Skip to main content
SpringerLink
Log in

Attachment and entry of infectious pancreatic necrosis virus (IPNV) into CHSE-214 cells

  • Brief Report
  • Published:
Archives of Virology Aims and scope Submit manuscript

Summary

Infectious pancreatic necrosis virus (IPNV) attaches to CHSE-214 cells through two types of cell components: specific and non-specific ones. Competition experiments with inactivated IPNV showed that IPNV requires specific components to productively infect cells. Just a low amount of adsorbed IPNV enters the cell. After 20 minutes, part of the adsorbed IPNV was internalized into acid compartments. Also, the viruses adsorbed on the cell surface require similar periods of time to escape from the neutralization of antibodies.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  1. Brown F (1986) The classification and nomenclature of viruses: summary of results of meetings of the International Committee on Taxonomy of Viruses in Sendai, September, 1984. Intervirology 25: 140–143

    Google Scholar 

  2. Carlsson A, Kuznar J, Varga M, Everitt E (1994) Purification of infectious pancreatic necrosis virus by anion exchange chromatography increases the specific activity. J Virol Methods 47: 27–35

    PubMed  Google Scholar 

  3. Couve E, Kiss J, Kuznar J (1992) Infectious pancreatic necrosis virus internalization and endocytic organelles in CHSE-214 cells. Cell Biol Int Rep 16: 899–906

    PubMed  Google Scholar 

  4. Dimmock NJ (1982) Initial stages in infection with animal viruses. J Gen Virol 59: 1–22

    PubMed  Google Scholar 

  5. Dobos P, Hallet R, Kells DTC, Sorensen O, Rowe D (1977) Biophysical studies of infectious pancreatic necrosis virus. J Virol 22: 150–159

    PubMed  Google Scholar 

  6. Dorson M (1982) Infectious pancreatic necrosis virus of salmonids. In: Anderson DP, Dorson M, Dubourget PH (eds) Antigens of fish pathogens; development and production for vaccines and serodiagnostics. Collection Fondation Marcel Merieux, Lyon, pp 1–32

    Google Scholar 

  7. Espinoza E, Farías G, Soler M, Kuznar J (1985) Identity between infectious pancreatic necrosis virus VR-299 and a Chilean isolate. Intervirology 24: 58–60

    PubMed  Google Scholar 

  8. Estay A, Farías G, Soler M, Kuznar J (1990) Further analysis on the structural proteins of infectious pancreatic necrosis virus. Virus Res 15: 85–96

    PubMed  Google Scholar 

  9. Farías G, Navarrete E, Kiss, J, Kuznar J (1988) Effect of ammonium chloride on the multiplication of infectious pancreatic necrosis virus. Arch Virol 98: 155–162

    PubMed  Google Scholar 

  10. Lenard J (1986) Uncoating of vesicular estomatitis virus. In: Crowell RL, Lonberg-Holm K (eds) Virus attachment and entry into cells. American Society for Microbiology, pp 182–190

  11. Marsh M, Helenius A (1989) Virus entry into animal cells. Adv Virus Res 36: 107–151

    PubMed  Google Scholar 

  12. Somogyi P, Dobos P (1980) Virus-specific RNA synthesis in cells infected by infectious pancreatic necrosis virus. J Virol 33: 129–139

    Google Scholar 

  13. Yoshimura A, Ohnishi S (1984) Uncoating of influenza virus in endosomes. J Virol 51: 497–504

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Laboratorio de Bioquimica, Instituto de Ciencias Biológicas y Quimicas, Facultad de Ciencias, Universidad de Valparaiso, Valparaíso, Chile

    J. Kuznar, M. Soler, G. Farias & J. C. Espinoza

Authors
  1. J. Kuznar
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. Soler
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. G. Farias
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. J. C. Espinoza
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kuznar, J., Soler, M., Farias, G. et al. Attachment and entry of infectious pancreatic necrosis virus (IPNV) into CHSE-214 cells. Archives of Virology 140, 1833–1840 (1995). https://doi.org/10.1007/BF01384345

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01384345

Keywords

Search

Navigation