European Journal of Plant Pathology

, Volume 102, Issue 3, pp 305–310

A carlavirus-specific PCR primer and partial nucleotide sequence provides further evidence for the recognition of cowpea mild mottle virus as a whitefly-transmitted carlavirus

  • J. Badge
  • A. Brunt
  • R. Carson
  • E. Dagless
  • M. Karamagioli
  • S. Phillips
  • S. Seal
  • R. Turner
  • G. D. Foster
Short Communication

DOI: 10.1007/BF01877970

Cite this article as:
Badge, J., Brunt, A., Carson, R. et al. Eur J Plant Pathol (1996) 102: 305. doi:10.1007/BF01877970
  • 257 Views

Abstract

Cowpea mild mottle virus (CMMV) has physicochemical properties typical of carlaviruses, but has remained unclassified due to a number of unusual properties, including no serological cross-reaction with 18 carlaviruses; production of brush-like inclusion bodiesin vivo; and the ability to be transmitted by whiteflies (Bermisia tabaci). In this paper we report the use of a carlavirus specific PCR primer to identify CMMV as a member of the carlavirus group. This is confirmed by nucleotide sequence (958 nucleotides) from the 3′ terminal region of CMMV RNA which contains a partial open reading frame (ORF) having high similarity with the coat proteins of other carlaviruses. The sequence also contains an 11.7K ORF at the 3′ terminus, containing a ‘zinc-finger’ motif which is unique to carlaviruses.

Key words

carlavirus-specific primerCMMVnucleotide sequencePCR

Copyright information

© Kluwer Academic Publishers 1996

Authors and Affiliations

  • J. Badge
    • 1
  • A. Brunt
    • 2
  • R. Carson
    • 1
  • E. Dagless
    • 1
  • M. Karamagioli
    • 3
  • S. Phillips
    • 2
  • S. Seal
    • 3
  • R. Turner
    • 1
  • G. D. Foster
    • 1
  1. 1.Botany DepartmentUniversity of LeicesterLeicesterUK
  2. 2.HRILittlehamptonUK
  3. 3.NRIChatham MaritimeUK