Abstract
Anaphylaxis in the isolated guinea-pig heart was associated with a sudden release of histamine with a long-lasting release of nitrite (NO −2 ), an oxidation product of NO.N G-monomethyl-l-arginine (MeArg, 300 μM) increased the severity of cardiac anaphylaxis, as shown by the decrease in the coronary flow and by a prolonged duration of antigen-induced arrhythmias. Concomitantly, MeArg increased the release of histamine while decreasing the release of nitrite. Sodium nitroprusside (NaNP, 10−5–10−4 M) reduced the severity of cardiac anaphylaxis by increasing coronary flow and shortening the duration of antigen-induced arrhythmias. Concomitantly, NaNP decreased the release of histamine while increasing the release of nitrite. In mast cells isolated from actively sensitized guinea-pigs, the release of histamine elicited by specific antigen was increased by MeArg and decreased by NaNP.
In conclusion, endogenous and exogenous NO antagonizes the effect of vasoconstrictor mediators released after antigen challenge and plays a protective role in anaphylactic reactions “in vitro”,
Similar content being viewed by others
References
D. Salvemini, E. Masini, A. Pistelli, P. F. Mannaioni and J. Vane,Nitric oxide: A regulatory mediator of mast cell reactivity. J. Cardiovasc. Pharmacol.17, S258-S264 (1991).
K. I. Thelen, A. Dembinska-Kiéc, D Pallapies, Th. Simmet and B. A. Peskar,Effect of 3-morpholinosydnonimine (SIN-1) and N ω-nitro-l-arginine (NNA)on isolated perfused anaphylactic guinea-pig hearts. Naunyn-Schiedeberg's Arch. Pharmacol.345, 93–99 (1992).
P. Blandina, S. Brunelleschi, R. Fantozzi, E. Gianella, P. F. Mannaioni and E. Masini,The anaphylactic action of histamine H 2-receptor agonists in the guinea-pig isolated heart. Br. J. Pharmacol.90, 459–466 (1987).
L. C. Green, D. Wagner, A. Glogowski, J. Skipper, J. S. Wishnok and S. R. Tannenbaum,Analysis of nitrate, nitrite and N-nitrate in biological fluids. Anal. Biochem.126, 131–138 (1982).
F. L. Pearce and M. Ennis,Isolation and some properties of mast cells from the mesentery of the rat and guinea pig. Agents Actions10, 124–131 (1980).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Masini, E., Gambassi, F., Di Bello, M.G. et al. Nitric oxide modulates cardiac and mast cell anaphylaxis. Agents and Actions 41 (Suppl 1), C89–C90 (1994). https://doi.org/10.1007/BF02007780
Issue Date:
DOI: https://doi.org/10.1007/BF02007780