Abstract
We investigated the radical scavenging effect and antioxidation property of polypeptide extracted fromChlamys farreri (PCF)in vitro using chemiluminescence and electron spin resonance (ESR) methods. We examined the scavenging effects of PCF on superoxide anions (O −2 ), hydroxyl radicals (OH·), peroxynitrite (ONOO−) and the inhibiting capacity of PCF on peroxidation of linoleic acid. Our experiment suggested that PCF could scavenge oxygen free radicals including superoxide anions (O −2 ) (IC50=0.3 mg/ml), hydroxyl radicals (OH·) (IC50=0.2 μg/ml) generated from the reaction systems and effectively inhibit the oxidative activity of ONOO− (IC50=0.2 mg/ml). At 1.25 mg/ml of PCF, the inhibition ratio on lipid peroxidation of linoleic acid was 43%. The scavenging effect of PCF on O −2 , OH· and ONOO− free radicals were stronger than those of vitamin C but less on lipid peroxidation of linoleic acid. Thus PCF could scavenge free radicals and inhibit the peroxidation of linoleic acidin vitro. It is an antioxidant from marine products and potential for industrial production in future.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Berlett, B. S., R. L. Levine and E. R. Stadtman, 1998. Carbon dioxide stimulates peroxynitrite-mediated nitration of tyrosine residues and inhibits oxidation of methionine residues of glutamine synthetase: both modifications mimic effects of adenylylation.Proc. Natl. Acad. Sci. USA 95: 2784–2789.
Bors, W., M. Saran and C. Michel, 1989. Assay of Oxygen Radicals: Method and Mechanisms. Florida, p. 31–62.
Castro, A., M. C. Johnson, M. Anido, A. Cortinez, F. Gabler and M. Vega, 2002. Role of nitric oxide and bcl-2 family genes in the regulation of human endometrial apoptosis.Fertil Steril 3: 587–595.
Frei, B., R. Stocker and B. N. Ames, 1988. Antioxidant defences and lipid peroxidation in human blood plasma.Proc. Natl. Acad. Sci. 85: 9748–9752.
Garlick, P. M., M. J. Davies, D. J. Hearse and T. F. Slater, 1987. Direct detection of free radicals in the reperfused rat heart using electron spin resonance spectroscopy.Circ. Res. 61: 757–760.
Halliwell, B. 1994. Free radicals, antioxidants, and human disease: curiosity, cause, or consequence?Lancet 334: 721–724.
Ho, Y. S., J. L. Magnenat, M. Gargano and J. Cao, 1998. The nature of antioxidant defense mechanisms: a lesson from transgenic studies.Env. Health Perspect S5: 1219–1228.
Kalyanaraman, B., 1996. Detection of nitric oxide by electron spin resonance in chemical, photochemical, cellular, physiological, and pathophysiological systems.Methods Enzymol 268: 168–187.
Prabhu, R. and K. A. Balasubramanian, 2002. Heat preconditioning attenuates oxygen free radical-mediated alterations in the intestinal brush border membrane induced by surgical manipulation.J. Surg. Res. 2: 227–233.
Reed JW, H. H. Ho and W. L. Jolly, 1974. Chemical syntheses with quenched flow reactor hydroxytrihydroborate and peroxynitrite.J. Am. Chem. Soc. 96: 1248–1249.
Rota, C. Y. C. Fann and R. P. Mason, 1999. Phenoxyl free radical formation during the oxidation of the fluorescent dye 2′, 7′-dichlorofluorescein by horseradish peroxidase: possible consequences for oxidative stress measurements.J. Biol. Chem. 274: 28161–28168.
Vasquez-Vivar, J., B. Kalyanaraman and M. C. Kennedy, 2000. Mitochondrial aconitase is a source of hydroxyl radical: an electron spin resonance investigation.J. Biol. Chem. 275: 14064–14069.
Wang, C. B., R. Y. Yao, Z. T. Liu, W. Z. Zhong, X. P. Liu and Y. J. Wang, 2002. Protective effect of polypeptide fromChlamys farreri on hairless mice damaged by ultraviolet A.Acta Pharmacol Sin.9: 813–818.
Wang, P. and J. L. Zweier, 1996. Measurement of nitric oxide and peroxynitrite generation in the postischemic heart: evidence for peroxynitrite-mediated reperfusion injury.J. Biol. Chem. 271: 29223–29230.
Yao, R. Y. and C. B. Wang, 2002. Protective effects of polypeptide from Chlamys farreri on Hela cells damaged by ultraviolet A.Acta Pharmacol. Sin. 11: 1018–1022.
Author information
Authors and Affiliations
Corresponding author
Additional information
Supported by the National Natural Science Foundation of China (No. 39970638) and the Science and Technology Bureau of Qingdao (No. 2001-28-50).
Rights and permissions
About this article
Cite this article
Zhiwu, H., Xiao, C., Chengjuan, L. et al. Free radical scavenging abilities of polypeptide fromChlamys farreri . Chin. J. Ocean. Limnol. 24, 325–328 (2006). https://doi.org/10.1007/PL00020780
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/PL00020780