Abstract
The fluorescence polarization levels of liver cell membranes and plasma were analyzed to determine membrane fluidity following bile duct ligation (BDL) in rats. Fluorescence polarization was measured with a spectrofluorophotometer equipped with polarizers, using 1,6-diphenyl-1,3,5-hexatrien (DPH) as a probe. After bile duct ligation, liver cell membrane fluidity decreased significantly for up to 14 days after surgery (P<0.001 on 3rd and 7th days). The polarization of the plasma in rats with BDL slightly but significantly increased compared to the levels in the control animals over the 14-day period following BDL. In addition, a small but significant correlation in the polarization levels between plasma and liver cell membranes (r=0.362, P<0.02) was observed. The co-incubation of BDL plasma with normal liver cell membranes resulted in a decrease in membrane fluidity, which suggested that BDL rat plasma had a direct effect on membrane fluidity. After a 70% hepatectomy, the polarization of the membranes from remnant livers in the BDL rats remained elevated relative to the shamoperated controls. It is thus concluded that the membrane fluidity of the livers in BDL rats decreases following bile duct ligation and does not increase after a 70% hepatectomy, presumably due to the increased plasma level of bilirubin.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Doerr RJ, Yildiz I, Flint LM (1990) Pancreaticoduodenectomy. Arch Surg 125:463–465
Bengmark S, Ekberg H, Evander A, Klofver-Stahl B, Tranberg KG (1988) Major liver resection for hilar cholangioma. Ann Surg 207:120–125
Tanaka J, Ozawa K, Tobe T (1979) Significance of arterial ketone body ratio as an indicator of hepatic cellular energy status in jaundiced rabbits. Gastroenterol 69:691–696
Schachter D (1984) Fluidity and function of hepatocyte plasma membrane. Hepatology 4:140–151
Portoles MT, Pagani R, Diaz-Laviada I, Municio A (1987) Effect of Escherichia coli lipopolysaccharide on the microviscosity of liver plasma membranes and hepatocyte suspensions and monolayers. Cell Biochemistry and Function 5:55–61
Yoshida M, Tanaka J, Tamura J, Fujita K, Kasamatsu T, Kohmoto M, Tobe T (1993) Altered membrane fluidity of liver plasma membranes following partial hepatectomy in rats. J Surg Res 55:390–396
Anel A, Naval J, Gonzalez B, Torres JM, Mishal Z, Vriel J, Pineiro A (1990) Fatty acid metabolism in human lymphocytes. 1. Time-course changes in fatty acid composition and membrane fluidity during blastic transformation of peripheral blood lymphocytes. Biochim Biophys Acta 1044:323–331
Higgins GM, Anderson RM (1931) Experimental pathology of the liver: 1. restoration of the white rat following partial surgical removal. Arch Pathol 12:186–202
Maeda T, Balakishnar K, Mehdi SQ (1983) A simple and rapid method for the separation of plasma membranes. Biochim Biophys Acta 731:115–120
Michaelsson M (1971) Studies on diazo methods and a new copper azo pigment method. Scand J Clin Lab Invest 1961 [Suppl]:56
Cheng S, Levy D (1979) The effects of cell proliferation on the lipid composition and fluidity of hepatocytes plasma membranes. Arch Biochem Biophys 196:424–429
Couger ML (1971) Mechanism of bilirubin toxicity on tissue culture cells. Biochem Med 5:1–16
Zetterstrom REL (1956) Bilirubin, an uncoupler of oxidative phosphorylation in isolated mitochondria. Nature 174:1335–1337
Thaler MM (1971) Bilirubin toxicity in hepatoma cells. Nature 230:218–223
Singer SJ, and Nicolson GL (1972) The fluid mosaic model of the structure of cell membranes. Science 175:720–731
Boelsterli UA, Rakhit G, Balazs T (1983) Modulation by S-adenosyl-L-methionine of hepatic Na,K-ATPase, membrane fluidity, and bile flow in rats with ethinyl estradiol-induced cholestasis. Hepatology 3:12–17
Judith S, David S (1984) Dietary induction of acyl chain desaturates alters the lipid composition and fluidity of rat hepatocyte plasma membranes. Biochemistry 23:1165–1170
Tarashi TF, Rubin E (1985) Effects of ethanol on the chemical and structural properties of biologic membranes. Lab Invest 52:120–128
Lee AG (1991) Lipid and their effects on membrane proteins: Evidence against a role for fluidity. Prog Lipid Res 30:323–348
Bodersen R (1979) Bilirubin. Solubility and interaction with albumin and phospholipid. J Biol Chem 254:2364–2369
Berlin E, Judd JT, Nair PP, Jones DY, Taylor PR (1991) Dietary fat and hormone influences on lipoprotein fluidity and composition in premenopausal women. Atherosclerosis 86:95–110
Talafant E (1971) Bile pigment-phospholipid interactions. Biochim Biophys Acta 231:394–399
Katz S, Grosfeld JL, Gross K, Plager DA, Ross D, Rosenthal RS, Hull M, Weber TR (1984) Impaired bacterial clearance and trapping in obstructive jaundice. Ann Surg 199:14–20
Yamamoto M, Yamada T, Ida T, Ozawa K (1977) Inhibitory effect of jaundice on regeneration. Res Exp Med 171:121–128
Himeno Y, Engelman RW, Good RA (1992) Influence of calorie restriction on oncogene expression and DNA synthesis during liver regeneration. Proc Natl Acad Sci USA 89:5497–5501
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Zhong, W.B., Tanaka, J., Komoto, M. et al. Alteration in the fluorescence polarization of rat plasma and liver cell membranes following bile duct ligation in rats. Surg Today 25, 536–541 (1995). https://doi.org/10.1007/BF00311311
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00311311