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Bacterial adhesion: A physicochemical approach

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Abstract

The adhesion of bacteria to solid surfaces was studied using a physicochemical approach. Adhesion to negatively charged polystyrene was found to be reversible and could be described quantitatively using the DLVO theory for colloidal stability, i.e., in terms of Van der Waals and electrostatic interactions. The influence of the latter was assessed by varying the electrolyte strength. Adhesion increased with increasing electrolyte strength. The adhesion Gibbs energy for a bacterium and a negatively charged polystyrene surface was estimated from adhesion isotherms and was found to be 2–3 kT per cell. This low value corresponds to an adhesion in the secondary minimum of interaction as described by the DLVO theory. The consequences of these findings for adhesion in the natural environment are discussed.

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References

  1. Absolom DR, Lamberti FV, Policova Z, Zingg W, van Oss CJ, Neumann AW (1983) Surface thermodynamics of bacterial adhesion. Appl Environ Microbiol 46:90–97

    PubMed  Google Scholar 

  2. Alieva RM, Manasbaeva AB, Ilyaletdinov AN (1987) Immobilization of microorganisms on a latex in order to obtain an artificial floc. Mikrobiol 55:692–699

    Google Scholar 

  3. Baker JH (1984) Factors affecting the bacterial colonization of various surfaces in a river. Can J Microbiol 30:511–515

    Google Scholar 

  4. Beeftink HH, Staugaard P (1986) Structure and dynamics of anaerobic bacterial aggregates in a gas-lift reactor. Appl Environ Microbiol 52:1139–1146

    Google Scholar 

  5. Busscher HJ, Weerkamp AH, van der Mei HL, van Pelt AWJ, de Jong HP, Arends J (1984) Measurement of the surface-free energy of bacterial cell surfaces and its relevance for adhesion. Appl Environ Microbiol 48:980–983

    PubMed  Google Scholar 

  6. Busscher HJ, Uyen MHMJC, Weerkamp AH, Postma WJ, Arends J (1986) Reversibility of adhesion of oral streptococci to solids. FEMS Microbiol Lett 35:303–306

    Article  Google Scholar 

  7. Costerton JW (1974) Structrure and function of the cell envelope of gram-negative bacteria. Bacteriol Rev 38:87–110

    PubMed  Google Scholar 

  8. Dazzo FB (1984) Bacterial adhesion to plant root surfaces. In: KC Marshall (ed) Microbial adhesion and aggregation. Springer Verlag, Berlin, pp 85–93

    Google Scholar 

  9. Fletcher M (1977) The effect of culture concentration and age, time and temperature on bacterial attachment to polystyrene. Can J Microbiol 23:1–6

    Google Scholar 

  10. Fletcher M, Loeb GI (1979) Influence of substratum characteristics on the attachment of a marine pseudomonad to solid surfaces. Appl Environ Microbiol 37:1–67

    PubMed  Google Scholar 

  11. Gafny R, Okon Y, Kapulnik Y, Fisher M (1986) Adsorption ofAzospirillum brasilense to corn roots. Soil Biol Biochem 18:69–75

    Article  Google Scholar 

  12. Gordon AS, Millero FJ (1984) Electrolyte effects on attachment of an estuarine bacterium. Appl Environ Microbiol 47:495–499

    Google Scholar 

  13. Haecht JL van, Bolipombo M, Rouxhet PG (1984) Immobilization ofSaccharomyces cerevisiae by adhesion: Treatment of the cells by Al ions. Biotechnol Bioeng 27:217–224

    Article  Google Scholar 

  14. Hattori R, Hattori T (1985) Adsorptive phenomena involving bacterial cells and an anion exchange resin. J Gen Appl Microbiol 31:147–163

    Google Scholar 

  15. Hoght AH, Dankert J, de Vries JA, Feyen J (1983) Adhesion of coagulase negative staphylococci to biomaterials. J Gen Microbiol 129:2959–2968

    PubMed  Google Scholar 

  16. Jones MN (1975) Biological interfaces: An introduction to the surface and colloid science of biochemical and biological systems. Elseviers, Amsterdam

    Google Scholar 

  17. Kluepfel DA (1986) Adsorption ofAgrobacterium tumefaciens to susceptible potato tissues: A physisorption process. Appl Environ Microbiol 51:1130–1132

    Google Scholar 

  18. Kruyt HR, Klompé MAM (1942) Solkonzentration und Flockung beim AgJ-sol. Kolloid-Beihefte 54:484–553

    Google Scholar 

  19. Kuhn E, Loosdrecht M van, Giger W, Schwarzenbach RP (1987) Microbial degradation of nitrilitriacetate (NTA) during riverwater/groundwater infiltration: Laboratory column studies. Water Res 21:1237–1248

    Article  Google Scholar 

  20. Larsson K, Glantz PO (1981) Microbial adhesion to surfaces with different surface charges. Acta Odontol Scand 39:79–82

    PubMed  Google Scholar 

  21. Loder TC, Liss PS (1985) Control by organic coatings of the surface charge of estuarine suspended particles. Limnol Oceanogr 30:418–421

    Google Scholar 

  22. Loosdrecht MCM van, Lyklema J, Norde W, Schraa G, Zehnder AJB (1987) The role of bacterial cell wall hydrophobicity in adhesion. Appl Environ Microbiol 53:1893–1897

    PubMed  Google Scholar 

  23. Loosdrecht MCM van, Lyklema J, Norde W, Schraa G, Zehnder AJB (1987) Electrokinetic potential and hydrophobicity as a measure to predict the initial steps of bacterial adhesion. Appl Environ Microbiol 53:1898–1901

    PubMed  Google Scholar 

  24. Marshall KC, Stout R, Mitchell R (1971) Mechanisms in the initial events in the sorption of marine bacteria to surfaces. J Gen Microbiol 68:337–348

    Google Scholar 

  25. Matthyse AG, Holmes KV, Gurlitz RWG (1981) Elaboration of cellulose fibrils byAgrobacterium tumefaciens during attachment to carrot cells. J Bacteriol 145:583–595

    PubMed  Google Scholar 

  26. Nikovskaya GN, Gordienka AS, Globa LI (1987) Sorption of microorganisms by fibrious materials. Mikrobiol 55:545–548

    Google Scholar 

  27. Nir S (1976) Van der Waals interactions between surfaces of biological interest. Progr Surf Sci 8:1–58

    Article  Google Scholar 

  28. Nordin JS, Tsuchiya HM, Fredrickson AG (1967) Interfacial phenomena governing adhesion ofChlorella to glass surfaces. Biotechnol Bioeng 9:545–558

    Article  Google Scholar 

  29. Pelt AWJ van, Weerkamp AH, Uyen MHWJC, Busscher HJ, de Jong HP, Arends J (1980) Adhesion ofStreptococcus sanguis CH-3 to polymers with different surface-free energies. Appl Environ Microbiol 49:1270–1275

    Google Scholar 

  30. Powell MS, Slater NKH (1982) Removal rates of bacterial cells from glass surfaces by fluid shear. Biotechnol Bioeng 24:2527–2537

    Article  Google Scholar 

  31. Puepke SG, Benny UK (1984) Adsorption of tumorgenicAgrobacterium tumefaciens cells to susceptible potato tuber tissues. Can J Microbiol 30:1030–1037

    PubMed  Google Scholar 

  32. Rosenberg M, Kjelleberg S (1986) Hydrophobic interactions: Role in bacterial adhesion. Adv Microbial Ecol 9:353–393

    Google Scholar 

  33. Rouxhet PG, Mozes N, Haecht JL van, Reuliause L, Palm-Gennen MH (1984) Immobilization of microbial cells by adhesion to a support. In: Proc 3rd European Congress on Biotechnology. Verlag Chemie, Weinheim, pp I 319–325

    Google Scholar 

  34. Rutter PR, Vincent B (1984) Physicochemical interactions of the substratum, microorganisms, and the fluid phase. In: Marshall KC (ed) Microbial adhesion and aggregation. Springer Verlag, Berlin, pp 21–38

    Google Scholar 

  35. Schlegel HG (1974) Allgemeine Mikrobiologie. Georg Thieme Verlag, Stuttgart

    Google Scholar 

  36. Smit G, Kijne JW, Lugtenberg BJJ (1987) Involvement of both cellulose fibrils and a Ca2+-dependent adhesion in the attachment ofRhizobium leguminosarum to pea root hair tips. J Bacteriol 169:4294–4301

    PubMed  Google Scholar 

  37. Stotzky G (1985) Mechanisms of adhesion to clays, with reference to soil systems. In: Savage DC, Fletcher M (eds) Bacterial adhesion. Plenum Press, New York, pp 195–253

    Google Scholar 

  38. Turakhia MH, Cooksey KE, Characklis WG (1983) Influence of a calcium-specific chelant on biofilm removal. Appl Environ Microb 46:1236–1238

    Google Scholar 

  39. Volmer M (1925) Thermodynamische Folgerungen aus der Zustandsgleichung für adsorbierte Stoffe. Z Phys Chem 115:253–260

    Google Scholar 

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Author notes

  1. Mark C. M. van Loosdrecht

    Present address: Department of Bioprocesstechnology, Technical University, Delft, The Netherlands

Authors and Affiliations

  1. Department of Microbiology, Agricultural University, Hesselink van Suchtelenweg 4, 6703, CT Wageningen, The Netherlands

    Mark C. M. van Loosdrecht & Alexander J. B. Zehnder

  2. Department of Physical and Colloid Chemistry, Agricultural University, De Dreijen 6, 6703, BC Wageningen, The Netherlands

    Johannes Lyklema & Willem Norde

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  1. Mark C. M. van Loosdrecht
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  2. Johannes Lyklema
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  3. Willem Norde
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  4. Alexander J. B. Zehnder
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van Loosdrecht, M.C.M., Lyklema, J., Norde, W. et al. Bacterial adhesion: A physicochemical approach. Microb Ecol 17, 1–15 (1989). https://doi.org/10.1007/BF02025589

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