Abstract
Somatic embryogenesis and plant regeneration were obtained from immature leaflet callus of chickpea. Numerous globular embryos developed on the surface of callus on Murashige and Skoog's (1962) medium containing 25 μM 2,4-dichlorophenoxyacetic acid. These globular embryos differentiated into mature somatic embryos upon removal of 2,4-dichlorophenoxyacetic acid. The maturation of embryos was significantly affected by pH, photoperiod, abscisic acid and genotype. Callus continued to produce somatic embryos for over 8 subcultures at 4 week intervals. Two per cent of the embryos formed plants on medium containing 15 μM gibberellic acid and 1 μM indole-3-butyric acid. Desiccation of embryos for a period of 3 d increased their rate of conversion into plants from 0.9 to 2.8%. All regenerated plants showed normal morphological characteristics.
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Abbreviations
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
- NAA:
-
1-naphthaleneacetic acid
- Kn:
-
Kinetin
- BA:
-
6-benzyladenine
- TDZ:
-
Thidiazuron
- GA3 :
-
Gibberellic acid
- IBA:
-
Indole-3-butyric acid
- ABA:
-
Abscisic acid
- AC:
-
Activated charcoal
- AgNO3 :
-
Silver nitrate
- PVP:
-
Polyvinylpyrrolidone-40,000 mol wt
- MS:
-
Murashige and Skoog (1962) medium
References
Altaf N, Ahmad MS (1990) In: Bajaj YPS (ed) Biotechnology in Agriculture and Forestry, Vol 10, Legumes and Oilseed Crops 1, Springer-Verlag, Berlin Heidelberg pp 100–113
Attree SM, Tautorus TE, Dunstan DI, Fowke LC (1990) Can J Bot 68:2583–2589
Chen THH, Marowitch J (1987) J Plant Physiol 128:271–277
Duncan DB (1955) Biometrics 11:1–42
Hakman I, von Arnold S (1988) Physiol Plant 72:579–587
Johansen DA (1940) Plant Microtechnique. Tata McGraw Hill Publishing Company Ltd, Bombay New Delhi
Komatsuda T, Ohyama K (1988) Theor Appl Genet 75:695–700
Kysely W, Jacobsen HJ (1990) Plant Cell Tiss Org Cult 20:7–14
Murashige T, Skoog F (1962) Physiol Plant 15:473–497
Parrott WA, Dryden G, Vogt S, Hildebrand DF, Collins GB, Williams EG (1988) In Vitro Cell Devel Biol 24:817–820
Rao BG, Chopra VL (1989) J Plant Physiol 134:637–638
Rashid A, Reinert J (1983) Protoplasma 116:155–160
Sellars RM, Southward GM, Phillips GC (1990) Crop Sci 30:408–414
Smith DL, Krikorian AD (1990) Amer J Bot 77:1634–1647
Snedecor GW, Cochran WG (1968) Statistical Methods. Oxford and IBH Publishing Co Pvt Ltd, New Delhi Bombay Calcutta
Tomar UK, Gupta SC (1988) Plant Cell Reports 7:70–73
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Communicated by G. C. Phillips
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Barna, K.S., Wakhlu, A.K. Somatic embryogenesis and plant regeneration from callus cultures of chickpea (Cicer arietinum L.). Plant Cell Reports 12, 521–524 (1993). https://doi.org/10.1007/BF00236100
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DOI: https://doi.org/10.1007/BF00236100