Summary
A method for the isolation of nucleate and anucleate fragments ofSphaerechinus granularis unfertilized eggs is described. It is based on centrifugation in a sucrose gradient; its yield is sufficient to allow enzymatic studies to be made.
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References
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Aimi, J. Isolement de fragments nucléés et anucléés d'œufs de l'oursinSphaerechinus granularis. Experientia 30, 837–838 (1974). https://doi.org/10.1007/BF01924221
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DOI: https://doi.org/10.1007/BF01924221