Skip to main content
SpringerLink
Log in

Stability of a murine hybridoma is dependent on the clonal line and culture media

  • Letter To The Editor
  • Published:
In Vitro Cellular & Developmental Biology - Animal Aims and scope Submit manuscript

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  1. Biedler, J. L.; Delera, P. W.; Spengler, B. A. Chromosome abnormalities and gene amplification: comparison of antifolate-resistant and human neuroblastoma cell systems. In: Rowley, S. D.; Ultmann, J. E., eds. Chromosomes and cancer. NY: Academic Press; 1983:117–138.

    Google Scholar 

  2. Frame, K. K.; Hu, W. S. The loss of antibody productivity in continuous culture of hybridoma cells. Biotech. Bioeng. 35:469–476; 1990.

    Article  CAS  Google Scholar 

  3. Heath, C.; Dilwith, R.; Belfort, G. Methods for increasing monoclonal antibody production in suspension and entrapped cell cultures: biochemical and flow cytometric analysis as a function of medium serum content. J. Biotech. 15:71–89; 1990.

    Article  CAS  Google Scholar 

  4. Kôhler, G.; Milstein, C. Continuous culture of fused cells secreting antibody of predefined specificity. Nature (Lond.) 256:495–497; 1975.

    Article  Google Scholar 

  5. Kontsek, P.; Novak, M.; Kontsekova, E. Karyotype analysis of hybridomas producing monoclonal antibodies against different antigens. Folia Biologica (Praha) 34:99–104; 1988.

    CAS  Google Scholar 

  6. Ozturk, S. S.; Polsson, B. O. Loss of antibody productivity during long-term cultivation of a hybridoma cell line in low serum and serum free media. Hybridoma. 9:167–175; 1990.

    Article  PubMed  CAS  Google Scholar 

  7. Seabright, M. A rapid banding technique for human chromosomes. Lancet. ii:971–972; 1971.

    Article  Google Scholar 

  8. Singleton, J.; Guillen, D.; Gillette, R. W., et al. Stability of antibody production in hybridoma cultures. In Vitro Cell. Dev. Biol. 27:159A; 1991.

    Google Scholar 

  9. Yelton, D. E.; Margulies, D. H.; Diamond, B., et al. Plasmocytomas and hybridomas: development and applications. In: Kennet, R. H.; McKearn, T. J.; Bechtol, K. B., eds. Monoclonal antibodies. Hybridomas, a new dimension in biological analysis. NY and London: Plenum Press; 1980:3–17.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Laboratoire de Virologie, Faculté de Medecine Grange-Blanche, 8 avenue Rockefeller, 69373, Lyon cedex 08, France

    Nicole Kessler & Michèle Aymard

  2. Unité INSERM 218, Centre L. Bérard, 28 rue Laennec, 69373, Lyon cedex 08, France

    Suzanne Bertrand

Authors
  1. Nicole Kessler
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Michèle Aymard
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Suzanne Bertrand
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kessler, N., Aymard, M. & Bertrand, S. Stability of a murine hybridoma is dependent on the clonal line and culture media. In Vitro Cell Dev Biol - Animal 29, 203–207 (1993). https://doi.org/10.1007/BF02634184

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02634184

Keywords

Search

Navigation