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The growth of Spirillum volutans ehrenberg in mixed and pure cultures

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Summary

The growth of Spirillum volutans in mixed culture was studied and liquid media were developed in which it grew rapidly and attained populations of over a million cells per ml. By taking advantage of their rapid and unidirectional motility, spirilla were separated from the mixed population by migration in capillary tubes. Pure cultures were achieved by growing the separated spirilla inside of dialysis sacks in contact with mixed cultures on the outside, and also by growth in an asparagine-mineral salts medium supplemented with an extract of Escherichia coli.

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Authors and Affiliations

  1. Hopkins Marine Station, Stanford University, Pacific Grove, California

    Beatrice T. Rittenberg & Sydney C. Rittenberg

  2. the Department of Bacteriology, University of Southern California, Los Angeles, California

    Beatrice T. Rittenberg & Sydney C. Rittenberg

Authors
  1. Beatrice T. Rittenberg
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  2. Sydney C. Rittenberg
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Additional information

Dedicated to Professor E. G. Pringsheim on his 80th birthday.

This investigation was supported in part by grant G 9882 from the National Science Foundation.

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Rittenberg, B.T., Rittenberg, S.C. The growth of Spirillum volutans ehrenberg in mixed and pure cultures. Archiv. Mikrobiol. 42, 138–153 (1962). https://doi.org/10.1007/BF00408170

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  • DOI: https://doi.org/10.1007/BF00408170

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