Hyun Lee, Dae Seong Kim, Myoung Woo Lee, Yoo Hun Noh, In Keun Jang, Hee Won Chueh, Meong Hi Son, Hye Lim Jung, Keon Hee Yoo, Ki Woong Sung & Hong Hoe Koo

  2. Department of Physiology and Pharmacology, College of Medicine, The University of Toledo, Ohio, USA

    Dong Hyun Kim

  3. Transplantation Research Center, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, South Korea

    Heung-Mo Yang & Sung-Joo Kim

  4. Biomedical Research Institute, R&D center, MEDIPOST CO., Ltd, Seoul, South Korea

    Soo Jin Choi, Wonil Oh & Yoon Sun Yang

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  1. Soo Hyun Lee
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  2. Dae Seong Kim
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  3. Myoung Woo Lee
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Corresponding authors

Correspondence to Myoung Woo Lee or Hong Hoe Koo.

Additional information

Soo Hyun Lee and Dae Seong Kim contributed equally to this work.

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Lee, S.H., Kim, D.S., Lee, M.W. et al. A strategy for enhancing the engraftment of human hematopoietic stem cells in NOD/SCID mice. Ann Hematol 92, 1595–1602 (2013). https://doi.org/10.1007/s00277-013-1830-1

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  • DOI: https://doi.org/10.1007/s00277-013-1830-1

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