Skip to main content
SpringerLink
Log in

Comparison of isopropanol and hexane for extraction of vitamin E and oryzanols from stabilized rice bran

  • Article
  • Published:
Journal of the American Oil Chemists’ Society

Abstract

The effects of solvent-to-bran ratio (2∶1 and 3∶1, w/w), extraction temperature (40 and 60°C), and time (5, 10, 15, 20, and 30 min) were studied for hexane and isopropanol extraction. Increasing the solvent-to-bran ratios and extraction temperature increased the amounts of crude oil, vitamin E and oryzanol recovered for both solvents. An extraction time of 15 min was sufficient for optimum crude oil, vitamin E, and oryzanol extraction. Preheated isopropanol (3∶1 solvent/bran ratio and 60°C) extracted less crude oil (P<.05) but more vitamin E (P<.05) and similar amounts of oryzanol (P>.05) relative to preheated hexane. The data suggest that isopropanol is a promising alternative solvent to hexane for extraction of oil from stabilized rice bran.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Hemavathy, J., and J.V. Prabhakar, Lipid Composition of Rice (Oryza sativa L.) Bran,J. Am. Oil Chem. Soc. 64:1016–1019 (1987).

    CAS  Google Scholar 

  2. Orthoefer, F.T., and R.J. Nicolosi, Rice Bran Oil Composition and Characteristics, presented at the 84th American Oil Chemists' Society Annual Meeting, Anaheim, California, April 25–29, 1993.

  3. Wells, J.H., Utilization of Rice Bran and Oil in Human Diets,Louisiana Agriculture 36:5–8 (1993).

    Google Scholar 

  4. Sharma, R.D., and C. Rukmini, Rice Bran Oil and Hypocholesterolemic in Rats,Lipids 21:715–717 (1986).

    CAS  Google Scholar 

  5. Rukmini, C., Chemical, Nutritional and Toxicological Studies of Rice Bran Oil,Food Chem. 30:257–268 (1988).

    Article  CAS  Google Scholar 

  6. Nicolosi, R.J., L.M. Ausman, and D.M. Hegsted, Rice Bran Oil Lowers Serum Total and Low Density Lipoprotein Cholesterol and Apo B Levels in Nonhuman Primates.Atherosclerosis 88:133–142 (1991).

    Article  CAS  Google Scholar 

  7. Kahlon, T.S., R.M. Saunders, R.N. Sayre, F.I. Chow, M.M. Chiu, and A.A. Betschart, Cholesterol-Lowering Effects of Rice Bran and Rice Bran Oil Fractions in Hypercholesterolemic Hamsters,Cereal Chem. 69:485–489 (1992).

    CAS  Google Scholar 

  8. Hegsted, M., and M.M. Windhauser, Reducing Human Heart Disease Risk with Rice Bran,Louisiana Agriculture 36:22–24 (1993).

    Google Scholar 

  9. Johnson, L.A., and E.W. Lusas, Comparison of Alternative Solvents for Oils Extraction,J. Am. Oil Chem. Soc. 60:229–242 (1983).

    CAS  Google Scholar 

  10. Health and Safety Guide No. 59: n-Hexane Health and Safety Guide, World Health Organization, Geneva, 1991.

  11. Lusas, E.W., L.R., Watkins, and S.S. Koseoglu, Isopropyl Alcohol To Be Tested as Solvent,INFORM 2:970–976 (1991).

    Google Scholar 

  12. Watkins, L.R., S.S. Koseoglu, K.C. Rhee, E. Hernandez, M.N. Riaz, W.H. Johnson, Jr., and S.C. Doty, New Isopropanol System Shows Promise,5:1245–1253 (1994).

    Google Scholar 

  13. Beckel, A.C., P.A. Belter, and A.K. Smith, The Nondistillation Alcohol Extraction Process for Soybean Oil,J. Am. Oil Chem. Soc. 25:10–11 (1948).

    CAS  Google Scholar 

  14. Harris, W.D., F.F. Bishop, C.M. Lyman, and R. Helpert, Isopropanol as a Solvent for Extraction of Cottonseed Oil, I. Preliminary Investigations,24:370–375 (1947).

    CAS  Google Scholar 

  15. Rao, R.K., M.G. Krishna, S.H., Zaheer, and L.K. Arnold, Alcohol Extraction of Vegetable Oils, I. Solubilities of Cottonseed, Peanut, Sesame, and Soybean Oils in Aqueous Ethanol,32:420–423 (1955).

    CAS  Google Scholar 

  16. Rao, R.K., and L.K. Arnold, Alcoholic Extraction of Vegetable Oils. Part I. Solubilities of Vegetable Oils in Aqueous 2-Propanol,34:401–404 (1957).

    CAS  Google Scholar 

  17. Rao, R.K., and L.K. Arnold, Alcoholic Extraction of Vegetable Oils, Pilot Plant Extraction of Cottonseed by Aqueous Ethanol,35:277–281 (1958).

    CAS  Google Scholar 

  18. Baker, E.C., and D.A. Sullivan, Development of a Pilot-Plant Process for the Extraction of Soy Flakes with Aqueous Isopropyl Alcohol,60:1271–277 (1983).

    CAS  Google Scholar 

  19. Hron, R.J., and S.P. Koltun, An Aqueous Ethanol Extraction Process for Cottonseed Oil (Biorenewable Alternative to Hexane),61:1457–1460 (1984).

    CAS  Google Scholar 

  20. Meinke, W.W., B.R. Holland, and W.D. Harris, Solvent Extraction of Rice Bran. Production of B-Vitamin Concentrate and Oil. by Isopropanol Extraction,26:532–534 (1949).

    CAS  Google Scholar 

  21. Talwalkar, R.T., N.K. Grag, and C.R. Krishna Murti, Rice Bran—a Source Material for Pharmaceuticals,J. Food Sci. and Technol. 2:117–119 (1965).

    Google Scholar 

  22. Offical Methods of Analysis of the Association of Official Analytical Chemists, 14th edn., AOAC, Inc., Arlington, 1984.

  23. Shin, T.-S., and J.S. Godber, Improved High-Performance Liquid Chromatography of Vitamin E Vitamers on Normal-Phase Columns,J. Am. Oil Chem. Soc. 70:1289–1991 (1993).

    CAS  Google Scholar 

  24. Shin, T.-S., and J.S. Godber, Isolation of Four Tocopherols and Four Tocotrienols from a Variety of Natural Sources by Semi-Preparative High-Performance Liquid Chromatography,J. Chromatogr. 678:49–58 (1994).

    Article  CAS  Google Scholar 

  25. Kim, I., and Kim, C., Separation of Oryzanol from the Refining By-product of Rice Bran Oil,Korean J. Food Sci. Technol. 23:76–80 (1991).

    Google Scholar 

  26. SAS/STAT User's Guide, Version 6, 4th edn., SAS Institute, Inc., Cary, 1989.

  27. Zhao, W., A. Shishikura, K. Fujimoto, K. Arai, and S. Sait, Fractional Extraction of Rice Bran Oil with Supercritical Carbon Dioxide,Agric. and Biol. Chem. 51:1773–1777 (1987).

    CAS  Google Scholar 

  28. Nicolosi, R.J., E.J. Rogers, L.M. Austman, and F.T. Orthoefer, Rice Bran Oil and Its Health Benefits, inRice Science and Technology, edited by W.E. Marshall and J.I. Wadsworth, Marcel Dekker, Inc., New York, 1994, pp. 421–437.

    Google Scholar 

  29. Kim, C.J.,-S.M. Byun, H.S. Cheigh, and T.W. Kwon, Comparison of Solvent Extraction Characteristics of Rice Bran Pretreated by Hot Air Drying, Steam Cooking and Extrusion,J. Am. Oil Chem. Soc. 64:514–516 (1987).

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biological and Agricultural Engineering, Louisiana State University Agricultural Center, 70803, Baton Rouge, Louisiana

    Weicheng Hu & John Henry Wells

  2. Department of Food Science, Louisiana Agricultural Experiment Station, Louisiana State University Agricultural Center, 70803, Baton Rouge, Louisiana

    Tai-Sun Shin & J. Samuel Godber

Authors
  1. Weicheng Hu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. John Henry Wells
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Tai-Sun Shin
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. J. Samuel Godber
    View author publications

    You can also search for this author in PubMed Google Scholar

About this article

Cite this article

Hu, W., Wells, J.H., Shin, TS. et al. Comparison of isopropanol and hexane for extraction of vitamin E and oryzanols from stabilized rice bran. J Am Oil Chem Soc 73, 1653–1656 (1996). https://doi.org/10.1007/BF02517967

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02517967

Key Words

Search

Navigation