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Choosing between RT-qPCR and RNA-seq: a back-of-the-envelope estimate towards the definition of the break-even-point

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Fig. 1
Fig. 2

Abbreviations

qPCR:

Quantitative real-time polymerase chain reaction

RT-qPCR:

Reverse transcription quantitative real-time polymerase chain reaction

RNA-seq:

RNA sequencing

RT-PCR:

Reverse transcription polymerase chain reaction

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Acknowledgments

The authors are grateful to B. Ruperti (University of Padua, Italy) for critical reading of the manuscript. A.N. was supported by University of Padua and the Agricultural Research Council (CRA), Italy.

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Authors and Affiliations

  1. Department of Agronomy, Food, Natural Resources, Animals and Environment, University of Padua, Viale dell’Università 16, 35020, Legnaro, PD, Italy

    Alberto Nonis

  2. Viticultural Research Center (CRA-VIT), Agricultural Research Council (CRA), Via XXVIII Aprile 26, 31015, Conegliano, TV, Italy

    Alberto Nonis & Barbara De Nardi

  3. University Centre of Statistics for Biomedical Sciences, Vita-Salute San Raffaele University, Via Olgettina 58, 20132, Milan, MI, Italy

    Alessandro Nonis

Authors
  1. Alberto Nonis
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  2. Barbara De Nardi
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  3. Alessandro Nonis
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Correspondence to Alberto Nonis.

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Nonis, A., De Nardi, B. & Nonis, A. Choosing between RT-qPCR and RNA-seq: a back-of-the-envelope estimate towards the definition of the break-even-point. Anal Bioanal Chem 406, 3533–3536 (2014). https://doi.org/10.1007/s00216-014-7687-x

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