Abstract
For optimization of the transformation procedure with Pisum sativum L. stern explant callus was used to test the effect of disarmed Agrobacterium tumefaciens strains, cocultivation procedures (preconditioning of explants; use of Nicotiana tabacum L. nurse cultures), duration of cocultivation (2, 3 or 4 days), and agents for selection (kanamycin or hygromycin). The succinamopine strain EHA101(pBI1042) produced the highest percentage of transformed calli (77%) when used in conjunction with tobacco nurse culture during four days of cocultivation. Using this strain, kanamycin (76%) and hygromycin (77%) were equally effective selective agents, but for strain LBA4404(pBI1042) percentage of transformed calli was higher for hygromycin (63%) than for kanamycin (17%). The procedures and strains shown to be optimal for transformation of pea callus will now be complemented by a pea regeneration system.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bekkaoui F, Datla RSS, Pilon M, Tautoms TE, Crosby WL, Dunstan DI (1990) Theor. Appl. Genet. 79: 353–359.
Chabaud M, Passiatore JE, Cannon F, Buchanan-Wollaston V (1988) Plant Cell Rep. 7: 512–516.
Culianez-Macia FA, Hepburn AK (1988) Plant Mol. Biol. 11: 389–399.
Datla RSS, Hammerlindt J, Crosby WL, Selveraj G (1990) submitted.
Depicker A, Stachel S, Dhaese P, Zambryski P, Goodman HM (1982) Mol. & Applied Gen. 1: 561–573.
Fillati JJ, Kiser J, Rose R, Comai L (1987) Bio/Technology 5: 726–730.
Fraley RT, Rogers SG, Horsch RB, Eichholtz DA, Flick JS, Fink CL, Hoffmann NL, Sanders PR (1985) Bio/Technology 3: 629–635.
Gamborg OL, Miller RA, Ojima K (1968) Exp. Cell Res. 50: 151–158.
Hinchee MAW, Connor-Ward DV, Newell CA, McDonnell RE, Sato SJ, Gasser CS, Fischhoff DA, Re DB, Fraley RT, Horsch RB (1988) Bio/Technology 6: 915–922.
Hobbs SLA, Jackson JA, Mahon JD (1989) Plant Cell Rep. 8: 274–277.
Hobbs SLA, Jackson JA, Baliski DS, DeLong CMO, Mahon JD (1990) Plant Cell Rep. 9: 17–20.
Hood EE, Helmer GL, Fraley RT, Chilton M-D (1986) J. Bacteriol. 168: 1291–1301.
Jackson JA, Hobbs SLA (1990) Plant Cell Rep. in press.
Jefferson RA, Kavanagh TA, Bevan MW (1987) EMBO J 6: 3901–3907.
Koncz C, Schell J (1986) Mol. Gen. Genet. 204: 383–396.
McCormick S, Niedermeyer J, Fry J, Barnason A, Horsch R, Fraley R (1986) Plant Cell Rep. 5: 81–84.
Messing J (1983) Meth. Enzymol. 101: 20–78
Murashige T, Skoog F (1962) Physiol. Plant. 15: 473–497.
Ooms G, Hooykaas PJJ, Moolenaar G, Schilperoot RA (1981) Gene: 14: 33–50.
Peralta EG, Ream W (1985) Proc. Natl. Acad. Sci. USA 82: 5112–5116.
Puonti-Kaerlas J, Stabel P, Eriksson T (1989) Plant Cell Rep. 8: 321–324.
Rogers SG, Horsch RB, Fraley RT (1986) Meth. Enzymol. 118: 627–640.
Rogers SG, Klee HJ, Horsch RB, Fraley RT (1987) Meth. Enzymol. 153: 253–277.
Schenk RU, Hildebrandt AC (1972) Can. J. Bot. 50: 199–204.
Schmidt R, Willmitzer L (1988) Plant Cell Rep. 7: 583–586.
Scott R, Draper J, Jefferson R, Dury G, Jacob L (1988) In: Draper J, Scott R, Armitage P, Walden R (eds) Plant Genetic Transformation and Gene Expression, Blackwell Scientific Pub., Oxford, pp. 263–339.
Staskawicz B, Dahlbeck D, Keen N, Napoli C (1987). J. Bacteriol. 169: 5789–5794.
Vahala T, Stabel P, Eriksson T (1989) Plant Cell Rep. 8: 55–58.
Zyprian E, Kado CI (1990) Plant Mol. Biol. 15: 245–256.
Author information
Authors and Affiliations
Additional information
Communicated by F. Constabel
Rights and permissions
About this article
Cite this article
Lulsdorf, M.M., Rempel, H., Jackson, J.A. et al. Optimizing the production of transformed pea (Pisum sativum L.) callus using disarmed Agrobacterium tumefaciens strains. Plant Cell Reports 9, 479–483 (1991). https://doi.org/10.1007/BF00232100
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00232100