Abstract
A wild isolate of Penicillium canescens was subjected to mutagenesis, and 150 chlorate-resistant mutants were isolated and classified in respect of their ability to utilize various nitrogen sources. Strains supposedly deficient in nitrate reductase have been transformed with the nitrate-reductase gene from Aspergillus niger. Transformation probably occurred by non-homologous integration of the transforming vector into the chromosome. Co-transformation with the AMA1 replicating element from A. nidulans enhanced transformation frequency up to 2000-fold, and was shown to result in autonomous maintenance of replicating concatenates, one of which was re-isolated by transformation of E. coli.
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Communicated by H. T. Jacobs
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Aleksenko, A.Y., Makarova, N.A., Nikolaev, I.V. et al. Integrative and replicative transformation of Penicillium canescens with a heterologous nitrate-reductase gene. Curr Genet 28, 474–477 (1995). https://doi.org/10.1007/BF00310818
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DOI: https://doi.org/10.1007/BF00310818