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Phytochrome inhibits the effectiveness of gibberellins to induce cell elongation in rice

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Abstract

Red light controls cell elongation in seedlings of rice (Oryza sativa L.) in a far-red-reversible manner (Nick and Furuya, 1993, Plant Growth Regul.12, 195–206). The role of gibberellins and microtubules in the transduction of this response was investigated in the rice cultivars Nihon Masari (japonica type) and Kasarath (indica type). The dose dependence of mesocotyl elongation on applied gibberellic acid (GA3) was shifted by red light, and this shift was reversed by far-red light. In contrast, coleoptile elongation was found to be independent of exogenous GA3. Nevertheless, it was inhibited by red light, and this inhibition was reversed by far-red light. The content of the active gibberellin species GA1 and GA4 was estimated by radio-immunoassay. In the mesocotyl, the gibberellin content per cell was found to increase after irradiation with red light, and this increase was far-red reversible. Conversely, the cellular gibberellin content injaponica-type coleoptiles did not exhibit any significant light response. Microtubules reoriented from transverse to longitudinal arrays in response to red light and this reorientation could be reversed by subsequent far-red light in both the coleoptile and the mesocotyl. This movement was accompanied by changes in cell-wall birefringence, indicating parallel reorientations of cellulose deposition. The data indicate that phytochrome regulates the sensitivity of the tissue towards gibberellins, that gibberellin synthesis is controlled in a negative-feedback loop dependent on gibberellin effectiveness, and that at least two hormone-triggered signal chains are linked to the cytoskeleton in rice.

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Abbreviations

D:

darkness

FR:

far-red light

GA3 :

gibberellic acid

GC-SIM:

gas chromatography-selected ion monitoring

R:

red light

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Authors and Affiliations

  1. Department of Agricultural Chemistry, University of Tokyo, Bunkyo-ku, 113, Tokyo, Japan

    Tomonobu Toyomasu & Noboru Murofushi

  2. Biotechnology Research Center, University of Tokyo, Bunkyo-ku, 113, Tokyo, Japan

    Hisazaku Yamane

  3. CNRS-IBMP, 12 rue du Général Zimmer, 67084, Strasbourg, France

    Peter Nick

Authors
  1. Tomonobu Toyomasu
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  2. Hisazaku Yamane
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  3. Noboru Murofushi
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  4. Peter Nick
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Additional information

This work was supported by a grant of the Human Frontier Science Organization to P.N. Advice and organizational support by Prof. M. Furuya (Hitachi Advanced Research Laboratory, Hatoyama, Japan) and Prof. N. Murofushi (Department of Agricultural Chemistry, University of Tokyo, Japan) is gratefully acknowledged. Seeds of both rice cultivars were kindly provided by Dr. O. Yatou (Institute for Radiation Breeding, Hitachi-Ohmiya, Japan), and the antiGA1 Me-antiserum for the radio-immunoassays by Dr. I. Yamaguchi (Department of Agricultural Chemistry, University of Tokyo, Japan).

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Toyomasu, T., Yamane, H., Murofushi, N. et al. Phytochrome inhibits the effectiveness of gibberellins to induce cell elongation in rice. Planta 194, 256–263 (1994). https://doi.org/10.1007/BF01101686

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  • DOI: https://doi.org/10.1007/BF01101686

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