Summary
Incorporation of Virazole into potato explant and meristem culture media results in a higher percentage of virus-free progeny plants from virus-infected explant and meristem donor plants than are produced in tissue cultures in the absence of Virazole. Viruses eliminated, singly and as complexes, include potato viruses X, Y, S and M.
The need for sensitive virus tests to distinguish between virus suppression and elimination is discussed and protein-A-linked immunoelectron microscopy is described and quantified for potato virus X.
Zusammenfassung
In der vorliegenden und einer früheren Arbeit (Cassells & Long, 1980) zeigte Kultivierung von Explantaten auf einem Medium für Gewebekultur mit Virazole (Ribavirin) einen signifikanten Anstieg virusfreier Adventivschosse gegenüber der Kontrolle. Diese Adventivschosse wie auch Kartoffelpflanzen zweiter Generation verhielten sich auch gegenüber den empfindlichsten immunoelektronen-mikroskopischen Tests virusfrei.
In Gegenwart von Virazol ergab auch Meristemkultur einen höheren Anteil virusfreier Pflanzen als bei Fehlen dieser Verbindung.
Die Kartoffelviren X, Y, M und S sowie Viruskomplexe konnten aus den Sorten May Queen, King Edward, Kerr's Pink und Golden Wonder eliminiert werden (Tabellen 2 und 3).
Die Ergebnisse der Anwendung und der Quantifizierung der Protein A-gebundenen Immuno-Elektronenmikroskopie (PALIEM) werden ebenfalls dargestellt (Abb. 1).
Résumé
Cette étude a montré, comme d'autres travaux préliminaires (Cassels & Long, 1980), que le fait de cultiver des boutures sur un milieu de culture de tissus contenant du virazole (ribavirine) permettait d'augmenter de façon significative le nombre de tiges aventices indemmes de virus par rapport au témoin. Ces tiges adventices, dans le cas de plants de deuxième génération, sont apparues indemmes de virus par l'analyse des tests les plus sensibles de microscopie immuno-électronique.
En présence du virazole, la culture de méristèmes montre également un taux plus élevé de plants indemmes de virus que sans l'application de virazole.
Les virus concernés sont les virus X, Y, M, S ainsi, que les complexes viraux, provenant des variétés May Queen, King Edward, Kerr's Pink et Golden Wonder (tableau 2 et 3).
Sont également indiqués les résultats qualificatifs et quantitatifs de la microcopie immunoélectronique avec utilisation de la protéine A (PALIEM) (figure 1).
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References
Bailiss, K. W., F. M. Cocker & A. C. Cassells, 1977. The effect of benlate and cytokinins on the content of tobacco mosaic virus in tomato leaf disks and cucumber mosaic virus in cucumber cotyledon disks and seedlings.Ann. appl. Biol. 87: 383–392.
Cassells, A. C., 1979. The effect of 2,3,5-triiodobenzoic acid on caulogenesis in callus cultures of tomato and pelargonium.Physiologia Plant. 46: 59–165.
Cassells, A. C. & R. D. Long, 1980. The regeneration of virus-free plants from cucumber mosaic virus- and potato virus Y-infected tobacco explants cultures in the presence of virazole.Z. Naturforsch. 35c: 350–351.
Cassells, A. C., G. Minas & R. Long, 1980. Comparative Tissue Culture studies ofPelargonium hybrids using meristem and explant culture: Chimeral and beneficially-infected varieties. In: D. S. Ingram & J. P. Helgeson (Ed.). Tissue culture for plant propagators. Blackwell Scientific Publications, Oxford, pp. 93–96.
Clark, M. F. & A. N. Adams, 1977. Characteristics of micro plate method of enzyme-linked immunosorbent assay for the detection of plant viruses.J. gen. Virol. 34: 475–483.
Fazio, G. de, J. Caner & Marly Vicente, 1978. Inhibitory effect of virazole (ribavirin) on the replication of tomato white necrosis virus (VNBT).Arch. Virol. 58: 153–156.
Fazio, G. de, J. Caner & Marly Vicente, 1980. Effect of Virazole (ribavirin) on tomato spotted wilt virus in two systemic hosts, tomato and tobacco.Arch. Virol. 63: 305–309.
Gough, K. H. & D. D. Shukla, 1980. Further studies on the use of Protein A in immune electron microscopy for detecting virus particles.J. gen. Virol. 51: 415–419.
Horvath, J., 1967. Separation and determination of viruses pathogenic to potatoes with special regard to potato virus Y.Acta phytopath. 2: 319–359.
Kluge, S. & K. Marcinka, 1979. The effects of polyacrylic acid and virazole on the replication and component formation of red clover mottle virus.Acta virol. 23: 148–152.
Lerch, B., 1977. Inhibition of the biosynthesis of potato virus X by ribavirin.Phytopath. Z. 89: 44–49.
Migus, D. O. & P. Dobos, 1980. Effect of infectious pancreatic necrosis virus in fish cell cultures.J. gen. Virol. 47: 47–57.
Miller, J. P., L. J. Kigwana, D. G. Streeter, R. K. Robbins, L. N. Simon & J. Roboz, 1976. The relationship between the metabolism of ribavirin and its proposed mechanism of action.Ann. N. Y. Acad. Sci. 284: 211–229.
Noordam, D., 1973. Identification of plant viruses. Pudoc, Wageningen, p. 41.
Quak, F., 1972. In: J. A. de Bokx (Ed.), Viruses of potatoes and seed potato production. Pudoc, Wageningen, pp. 158–166.
Sampson, P. J. & R. H. Taylor, 1968. A comparison of electron microscopy, microprecipitin and indicator plants for the detection of potato viruses S, X and Y.Phytopathology 58: 489–493.
Schuster, G., 1979. On some interactions of 1-β-D-ribofuranosyl-1,2,4-triazole-3-carboxamide (Virazole) and plant hormones in virusinfected plants.Phytopath. Z. 94: 72–79.
Sidwell, O. W., L. N. Simon, J. W. Witkowski & R. K. Robbins, 1974. Antiviral activity of virazole. Review and structure-activity relationships.Progr. Chemotherapy 2: 889–903.
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Cassells, A.C., Long, R.D. The elimination of potato viruses X, Y, S and M in meristem and explant cultures of potato in the presence of Virazole. Potato Res 25, 165–173 (1982). https://doi.org/10.1007/BF02359803
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DOI: https://doi.org/10.1007/BF02359803