Abstract
Irones are violet-scented ketonic compounds contained in the rhizome of certain species of iris. As cultivation of the iris tends to decrease, a selection program has been initiated to find the best performing clones in terms of growth and yield. Parallel to this selection, in vitro regeneration studies have been carried out in order to multiply interesting clones. A method of rapid multiplication by somatic embryogenesis associated with multibudding was developed. Callus was obtained from leaf bases, flower pieces or rhizome apices; the best explants were flower pieces. The induction media used to obtain embryogenic callus were Murashige & Skoog (1962) media. Assays with adding of proline in these media have showed that it could double the yield of embryogenic callus. The embryogenic expression medium was the Knudson's orchid agar (Knudson 1946) medium. Conformity of the plants obtained was checked by comparing their chemotypes with those of the mother plants.
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Abbreviations
- AIB:
-
indolyl butyric acid
- BAP:
-
6-benzylaminopurine
- Kin:
-
6-furfurylaminopurine
- KN:
-
Knudson's orchid Agar
- MS:
-
Murashige & Skoog medium
- NAA:
-
naphthaleneacetic acid
- N6:
-
Chu et al. medium
- Pro:
-
proline
- 2,4-D:
-
dichlorophenoxyacetic acid
References
Armstrong CL, Green CE (1985) Planta 164: 207–214
Belcour B, Courtois D, Ehret C, Pétiard V (1993) Phytochemistry 34: 1313–1315
Chu CC, Wang CC, Sun CS, Hsu C, Yin KC, Chu CY, Bi FY (1975) Sci Sin (Peking) 18: 659–668
Fujino M, Fujimura T, Hamada K (1972) J Japan Soc Hort Sci 41 (1): 66–71
Garnero J, Joulain D, Buil P (1978) Rivista Italiana EPPOS 40: 568–590
Gozu Y, Yokoyama M, Nakamura M, Namba R, Yomogida K, Yanagi M, Nakamura S (1993) Plant Cell Reports 13: 12–16
Hussey G (1976) Scient Hort 4: 163–165
Jéhan H, Courtois D, Ehret C, Lerch K, Pétiard V (1994) C R Aca Sci (Paris), in press
Knudson L (1946) Bull Amer Orchid Soc 15: 214–217
Laublin G, Hargurdeep S, Cappadocia S, Cappadocia M (1991) Plant Cell Tiss Org Cult 27: 15–21
Meyer MM, Fuchigama LH, Roberts AN (1975) Hortscience 10: 479–480
Murashige T, Skoog F (1962) Physiol Plant 15: 473–497
Radojevic L, Sokic O, Tucic B (1987) Ada Hort 212: 719–723
Radojevic L, Subotic A (1992) J Plant Physiol 139: 690–696
Reuther G (1977) Ber Deutsch Bot Ges Bd 90: 417–437
Simonet M (1932) Bull Biol France et Belgique 105: 255–444
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Communicated by A. M. Boudet
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Jéhan, H., Courtois, D., Ehret, C. et al. Plant regeneration of Iris pallida Lam. and Iris germanica L. via somatic embryogenesis from leaves, apices and young flowers. Plant Cell Reports 13, 671–675 (1994). https://doi.org/10.1007/BF00231621
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DOI: https://doi.org/10.1007/BF00231621