Summary
Cells of the osmophilic yeastPichia farinosa were immobilized in sintered glass Raschig rings for the production of glycerol. The kinetics of production were observed under different conditions in batch, fed-batch and semicontinuous fermentations in fixed-bed column reactors and compared with those of free cells. 2.6 × 109 cells/g sintered glass were adsorbed. The glycerol productivity amounted to 8.1 g/l per day. The highest concentration reached in batch culture was 86 g/l with immobilized cells. Fermentations using immobilized cells were accelerated compared to fermentations using free cells and maximum yield and productivity were reached at lower initial sugar concentrations. Using scanning electron microscopy it was observed that the shape of the cells was related to the sugar concentration in the medium. The experiments show thatP. farinosa produces glycerol with a high and constant productivity over long periods of time.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Aivasidis A (1984) Scale up of new fixed bed loop reactor for anaerobic digestion. In: Third European Congress on Biotechnology, vol III. Verlag Chemie, Weinheim, pp 51–60
Bandyopadhyay KK, Ghose TK (1983) Studies on immobilizedSaccharomyces cerevisiae. III. Physiology of growth and metabolism on various supports. Biotechnol Bioeng 24:805–813
Baumann U (1988) Glycerinbildung mit immobilisierten osmophilen Hefen. Diploma Thesis. Institut für Mikrobiologie an der Westfälischen Wilhelms-Univeristät, Münster
Bisping B, Rehm HJ (1986) Glycerol production by cells ofSaccharomyces cerevisiae immobilized in sintered glass. Appl Microbiol Biotechnol 32:174–179
Bisping B, Hecker D, Rehm HJ (1989) Glycerol production by semicontinuous fed-batch fermentation with immobilized cells ofSaccharomyces cerevisiae. Appl Microbiol Biotechnol 32:119–123
Brown AD, Mackenzie KF, Singh KK (1986) Selected aspects of microbial osmoregulation. FEMS Microbiol Rev 39:31–36
Eoff JR, Lindner WV, Beyer GF (1919) Production of glycerin from sugar by fermentation. J Ind Eng Chem 11:842–845
Hilge B, Rehm HJ (1989) Comparison of metabolism and macromolecular composition of free and in Ca alginate entrappedSaccharomyces cerevisiae. In: Proceedings of the 7th Dechema Jahrestagung der Biotechnologen/Joint Meeting of SIM and Dechema. Dechema Biotechnology monographs
Honecker S, Bisping B, Zhu Yang, Rehm HJ (1989) Influence of sucrose concentration and phosphate limitation on citric acid production by immobilized cells ofAspergillus niger. Appl Microbiol Biotechnol 31:17–24
Mattiasson B, Hahn-Hägerdall B (1982) Microenvironmental effects on metabolic behavior of immobilized cells: a hypothesis. Eur J Appl Microbiol Biotechnol 16:52–55
Navarro JM, Durand G (1977) Modification of yeast metabolism by immobilization onto porous glass. Eur J Appl Microbiol Biotechnol 4:243–254
Pfeiffer P, Radler F (1985) Hochleistungsflüssigkeitschromato-ographische Bestimmung von organischen Säuren, Zuckern, Glycerin und Alkohol in Wein an einer Kationenaustauschersäule. Z Lebensm Unters Forsch 181:24–27
Rehm HJ (1988) Microbial production of glycerol and other polyols. In: Rehm HJ, Reed G (eds) Biotechnology, vol 6b. VCH Verlagsgesellschaft, Weinheim, pp 51–69
Spencer JFT, Roxburgh JM, Sallans HR (1957) Factors influencing the production of polyhydric alcohols by osmophilic yeasts. J Agric Food Chem 5:64–67
Vijaikishore P, Karanth NK (1984) Glycerol production from glucose in alkaline medium. Biotechnol Lett 6:103–108
Vijaikishore P, Karanth NK (1986) Factors affecting glycerol production byPichia farinosa under alkaline conditions. Appl Biochem Biotechnol 13:189–205
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Bisping, B., Baumann, U. & Rehm, H.J. Production of glycerol by immobilizedPichia farinosa . Appl Microbiol Biotechnol 32, 380–386 (1990). https://doi.org/10.1007/BF00903769
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00903769