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Diversity and evolution of the highly polymorphic tandem repeat LEI0258 in the chicken MHC-B region

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Abstract

The chicken major histocompatibility complex (MHC) is located on the microchromosome 16 and is described as the most variable region in the genome. The genes of the MHC play a central role in the immune system. Particularly, genes encoding proteins involved in the antigen presentation to T cells. Therefore, describing the genetic polymorphism of this region is crucial in understanding host–pathogen interactions. The tandem repeat LEI0258 is located within the core area of the B region of the chicken MHC (MHC-B region) and its genotypes correlate with serology. This marker was used to provide a picture of the worldwide diversity of the chicken MHC-B region and to categorize chicken MHC haplotypes. More than 1,600 animals from 80 different populations or lines of chickens from Africa, Asia, and Europe, including wild fowl species, were genotyped at the LEI0258 locus. Fifty novel alleles were described after sequencing. The resulting 79 alleles were classified into 12 clusters, based on the SNPs and indels found within the sequences flanking the repeats. Furthermore, hypotheses were formulated on the evolutionary dynamics of the region. This study constitutes the largest variability report for the chicken MHC and establishes a framework for future diversity or association studies.

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Acknowledgments

The authors thank Anne-Marie Chaussé (INRA, Nouzilly, France), Xavier Rognon (INRA, Jouy en Josas, France), Yannick Roman (Zoo de Clères, Clères, France), and Cheng Yu-Shin (Livestock Research Institute, Sinhua, Taiwan) for their help collecting DNA samples. They also thank Janet Fulton (Hy-Line International, Dallas Center, USA) for the helpful comments and discussion. This work was supported by a studentship awarded by the French ministry of Higher Education and Research.

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Authors and Affiliations

  1. Génétique Animale et Biologie Intégrative, INRA, AgroParisTech, UMR 1313, Jouy-en-Josas, France

    Olympe Chazara, Nicolas Bruneau, Marie-Hélène Pinard-van der Laan, Michèle Tixier-Boichard & Bertrand Bed’Hom

  2. Department of Pathology, University of Cambridge and Centre for Trophoblast Research, Tennis Court Road, CB2 1QP, Cambridge, UK

    Olympe Chazara

  3. Institute of Statistical Science, Academia Sinica, Taipei, Taiwan

    Chi-Sheng Chang

  4. Institut Agronomique et Vétérinaire Hassan II, DPBA, Rabat Institute, Rabat, Morocco

    Khalid Benabdeljelil

  5. Institut de Recherche Agricole pour le Développement, Bamenda, Cameroon

    Jean-Claude Fotsa

  6. Department of Animal Science, University of Ghana, Legon, Ghana

    Boniface B. Kayang & Richard Osei-Amponsah

  7. Centre Nationale de Recherche Agronomique, Université de Cocody, Abidjan, Ivory Coast

    N’Goran E. Loukou & Valentine Yapi-Gnaore

  8. Ecole Polytechnique d’Abomey-Calavi, Université d’Abomey-Calavi, Cotonou, Benin

    Issaka A. K. Youssao

  9. Department of Animal Science, National Chung-Hsing University, Taichung, Taiwan

    Chih-Feng Chen

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  1. Olympe Chazara
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Correspondence to Olympe Chazara.

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Chazara, O., Chang, CS., Bruneau, N. et al. Diversity and evolution of the highly polymorphic tandem repeat LEI0258 in the chicken MHC-B region. Immunogenetics 65, 447–459 (2013). https://doi.org/10.1007/s00251-013-0697-6

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  • DOI: https://doi.org/10.1007/s00251-013-0697-6

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