Summary
Suspension cells of Oryza sativa L. (rice) were transformed, by microprojectile bombardment, with plasmids carrying the coding region of the Streptomyces hygroscopicus phosphinothricin acetyl transferase (PAT) gene (bar) under the control of either the 5′ region of the rice actin 1 gene (Act1) or the cauliflower mosaic virus (CaMV) 35S promoter. Subsequently regenerated plants display detectable PAT activity and are resistant to BASTATM, a phosphinothricin (PPT)-based herbicide. DNA gel blot analyses showed that PPT resistant rice plants contain a bar-hybridizing restriction fragment of the expected size. This report shows that expression of the bar gene in transgenic rice plants confers resistance to PPT-based herbicide by suppressing an increase of ammonia in plants after spraying with the herbicide.
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References
Cao J, Wang YC, Klein TK, Sanford J, Wu R (1990) in Plant Gene Transfer (Lamb CJ, Beachy RN, eds.), Wiley-Liss, New York, USA, pp. 21–33
Cao J, Zhang W, McElroy D, Wu R (1991) in Rice Biotechnology (Khush GS, Toenniessen GH, eds.), C.A.B. International, Oxon, UK, pp. 175–198
Christou P, Ford TL, Kofron M (1991) Bio/Tech 9:957–962
Datta SK, Peterhans A, Datta K, Potrykus I (1990) Bio/Tech 8:736–740
Dekeyser R, Claes B, Marichal M, Van Montague M, Caplan A (1989) Plant Physiol 90:217–223
D'Halluin K, De Block M, Denecke J, Janssens J, Leemans J, Reynaerts A, Botterman J (1992) Meth Enzymol 216: in press
Linsmaier EM, Skoog F (1965) Physiol Plant 18:100–127
McElroy D, Zhang W, Cao J, Wu R (1990) Plant Cell 2:163–171
McElroy D, Blowers AD, Jenes B, Wu R (1991) Mol Gen Genet 231:150–160
Shimamoto K, Terada R, Izawa T, Fujimoto H (1989) Nature 338:274–276
Tachibana K, Watanabe T, Sekizawa Y, Takematsu T (1986) J Pest Sci 11:33–37
Thompson CJ, Movva NR, Tizard R, Crameri R, Davies JE, Lauwereys M, Botterman J (1987) EMBO J 6:2519–2523
Toriyama K, Arimoto Y, Uchimiya H, Hinata K (1988) Bio/Tech 6:1072–1074
White J, Chang S-YP, Bibb MJ, Bibb MJ (1990) Nucl Acids Res 18:1062
Wu R, Kemmerer EC, McElroy D (1990) in Gene Manipulation in Plant Improvement II (Gustafson JP, ed.), Plenum Press, NY, pp. 251–263
Yang H, Zhang HM, Davey MR, Mulligan BJ, Cocking EC (1988) Plant Cell Rep 7:421–425
Ye GN, Daniell H, Sanford JC (1990) Plant Mol Biol 15:809–819
Zhang W, Wu R (1988) Theor Appl Genet 76:835–840
Zhang W, McElroy D, Wu R (1991) Plant Cell 3:1155–1165
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Communicated by E. D. Earle
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Cao, J., Duan, X., McEIroy, D. et al. Regeneration of herbicide resistant transgenic rice plants following microprojectile-mediated transformation of suspension culture cells. Plant Cell Reports 11, 586–591 (1992). https://doi.org/10.1007/BF00233098
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DOI: https://doi.org/10.1007/BF00233098