Summary
Infection of E. coli with the viruses T7 or T3 leads to a dramatic efflux of potassium ions. This ion efflux is caused by the virus particle since no concomitant protein synthesis is required. T7 mutants carrying deletions in the M-gene (Schweiger et al., 1975), however, yield virus particles disturbed in the ion release.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bhattacharyya, P., Epstein, W., Silver, S.: Valinomycin-induced uptake of potassium in membrane vesicles from Escherichia coli. Proc. nat. Acad. Sci. (Wash.) 68, 1488–1492 (1971)
Britton, J.R., Haselkorn, R.: Permeability lesions in male Escherichia coli infected with bacteriophage T7. Proc. nat. Acad. Sci. (Wash.) 72, 2222–2226 (1975)
Chamberlin, M., Ring, J.: Characterization of T7-specific ribonucleic acid polymerase. II. Inhibitors of the enzyme and their application to the study of the enzymatic reaction. J. biol. Chem. 248, 2245–2250 (1973)
Condit, R.C.: F factor-mediated inhibition of bacteriophage T7 growth: Increased membrane permeability and decreased ATP levels following T7 infection of male Escherichia coli. J. molec. Biol. 98, 45–56 (1975)
Hantke, K., Braun, V.: Fluorescence studies on first steps of phage-host interaction. Virology 58, 310–312 (1974)
Herrlich, P., Rahmsdorf, H.J., Pai, S.H., Schweiger, M.: Translational control induced by bacteriophage T7. Proc. nat. Acad. Sci. (Wash.) 71, 1088–1092 (1974)
Herrlich, P., Rahmsdorf, H.J., Schweiger, M.: Regulation of macromolecular synthesis by membrane changes. Advances in the Biosciences, Vol. 12, pp. 523–537. Braunschweig: Pergamon Press 1974
Herrlich, P., Schweiger, M.: DNA- and RNA-directed synthesis in vitro of phage enzymes. Methods in Enzymology 30, 654–669 (1974)
Hill, R.F.: A radiation sensitive mutant of Escherichia coli. Biochim. biophys. Acta (Amst.) 30, 636–637 (1958)
Lechner, S.: Isolierung und Characterisierung eines neuen Endolysins (Bakteriophage T7). Master Thesis Technische Universität Berlin, 1975
Lester, G.: Requirement for potassium by bacteria. J. Bact. 75, 426–428 (1958)
Lombardi, F.J., Reeves, J.P., Kaback, H.R.: Mechanisms of active transport in isolated bacterial membrane vesicles. XIII. Valinomycin-induced rubidium transport. J. biol. Chem. 248, 3551–3565 (1973)
Mathews, C.K.: Bacteriophage biochemistry. ACS Monograph, Van Nostrand Reinhold Company 1971
Pai, S.H., Rahmsdorf, H.J., Ponta, H., Hirsch-Kauffmann, M., Herrlich, P., Schweiger, M.: Protein kinase of bacteriophage T7. 2. Properties, enzyme synthesis in vitro and regulation of enzyme synthesis and activity in vivo. Europ. J. Biochem. 55, 305–314 (1975)
Ponta, H., Pon, C.L., Herrlich, P., Gualerzi, C., Hirsch-Kauffmann, M., Pfennig-Yeh, M., Rahmsdorf, H.J.: The sex-factor dependent exclusion of colivirus T7. Europ. J. Biochem. 59, 261–270 (1975)
Ponta, H., Rahmsdorf, H.J., Pai, S.H., Herrlich, P. Schweiger, M.: Control of gene expression in bacteriophage T7. Isolation of a new control protein and mechanism of action. Molec. gen. Genet. 134, 29–38 (1974a)
Ponta, H., Rahmsdorf, H.J., Pai, S.H., Hirsch-Kauffmann, M., Herrlich, P., Schweiger, M.: Control of gene expression in bacteriophage T7: Transcriptional controls. Molec. gen. Genet. 134, 281–297 (1974b)
Scherzinger, E., Herrlich, P., Schweiger, M.: Transcription of T3 and T7 early genes by T3 and T7 RNA polymerases. Molec. gen. Genet. 118, 67–77 (1972)
Schweiger, M., Herrlich, P., Scherzinger, E., Rahmsdorf, H.J.: Negative control of protein synthesis after infection with bacteriophage T7. Proc. nat. Acad. Sci. (Wash.) 69, 2203–2207 (1972)
Schweiger, M., Hirsch-Kauffmann, M., Ponta, H., Pfennig-Yeh, M., Herrlich, P.: Biochemistry of T7 development. FEBS Symposium 39, 55–68 (1975)
Studier, F.W.: Gene 0.3 of Bacteriophage T7 acts to overcome the DNA restriction system of the host. J. molec. Biol. 94, 283–295 (1975)
Weiden, P.L., Epstein, W., Schultz, S.G.: Cation transport in Escherichia coli. VII Potassium requirement for phosphate uptake. J. gen. Physiol. 50, 1641–1661 (1967)
Wood, W.B.: Host specificity of DNA produced by Escherichia coli: Bacterial mutations affecting the restriction and modification of DNA. J. molec. Biol. 16, 118–133 (1966)
Author information
Authors and Affiliations
Additional information
Communicated by E. Bautz
Rights and permissions
About this article
Cite this article
Ponta, H., Altendorf, KH., Schweiger, M. et al. E. coli membranes become permeable to ions following T7-virus-infection. Molec. gen. Genet. 149, 145–150 (1976). https://doi.org/10.1007/BF00332882
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00332882