Summary
Prolonged fixation times and handling procedures designed to minimize the introduction of post mortem artifact were used in preparation of rat trigeminal ganglia for fine structural study. Larger, more numerous, light neurons and smaller, less numerous, dark neurons were observed. Major fine structural differences included a more discrete clumping of Nissl substance and more neurofilaments in the light compared with the dark cell. The results suggest that these differences are not artifacts of preparation.
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References
Angeletti, P. U., Levi-Montalcini, R. andCaramia, F. (1971) Ultrastructural changes in sympathetic neurons of newborn and adult mice treated with nerve growth factor.Journal of Ultrastructure Research 36, 24–36.
Bunge, M. B., Bunge, R. P., Peterson, E. P. andMurray, M. R. (1967) A light and electron microscope study of long term organized cultures of rat dorsal root ganglia.Journal of Cell Biology 32, 439–66.
Cammermeyer, J. (1962) An evaluation of the significance of the ‘dark’ neuron.Ergebnisse der Anatomie und Entwicklungsgeschichte 36, 1–61.
Crosby, E. C., Humphrey, T. andLauer, E. W. (1962)Correlative Anatomy of the Nervous System. New York: The Macmillan Company.
Dawson, I. M., Hossack, J. andWyburn, G. M. (1955) Observations on the Nissl's substance, cytoplasmic filaments and the nuclear membrane of spinal ganglion cells.Proceedings of the Royal Society of London, Series B144, 132–42.
Dixon, A. D. (1963a) Fine structure of nerve-cell bodies and satellite cells in the trigeminal ganglion.Journal of Dental Research 42, 990–9.
Dixon, A. D. (1963b) The ultrastructure of nerve fibers in the trigeminal ganglion of the rat.Journal of Ultrastructure Research 8, 107–121.
Fawcett, D. W. (1966)The Cell. An atlas of fine structure. Philadelphia and London: W. B. Saunders.
Hess, A. (1955) The fine structure of young and old spinal ganglia.Anatomical Record 123, 399–423.
Johnston, M. C. andHazelton, R. D. (1972) Embryonic origins of facial structures related to oral sensory and motor functions. InOral Sensation and Perception (edited by Bosma, J. F.) Chapter 4. Springfield, Illinois: Charles C. Thomas.
Kalina, M. andBubis, J. J. (1968) Histochemical studies on the distribution of acid phosphatases in neurones of sensory ganglia; light and electron microscopy.Histochemie 14, 103–12.
Kalina, M. andWolman, M. (1970) Correlative histochemical and morphological study on the maturation of sensory ganglion cells in the rat.Histochemie 22, 100–8.
Karnovsky, M. J. (1965) A formaldehyde-glutaraldehyde fixative of high osmolality for use in electron microscopy.Journal of Cell Biology 27, 137A-138A.
Levi-Montalcini, R., Caramia, F., Luse, S. A. andAngeletti, P. U. (1968)In vitro effects of the nerve growth factor on the fine structure of sensory nerve cells.Brain Research 8, 347–62.
Matsuura, H., Mori, M. andKawakatsu, K. (1969) A histochemical and electron-microscopic study of the trigeminal ganglion of the rat.Archives of Oral Biology 14, 1135–46.
Moses, H. L. (1967) Comparative fine structure of the trigeminal ganglia, including human autopsy studies.Journal of Neurosurgery 26, 112–26.
Novikoff, A. B. (1967) Enzyme localization and ultrastructure of neurons. InThe Neuron (edited by Hydén, H.), pp. 255–318. Amsterdam-London-New York: Elsevier.
Novikoff, P. M., Novikoff, A. B., Quintana, N. andHauw, J. J. (1971) Golgi apparatus, GERL, and lysosomes of neurons in rat dorsal root ganglia studied by thick section and thin section cytochemistry.Journal of Cell Biology 50, 859–86.
Palay, S. L., Mcgee-Russell, S. M., Gordon, S. andGrillo, M. S. (1962) Fixation of neural tissues for electron microscopy by perfusion with solutions of osmium tetroxide.Journal of Cell Biology 12, 385–410.
Peach, R. (1969) The existence of light and dark cells within the trigeminal ganglion.Anatomical Record 163, 319–20.
Peach, R. (1970a) Simple apparatus for vascular perfusion of Fixative for electron microscopy.Journal of Dental Research 49, 891.
Peach, R. (1970b) Fixative osmolality and the fine structure of the trigeminal ganglion.Journal of Dental Research 49 Supplement, 210.
Pease, D. C. (1964)Histological techniques for electron microscopy. Second edition, pp. 234–6, New York and London: Academic Press.
Peters, A., Palay, S. L. andWebster, H. deF. (1970)The fine structure of the nervous system: The cells and their processes, p. 8, New York and London: Harper and Row.
Pineda, A., Maxwell, D. S. andKruger, L. (1967) The fine structure of neurons and satellite cells in the trigeminal ganglion of cat and monkey.American Journal of Anatomy 121, 461–88.
Reynolds, E. S. (1963) The use of lead citrate at high pH as an electron-opaque stain in electron microscopy.Journal of Cell Biology 17, 208–12.
Richardson, K. C., Jarett, L. andFinke, E. H. (1960) Embedding in epoxy resins for ultrathin sectioning in electron microscopy.Stain Technology 35, 313–23.
Tennyson, V. M. (1965) Electron microscopic study of the developing neuroblast of the dorsal root ganglion of the rabbit embryo.Journal of Comparative Neurology 124, 267–318.
Tennyson, V. M. (1969) The fine structure of the developing nervous system. InDevelopmental Neurobiology (edited by Himwich, W.), Part 2, Chapter 3. Springfield, Illinois: Charles C. Thomas.
Weston, J. (1970) The migration and differentiation of neural crest cells.Advances in Morphogenesis 8, 41–144.
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Peach, R. Fine structural features of light and dark cells in the trigeminal ganglion of the rat. J Neurocytol 1, 151–160 (1972). https://doi.org/10.1007/BF01099181
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DOI: https://doi.org/10.1007/BF01099181