Skip to main content
SpringerLink
Log in

High similarity among the tomato yellow leaf curl virus isolates from the West Mediterranean Basin: the nucleotide sequence of an infectious clone from Spain

  • Brief Report
  • Published:
Archives of Virology Aims and scope Submit manuscript

Summary

An isolate of tomato yellow leaf curl geminivirus, from the first epidemic outbreaks that occurred in Murcia, Spain (TYLCV-M) in 1992, was cloned and its nucleotide sequence was determined. The circular single stranded DNA consisted of 2777 nucleotides. The genome organization resembled that of other TYLCV sequenced so far; regulatory signal sequences for bidirectional transcription and for polyadenylation of the transcripts were localized in the sequence. Infectivity of the cloned DNA was demonstrated by subcloning a 1.8 mer of TYLCV-M in pBin19 and agroinoculating it into tomato andNicotiana benthamiana plants. Symptoms and viral DNA forms in agroinfected plants did not differ from those of field infected ones. Sequence comparisons with other TYLCV isolates show a high homogeneity between isolates from the West Mediterranean Basin, suggesting the presence of a geographical cluster.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  1. Abdallah NA, Aref NM, Fauquet CM, Madkour MA, Beachy RH (1993) Nucleotide sequence and genome organization of an infectious DNA clone of tomato yellow leaf curl virus isolated from Egypt. Proceeding of the IXth International Congress of Virology, Glasgow 1993, p 335

  2. Bevan M (1984) Binary vectors for plant transformation. Nucleic Acids Res 12:8711–8721

    Google Scholar 

  3. Breathnach R, Chambon P (1981) Organization and expression of eucaryotic split genes coding for proteins. Annu Rev Biochem 50: 349–383

    Google Scholar 

  4. Briddon RW, Pinner MS, Stanley J, Markham PG (1990) Geminivirus coat protein gene replacement alters insect specificity, Virology 177: 85–94

    Google Scholar 

  5. Czosnek H, Navot N, Laterrot H (1990) Geographical distribution of tomato yellow leaf curl virus. A first survey using a specific DNA probe. Phytopathol Medit 29: 1–6

    Google Scholar 

  6. Davies JW, Stanley J (1989) Geminivirus genes and vectors. Trends Gen 5: 77–81

    Google Scholar 

  7. Dry IB, Rigden JE, Krake LR, Mullineaux PM, Rezaian MA (1993) Nucleotide sequence and genome organization of tomato leaf curl geminivirus. J Gen Virol 74: 147–151

    Google Scholar 

  8. Feng DF, Johnson MS, Doolittle RF (1985) Aligning amino acid sequences: comparison of commonly used methods. J Mol Evol 21: 112–125

    Google Scholar 

  9. Grimsley N, Bisaro DM (1987) In: Hohn T, Schell J (eds) Plant DNA infectious agents. Springer, Wien New York, pp 87–107

    Google Scholar 

  10. Hamilton WDO, Stein VE, Coutts RHA, Buck KW (1984) Complete nucleotide sequence of the infectious cloned DNA components of tomato golden mosaic virus: potential coding regions and regulatory sequences. EMBO J 3: 2197–2204

    Google Scholar 

  11. Höfgen R, Willmitzer L (1988) Storage of competent cells forAgrobacterium transformation. Nucleic Acids Res 16: 9877

    Google Scholar 

  12. Kheyr-Pour A, Bendahmane M, Matzeit N, Accotto GP, Crespi S, Gronemborn B (1991) Tomato yellow leaf curl virus from Sardinia is a whitefly-transmitted geminivirus. Nucleic Acids Res 19: 6763–6769

    Google Scholar 

  13. Lazarowitz SG (1987) The molecular characterization of geminiviruses. Plant Mol Biol 4: 177–192

    Google Scholar 

  14. Matthews REF (1991) Plant virology, 3rd edn. Academic Press, New York

    Google Scholar 

  15. Messing J, Geraghty D, Heidecker G, Hu NT, Kridl J, Rubenstein I (1983) Plant gene structure. In: Kasuge T, Meredith CP, Hollander A (eds) Genetic engineering of plants. Plenum Press, New York, pp 211–227

    Google Scholar 

  16. Moriones E, Arnó J, Accotto GP, Noris E, Cavallarin L (1993) First report of tomato yellow leaf curl virus in Spain. Plant Dis 77: 953

    Google Scholar 

  17. Morris B, Coates L, Lowe S, Richardson K, Eddy P (1990) Nucleotide sequence of the infectious cloned DNA components of African cassava mosaic virus (Nigerian strain). Nucleic Acids Res 18: 197–198

    Google Scholar 

  18. Navot N, Pichersky E, Zeidan M, Zamir D, Czosnek H (1991) Tomato yellow leaf curl virus: A whitefly-transmitted geminivirus with a single genomic component. Virology 185: 151–161

    Google Scholar 

  19. Rochester DE, Kositratana W, Beachy RN (1990) Systemic movement and symptom production following agroinoculation with a single DNA of tomato yellow leaf curl geminivirus (Thailand), Virology 178: 520–526

    Google Scholar 

  20. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: A laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor

    Google Scholar 

  21. Sänger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74: 5463–5467

    Google Scholar 

  22. Stanley J (1985) The molecular biology of geminiviruses. Adv Virus Res 30: 139–177

    Google Scholar 

  23. Stanley J, Gay MR (1983) Nucleotide sequence ofcassava latent virus DNA. Nature 301: 260–262

    Google Scholar 

  24. Vieira J, Messing J (1987) Production of single-stranded plasmid DNA. Methods Enzymol 153: 3–11

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Istituto di Fitovirologia Applicata, C.N.R., Torino, Italy

    E. Noris & G. P. Accotto

  2. U.D.L.-I.R.T.A., E.T.S.E. Agrària, Lérida

    E. Hidalgo

  3. Departamento de Patología Vegetal, Institut de la Recerca i Tecnologia Agroalimentàries (I.R.T.A.), Centro de Cabrils, Cabrils, Barcelona, Spain

    E. Moriones

Authors
  1. E. Noris
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. E. Hidalgo
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. G. P. Accotto
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. E. Moriones
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

The nucleotide sequence reported in this paper has been deposited in the EMBL/GenBank and DDBJ Nucleotide Sequence Databases under the accession number Z25751.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Noris, E., Hidalgo, E., Accotto, G.P. et al. High similarity among the tomato yellow leaf curl virus isolates from the West Mediterranean Basin: the nucleotide sequence of an infectious clone from Spain. Archives of Virology 135, 165–170 (1994). https://doi.org/10.1007/BF01309774

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01309774

Keywords

Search

Navigation