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Measurement of amyloglucosidase usingP-nitrophenyl β-maltoside as substrate

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Biotechnology Techniques

Summary

An enzyme-linked assay for the measurement of amyloglucosidase in commercial enzyme mixtures and crude culture filtrates is described. A method for the synthesis of the substrate employed,p-nitrophenyl β-D-maltoside, is also described. The substrate is used in the presence of saturating levels of β-glucosidase. With a range ofAspergillus sp. culture filtrates, an excellent correlation was found for values obtained with this assay and a conventional assay employing maltose as substrate with measurement of released glucose.

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Author information

Authors and Affiliations

  1. Megazyme (Austr.) Pty. Ltd., 6 Altona Place, 2151, North Rocks, N.S.W., Australia

    Barry V. McCleary

  2. Centre de Recherches sur les Macromolecules Vegetales (CERMAV-CNRS), BP 53, X 38041, Grenoble cedex, France

    Francoise Bouhet & Hugues Driguez

Authors
  1. Barry V. McCleary
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  2. Francoise Bouhet
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  3. Hugues Driguez
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McCleary, B.V., Bouhet, F. & Driguez, H. Measurement of amyloglucosidase usingP-nitrophenyl β-maltoside as substrate. Biotechnol Tech 5, 255–258 (1991). https://doi.org/10.1007/BF02438658

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  • DOI: https://doi.org/10.1007/BF02438658

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