Synopsis
The ability of embryos of the viviparous scorpaenidSebastes melanops to take up nutrients from an exogenous substrate was demonstrated by incubating embryos at various stages of development (18–30 days after fertilization) in14C-labeled glycine for 24 h. Uptake was highest for embryos at the latest stages (28–30 days) and increased at a linear rate during the incubation period. Nutrient uptake was not time dependent in embryos at the early stages (18–22 days). Nutrient utilization byS. melanops embryos was measured by the oxidation of14C-labeled glycine to14CO2. The amount of respired14CO2 by the oldest embryos increased significantly at a linear rate over the 24 h incubation period. There was no evidence of nutrient utilization by the youngest embryos. The developmental changes we observed in the uptake and utilization of exogenous glycine are supported by our previous findings that the oldest embryos have fully developed mouths and guts, and require additional nutrition from intraovarian sources at this stage of development.
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References cited
Balon, E.K. 1981. Additions and amendments to the classification of reproductive styles in fishes. Env. Biol. Fish. 6: 377–389.
Boehlert, G.W., M. Kusakari, M. Shimizu & J. Yamada. 1986. Energetics during embryonic development in kurosoi,Sebastes schlegeli Hilgendorf. J. Exp. Mar. Biol. Ecol. 101: 239–256.
Boehlert, G.W. & M.M. Yoklavich. 1984. Reproduction, embryonic energetics, and the maternal-fetal relationship in the viviparous genusSebastes (Pisces: Scorpaenidae). Biol. Bull. 167: 354–370.
Cornish, D.A. & W.J. Veith. 1986. Embryonic adaptations and nutrition in the viviparous teleostClinus dorsalis (Perciformes: Clinidae). S. Afr. Tydskr. Dierk. 21: 79–84.
Dobbs, G.H. 1975. Scanning electron microscopy of intraovarian embryos of the viviparous teleostMicrometrus minimus (Gibbons), (Perciformes: Embiotocidae). J. Fish Biol. 7: 209–214.
Dygert, P.H. & D.R. Gunderson. 1991. Energy utilization by embryos during gestation in viviparous copper rockfish,Sebastes caurinus. Env. Biol. Fish. 30: 165–171.
Forster, R.P. & S.K. Hong. 1958.In vitro transport of dyes by isolated renal tubules of the fluonder as disclosed by direct visualization: intracellular accumulation and transcellular movement. J. Cell. Comp. Physiol. 51: 259–272.
Gahan, P.B. (ed.) 1972. Autoradiography for biologists. Academic Press, London. 124 pp.
Grove, B.D. & J.P. Wourms. 1982. Embryonic nutrient absorption in the poeciliidHeterandria formosa. Amer. Zool. 22: 881.
Hart, J.L. 1973. Pacific fishes of Canada. Fish. Res. Board Can. Bull. 180: 1–740.
Laroche, W.A. & S.L. Richardson. 1980. Development and occurrence of larvae and juveniles of the rockfishesSebastes flavidus andSebastes melanops (Scorpaenidae) off Oregon. U.S. Fish. Bull. 77: 901–924.
Lombardi, J. & J.P. Wourms. 1985. The trophotaenial placenta of a viviparous goodeid fish. III: Protein uptake by trophotaeniae, the embryonic component. J. Exp. Zool. 236: 165–179.
O'Connell, C.P. 1976. Histological criteria for diagnosing the starving condition in early post yolk sac larvae of the northern anchovy,Engraulis mordax Girard. J. Exp. Mar. Biol. Ecol. 25: 285–312.
Shimizu, M., M. Kusakari, M.M. Yoklavich, G.W. Boehlert & J. Yamada. 1991. Ultrastructure of the epidermis and digestive tract inSebastes embryos, with special reference to the uptake of exogenous nutrients. Env. Biol. Fish. 30: 155–163.
Shimizu, M. & J. Yamada. 1980. Ultrastructural aspects of yolk absorption in the vitelline syncytium of the embryonic rockfish.Sebastes schlegeli. Jap. J. Ichthyol. 27: 56–63.
Stahl-Johnson, K.L. 1984. Rearing and development of larvalSebastes caurinus (copper rockfish) andS. auriculatus (brown rockfish) from the northeastern Pacific. M.Sc. Thesis, University of Washington, Seattle. 218 pp.
Theilacker, G.H. 1978. Effect of starvation on the histological and morphological characteristics of jack mackerel,Trachurus symmetricus, Larvae. U.S. Fish. Bull. 76: 403–414.
Turner, C.L. 1937. The trophotaeniae of the Goodeidae, a family of viviparous cyprinodont fishes. J. Morphol. 61: 495–523.
Veith, W.J. 1979. The chemical composition of the follicular fluid of the viviparous teleostClinus superciliosus. Comp. Biochem. Physiol. 63A: 37–40.
Veith, W.J. 1980. Viviparity and embryonic adaptations in the teleostClinus superciliosus. Can. J. Zool. 58: 1–12.
Webb, P.W. & J.R. Brett. 1972. Respiratory adaptations of prenatal young in the ovary of two species of viviparous seaperch,Rhacochilus vacca andEmbiotoca lateralis. J. Fish. Res. Board Can. 29: 1525–1542.
Wourms, J.P. 1981. Viviparity: the maternal-fetal relationship in fishes. Amer. Zool. 21: 473–515.
Wourms, J.P., B.D. Grove & J. Lombardi. 1988. The maternalembryonic relationship in viviparous fishes. pp. 1–135.In: W.S. Hoar & D.J. Randall (ed.) Fish Physiology, vol. 11 (part B). Academic Press, San Diego.
Yamada, J. & M. Kusakari. 1991. Staging and the time course of embryonic development in kurosoi,Sebastes schlegeli. Env. Biol. Fish. 30: 103–110.
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Yoklavich, M.M., Boehlert, G.W. Uptake and utilization of14C-glycine by embryos ofSebastes melanops . Environ Biol Fish 30, 147–153 (1991). https://doi.org/10.1007/BF02296885
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DOI: https://doi.org/10.1007/BF02296885