Abstract
This study experimentally demonstrates how application of an external physical stress onto the skin membrane affects the permeation of penetrating molecules. As a proxy of active compounds, in this study, a series of fluorescence probe molecules were utilized. We observed that skin permeation could be enhanced by imparting vertical strokes from a tapping head consisting of projections onto the skin. This was confirmed with consistency from in vitro and in vivo transdermal permeation studies. After an effective physical stress was applied to the skin, the permeation depth of probe molecules remarkably increased, which was comparable to the case of topical treatment. This seems to arise from temporal disordering of the stratum corneum layer in response to the applied physical stress.
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References
Baxter J, Mitragotri S (2005) Jet-induced skin puncture and its impact on needle-free jet injections: experimental studies and a predictive model. J Control Release 106:361–373
Borgia SL (2005) Lipid nanoparticles for skin penetration enhancement—correlation to drug localization within the particle matrix as determined by fluorescence and parelectric spectroscopy. J Control Release 110:151–163
Brown MB, Martin GP, Jones SA, Akomeah FK (2006) Dermal and transdermal drug delivery systems: current and future prospects. Drug Deliv 13:175–187
Chan ECY, Tan WL, Ho PC, Fang LJ (2005) Modeling Caco-2 permeability of drugs using immobilized artificial membrane chromatography and physicochemical descriptors. J Chromatogr A 1072:159–168
Doukas AG, Kollias N (2004) Transdermal drug delivery with a pressure wave. Adv Drug Deliv Rev 56:559–579
Eccles AS, Aberd MB (1896) The practice of massage. Nature 54:411–412
Elias PM (2005) Stratum corneum defensive functions: an integrated view. J Investig Dermatol 125:183–200
Evans E, Yeung A (1994) Hidden dynamics in rapid changes of bilayer shape. Chem Phys Lipids 73:39–56
Kalia YN, Naik A, Garrison J, Guy RH (2004) Iontophoretic drug delivery. Adv Drug Deliv Rev 56:619–658
Karande P, Mitragotri S (2003) Dependence of skin permeability on contact area. Pharm Res 20:257–263
Lavon I, Kost J (2004) Ultrasound and transdermal drug delivery. Drug Discov Today 9:670–676
Michaels AS, Chandrasekaran SK, Shaw JE (1975) Drug permeation through human skin: theory and in vitro experimental measurement. AlChE J 21:985–996
Mikszta JA, Alarcon JB, Brittingham JM, Sutter DE, Pettis RJ, Harvey NG (2002) Improved genetic immunization via micromechanical disruption of skin-barrier function and targeted epidermal delivery. Nat Med 8:415–419
Mitragotri S (2003) Modeling skin permeability to hydrophilic and hydrophobic solutes based on four permeation pathways. J Control Release 86:69–92
Mitragotri S (2006) Current status and future prospects of needle-free liquid jet injectors. Nat Rev Drug Discov 5:543–548
Mortimer PS, Simmonds R, Rezvani M, Robbins M, Hopewell JW, Ryan TJ (1990) The measurement of skin lymph flow by isotope clearance—reliability, reproducibility, injection dynamics, and the effect of massage. J Investig Dermatol 95:677–682
Pedersen L, Jemec GBE (2006) Mechanical properties and barrier function of healthy human skin. Acta Derm Venereol 86:308–311
Prausnitz MR (2004) Microneedles for transdermal drug delivery. Adv Drug Deliv Rev 56:581–587
Prausnitz MR, Mitragotri S, Langer R (2004) Current status and future potential of transdermal drug delivery. Nat Rev Drug Discov 2:115–124
Raphael RM, Waugh RE, Svetina S, Zeks B (2001) Fractional occurrence of defects in membranes and mechanically driven interleaflet phospholipid transport. Phys Rev E 64:051913
Sakurai H, Takahashi Y, Machida Y (2005) Influence of low-frequency massage device on transdermal absorption of ionic materials. Int J Pharm 305:112–121
Treffel P, Panisset F, Humbert P, Remoussenard O, Bechtel Y, Agache P (1993) Effect of pressure on in vitro percutaneous absorption of caffeine. Acta Derm Venereol 73:200–202
Acknowledgments
This work was supported by the research fund of Hanyang University (HY-2011-N). This work is also supported by the National Research Foundation (NRF) Grant funded by the Korean Government (MEST) (2011-0014663).
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Lee, H., Kim, J.W. Diffusion Behaviors of Fluorescence Probe Molecules Through the Stratum Corneum Layer Under Physical Stress. J Membrane Biol 246, 263–269 (2013). https://doi.org/10.1007/s00232-013-9527-x
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DOI: https://doi.org/10.1007/s00232-013-9527-x