Abstract
The α2 subunit of the VLA-2 receptor (CD49B) was mapped to human chromosome 5 by several independent approaches. First, the expression of the α2 subunit at the protein level was investigated in a panel of human-mouse hybrid cell lines. Cell surface expression was detected by indirect immunofluorescence with monoclonal anti-α2 antibody 12F1. Intracellular α2 antigen was detected by immunostaining of whole cell extracts or of immunoprecipitated 12F1 antigen with the monoclonal antibodies 3H8 and 5C5. Second, the presence of human genomic α2 sequences in the panel of human-mouse hybrids was detected by PCR, using primers derived from the published α2 cDNA sequence. The specificity of the amplification product was shown by direct sequencing. The results of the PCR study were confirmed by amplifying aCD14 gene fragment, known to map to chromosome 5. Finally, in situ hybridization with a3H-labeled 1040-bp cDNA probe, also obtained by PCR, confirmed and refined the localization ofCD49B on chromosome 5 at q23-31.
This is a preview of subscription content, log in via an institution to check access.
Literature cited
Hynes, R.O. (1987).Cell 48549–554.
Hemler, M.E., Huang, C., and Schwarz, L. (1987).J. Biol. Chem. 2623300–3309.
Hemler, M.E., Crouse, C., Takada, Y., and Sonnenberg, A. (1988).J. Biol. Chem. 2637660–7665.
Hemler, M.E., Ware, C.F., and Strominger, J.L. (1983).J. Immunol. 131334–340.
Wayner, E.A., and Carter, W.G. (1987).J. Cell Biol. 1051873–1884.
Kunicki, T.J., Nugent, D.J., Staats, S.J., Orchekowski, R.P., Wayner, E.A., and Carter, W.G. (1988).J. Biol. Chem. 2634516–4519.
Staatz, W.D., Rajpara, S.M., Wayner, E.A., Carter, W.G., and Santoro, S.A. (1989).J. Cell Biol. 1081917–1924.
Languino, L.R., Gehlsen, K.R., Wayner, E., Carter, W.G., Engvall, E., and Ruoslahti, E. (1989).J. Cell Biol. 1092455–2462.
Zhang, Y., Saison, M., Spaepen, M., De Strooper, B., Van Leuven, F., David, G., Van den Berghe, H., and Cassiman, J.J. (1988).Somat. Cell Mol. Genet. 1499–104.
Goodfellow, P.J., Nevanlinna, H.A., Gorman, P., Sheer, D., and Lam, G. (1989).Ann. Hum. Genet. 5315–22.
Solomon, E., Palmer, R.W., Hing, S., and Law, S.K.A. (1988).Ann. Hum. Genet. 52123–128.
Bray, P.F., Barsh, G., Rosa, J.P., Luo, X.Y., Magenis, E., and Shuman, M.A. (1988).Proc. Natl. Acad. Sci. U.S.A. 858683–8687.
Rosa, J.P., Bray, P.F., Gayet, O., Johnston, G.I., Cook, R.G., Jackson, K.W., Shuman, M.A., and McEver, R.P. (1988).Blood 72593–600.
Corbi, A.L., Larson, R.S., Kishimoto, T.K., Springer, T.A., and Morton, C.C. (1988).J. Exp. Med. 1671597–1607.
Bray, P.F., Rosa, J.P., Johnston, G.I., Shiu, D.T., Cook, R.G., Lau, C., Kan, Y.W., McEver, R.P., and Shuman, M. (1987).J. Clin. Invest. 801812–1817.
Sosnoski, D., Emanuel, B., Nussbaum, R.L., Hawkins, A.L., Van Tuinen, D., Kao, F.T., Schwartz, E., Phillips, D., Bennett, J.S., Fitzgerald, L.A., and Poncz, M. (1988).J. Clin. Invest. 811993–1998.
Kantor, R.S., Mattes, M.J., Lloyd, K.O., Old, L.J., and Albino, A.P. (1987).J. Biol. Chem. 26215158–15165.
Rettig, W.J., Dracopoli, N.C., Goetzger, T.A., Spengler, B.A., Bielder, J.L., Oettgen, H.F., and Old, L.J. (1984).Proc. Natl. Acad. Sci. U.S.A. 816437–6441.
Peters, P.M., Kamarck, M.E., Hemler, M.E., Strominger, J.L., and Ruddle, F.H. (1984).J. Exp. Med. 1591441–1454.
Pignatelli, M., and Bodmer, W.F. (1988).Proc. Natl. Acad. Sci. U.S.A. 855561–5565.
De Strooper, B., Saison, M., Jaspers, M., Spaepen, M., Van Leuven, F., Van den Berghe, H., and Cassiman, J.J. (1988).Cell Biol. Int. Rep. 129–16.
Pischel, K.D., Hemler, M.E., Huang, C., Bluestein, H.G., and Woods, V.L. (1987).J. Immunol. 138226–233.
Saiki, R.K., Gelfand, D.H., Stoffel, S., Scharf, S.J., Higuchi, R., Horn, G.T., Mullis, K.B., and Erlich, H.A. (1988).Science 239487–491.
Takada, Y., and Hemler, M.E. (1989).J. Cell Biol. 109397–407.
Ferrero, E., and Goyert, S.M. (1988).Nucleic Acids Res. 164173–4173.
Goyert, S.M., Ferrero, E., Rettig, W.J., Yenamandra, A.K., Obara, F., and Le Beau, M.M. (1988).Science 239497–500.
Matsuura, K., Sekoguchi, M., Nasu, N., Higuchi, Y., Yoshida, S., Akizuki, S., and Yamamoto, S. (1989).Nucleic Acids Res. 172132–2132.
Maniatis, T., Fritsch, E.F., and Sambrook, J. (1982). (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York).
Denhardt, D.T. (1966).Biochem. Biophys. Res. Commun. 23641–646.
Cuppens, H., Marynen, P., De Boeck, C., De Baets, F., Eggermont, E., Van den Berghe, H., and Cassiman, J.J. (1990).J. Med. Genet. 27717–719.
Marynen, P., Zhang, J., Cassiman, J.J., Van den Berghe, H., and David, G. (1989).J. Biol. Chem. 2647017–7024.
Donlon, T.A. (1986).Karyogram 123–10.
Harper, M.E., and Saunders, G.F. (1981).Chromosoma 83431–439.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Jaspers, M., Marynen, P., Aly, M.S. et al. Localization of the gene encoding the α2 subunit of the human VLA-2 receptor to chromosome 5q23-31. Somat Cell Mol Genet 17, 505–511 (1991). https://doi.org/10.1007/BF01233174
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF01233174