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Beet necrotic yellow vein virus coat protein-mediated protection in sugarbeet (Beta vulgaris L.) protoplasts

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Abstract

Transformed Beta vulgaris L. suspension cultures were obtained after cocultivation of sugarbeet cells with Agrobacterium tumefaciens harbouring a binary vector containing the coat protein gene of beet necrotic yellow vein virus inserted between the kanamycin resistance gene and a ß-glucuronidase reporter gene. Protoplasts were isolated both from untransformed cells, and from transformed cells expressing the viral coat protein, and both were then infected with beet necrotic yellow vein virus. Comparison of the levels of infectivity shows that the expression of the coat protein gene in sugarbeet protoplasts mediates high levels of protection against infection by beet necrotic yellow vein virus.

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Abbreviations

TMV:

Tobacco Mosaic Virus

CP:

Coat Protein

BNYVV:

Beet Necrotic Yellow Vein Virus

ß-Glu:

ß-glucuronidase

MS:

Murashige and Skoog (1962)

PEG:

Polyethylene glycol

npt:

neomycin phosphotransferase

nos:

nopaline synthase

FITC:

fluoresceine isothiocyanate

IAA:

indole acetic acid

BAP:

benzyl amino purine

MES:

2-[N-Morpholino]ethane sulfonic acid

IgG:

Immunoglobulin G

nt:

nucleotide

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Authors and Affiliations

  1. BIOSEM Laboratory, Groupe Limagrain, 24, Avenue des Landais, F-63170, Aubiere, France

    Jean Kallerhoff, Pascual Perez, Sofia Ben Tahar & Joël Perret

  2. I.B.M.P., 12 rue du Général Zimmer, F-67000, Strasbourg, France

    Salah Bouzoubaa

Authors
  1. Jean Kallerhoff
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  2. Pascual Perez
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  3. Salah Bouzoubaa
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  4. Sofia Ben Tahar
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  5. Joël Perret
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Communicated by I. Potrykus

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Kallerhoff, J., Perez, P., Bouzoubaa, S. et al. Beet necrotic yellow vein virus coat protein-mediated protection in sugarbeet (Beta vulgaris L.) protoplasts. Plant Cell Reports 9, 224–228 (1990). https://doi.org/10.1007/BF00232185

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  • DOI: https://doi.org/10.1007/BF00232185

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