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Particle recognition by haemocytes from the colonial ascidianBotrylloides leachii: Evidence that theB. leachii HA-2 agglutinin is opsonic

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Summary

Haemocytes from the ascidianBotrylloides leachii were observed in vivo to phagocytose sheep erythrocytes. The possibility that a sheep erythrocyte agglutinin (the HA-2 agglutinin) previously purified fromB. leachii haemolymph functions as a recognition molecule for the phagocytosis of these erythrocytes was investigated.

Untreated sheep erythrocytes were found to adhere toB. leachii haemocytes in vitro. Adherence appeared to be mediated by the HA-2 agglutinin, as evidenced by the inhibition of adhesion by lactose (which is a specific inhibitor of the HA-2 agglutinin) and by an anti-HA-2 IgG preparation. Immunofluorescence studies indicated that HA-2 molecules secreted by the haemocytes bound to unsensitised erythrocytes, causing them to adhere to haemocytes. No HA-2 agglutinin could be detected on the surface of the haemocytes in the absence of erythrocytes but receptors for the agglutinin were detected. The results suggest that the HA-2 agglutinin can function as a recognition molecule for sheep erythrocytes and other particles bearing the appropriate carbohydrate moieties on their surfaces. At least one of two other lectins purified from haemolymph (HA-1 and LBP-3) was detected by immunofluorescence on the surface of haemocytes. The function(2) of these latter molecules, neither of which binds to sheep erythrocytes, is not known.

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Abbreviations

BSS :

Botrylloides salt solution

HA-1 :

haemagglutinin one

HA-2 :

haemagglutinin two

IgG :

immunoglobulin G

LBP-3 :

lactose binding protein three

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Authors and Affiliations

  1. Department of Zoology and Department of Microbiology and Immunology, The University of Adelaide, Box 498, G.P.O., 5001, Adelaide, South Australia

    D. R. Coombe, P. L. Ey & C. R. Jenkin

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  1. D. R. Coombe
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  2. P. L. Ey
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  3. C. R. Jenkin
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Coombe, D.R., Ey, P.L. & Jenkin, C.R. Particle recognition by haemocytes from the colonial ascidianBotrylloides leachii: Evidence that theB. leachii HA-2 agglutinin is opsonic. J Comp Physiol B 154, 509–521 (1984). https://doi.org/10.1007/BF02515156

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