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SERS analysis of serum for detection of early and locally advanced breast cancer

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Abstract

In this contribution, we investigated whether surface-enhanced Raman scattering (SERS) of serum can be a candidate method for detecting “luminal A” breast cancer (BC) at different stages. We selected three groups of participants aged over 50 years: 20 healthy women, 20 women with early localized small BC, and 20 women affected by BC with lymph node involvement. SERS revealed clear spectral differences between these three groups. A predictive model using principal component analysis (PCA) and linear discriminant analysis (LDA) was developed based on spectral data, and its performance was estimated with cross-validation. PCA-LDA of SERS spectra could distinguish healthy from BC subjects (sensitivity, 92 %; specificity, 85 %), as well as subjects with BC at different stages, with a promising diagnostic performance (sensitivity and specificity, ≥80 %; overall accuracy, 84 %). Our data suggest that SERS spectroscopy of serum, combined with multivariate data analysis, represents a minimally invasive, easy to use, and fast approach to discriminate healthy from BC subjects and even to distinguish BC at different clinical stages.

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Acknowledgments

AB would like to thank Dr. Claudia Beleites for sharing her ideas on LDA applied to vibrational spectra.

Author information

Authors and Affiliations

  1. CRO-Biobank, CRO Aviano National Cancer Institute, Via Franco Gallini 2, 33081, Aviano, PN, Italy

    Silvia Cervo & Agostino Steffan

  2. Clinical Cancer Pathology, CRO Aviano National Cancer Institute, Via Franco Gallini 2, 33081, Aviano, PN, Italy

    Silvia Cervo & Agostino Steffan

  3. Division of Experimental Oncology 2, CRO Aviano National Cancer Institute, Via Franco Gallini 2, 33081, Aviano, PN, Italy

    Elena Mansutti, Riccardo Spizzo & Alfonso Colombatti

  4. Dept. of Engineering and Architecture, University of Trieste, Via Valerio 6a, 34127, Trieste, TS, Italy

    Elena Mansutti, Greta Del Mistro, Valter Sergo & Alois Bonifacio

  5. Centre of Excellence MAT, Dept. of Medical and Biological Science, University of Udine, P.le M. Kolbe 4, 33010, Udine, Italy

    Alfonso Colombatti

Authors
  1. Silvia Cervo
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  2. Elena Mansutti
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Correspondence to Alois Bonifacio.

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Cervo, S., Mansutti, E., Del Mistro, G. et al. SERS analysis of serum for detection of early and locally advanced breast cancer. Anal Bioanal Chem 407, 7503–7509 (2015). https://doi.org/10.1007/s00216-015-8923-8

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  • DOI: https://doi.org/10.1007/s00216-015-8923-8

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