Summary
The degradation of 4-chlorophenol by free and by Ca-alginate-immobilized cells ofAlcaligenes sp. A 7-2 has been studied. Increasing concentrations of 4-chlorophenol (0.4–0.55 mM) were better tolerated and more quickly degraded by the immobilized organisms than by free cells. The capability for haloarene-degradation is inducible. In semicontinuous fermentation at pH 7 a minimal degradation time of 5 h for degrading 0.2 mM 4-chlorophenol was reached. Fermentation temperature was shown to be important for inducing the degradation capability, but to be less important for the degradation rate by induced organisms. High-frequency feeding of small amounts of 4-chlorophenol (0.05 mM) was more favourable than low-frequency feeding of larger amounts (0.15 mM).
Continuous fermentation with unbuffered medium allowed a degradation rate of about 2 mmol·l-1·d-1; with buffered medium a higher degradation rate of nearly 4 mmol·l-1·d-1 was reached, but the Ca-alginate beads dissolved.
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Westmeier, F., Rehm, H.J. Biodegradation of 4-chlorophenol by entrappedAlcaligenes sp. A 7-2. Appl Microbiol Biotechnol 22, 301–305 (1985). https://doi.org/10.1007/BF00582412
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DOI: https://doi.org/10.1007/BF00582412