Abstract
Embryogenic callus cultures of ginger were induced from young leaf segments taken from in vitro shoot cultures. Among the four auxins tested in Murashige & Skoog medium, dicamba at 2.7 μM was most effective in inducing and maintaining embryogenic cultures. Efficient plant regeneration was achieved when embryogenic cultures were transferred to Murashige & Skoog medium containing 8.9 μM benzyladenine. Histological studies revealed various stages of somatic embryogenesis characteristic of the monocot system. The in vitro-raised plants have been established in soil.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- BA:
-
benzyladenine
- 2,4-d :
-
2,4-dichlorophenoxyacetic acid
- IAA:
-
indole-3-acetic acid
- MS:
-
Murashige and Skoog
- NAA:
-
naphthaleneacetic acid
References
Balachandran SM, Bhat SR & Chandel KPS (1990) In vitro clonal multiplication of turmeric (Curcuma spp) and ginger (Zingiber officinale Rosc.). Plant Cell Rep. 8: 521–524
Bhagyalakshmi & Singh NS (1988) Meristem culture and micropropagation of a variety of ginger (Zingiber officinale Rosc.) with a high yield of oleoresin. J. Hort. Sci. 63: 321–327
Bhaskaran S & Smith RH (1990) Regeneration in cereal tissue cultures: A review. Crop. Sci. 30: 1328–1336
Conger BV, Hanning GE, Gray DJ & McDaniel JK (1983) Direct embryogenesis from mesophyll cells of orchardgrass. Science 221: 850–851
Conger BV, Novak FJ, Afza R & Erdelsky K (1987) Somatic embryogenesis from cultured leaf segments of Zea mays. Plant Cell Rep. 6: 345–347
Dekkers AJ, Rao AN & Goh CJ (1991) In vitro storage of multiple shoot cultures of gingers at ambient temperature of 24–29 degrees-C. Scientia Hort. 47: 157–167
Feder N & O'Brien TP (1968) Plant microtechnique: some principles and new methods. Amer. J. Bot. 55: 123–142
Guiderdoni E & Demarly Y (1988) Histology of somatic embryogenesis in cultured leaf segments of sugarcane plantlets. Plant Cell Tiss. Org. Cult. 14: 71–88
Lu C & Vasil IK (1981) Somatic embryogenesis and plant regeneration from leaf tissues of Panicum maximum Jacq. Theor. Appl. Genet. 59: 275–280
Malamug JJF, Inden H & Asahira T (1991) Plantlet regeneration and propagation from ginger callus. Scientia Hort. 48: 89–97
Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497
Nayak P & Sen SK (1989) Plant regeneration through somatic embryogenesis from suspension cultures of a minor millet, Paspalum scrobiculatum. Plant Cell Rep. 8: 296–299
Noguchi Y & Yamakawa O (1988) Rapid clonal propagation of ginger (Zingiber officinale Rosc.) by roller tube culture. Jpn J. Breed. 38: 437–442
Novak FJ, Afza R, VanDuren M, Perea-Dallos M, Conger BV & xiaolang T (1989) Somatic embryogenesis and plant regeneration in suspension cultures of dessert (AA and AAA) and cooking (ABB) bananas (Musa spp). Bio/Technology 7: 154–159
Vasil IK (1988) Progress in the regeneration and genetic manipulation in crops. Bio/Technology 6: 397–402
Vasil V, Vasil IK & Lu C (1984) Somatic embryogenesis in long-term callus cultures of Zea mays L. (Gramineae). Amer. J. Bot. 71: 158–161
Wang H (1989) In vitro clonal propagation of ginger sprouts. Acta Bot. Yunnanica 11: 231–233
Wernicke W & Brettell RIS (1980) Somatic embryogenesis from Sorghum bicolor leaves. Nature 287: 138–139
Wernicke W & Milkovits L (1984) Developmental gradients in wheat leaves-response of leaf segments in different genotypes cultured in vitro. J. Plant Physiol. 115: 49–58
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kackar, A., Bhat, S.R., Chandel, K.P.S. et al. Plant regeneration via somatic embryogenesis in ginger. Plant Cell Tiss Organ Cult 32, 289–292 (1993). https://doi.org/10.1007/BF00042291
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00042291